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FANA vs shRNA

Workflow: FANA vs shRNA

FANA Treatment Workflow

01

Seed cells in the desired format the day before treatment

02

Reconstitute lyophilized FANA ASOs in the appropriate amount of water or buffer

03

Add FANA ASOs to the cell culture medium overlaying the cells

04

Analyze FANA treated cells 24-72 hours after treatment

shRNA (Transduction) Workflow

01

Clone shRNA into the appropriate plasmid

02

Seed cells in the desired format the day before treatment

03

Dilute transfection reagent as recommended by the manufacturer

04

Dilute plasmid DNA as recommended by the manufacturer

05

Combine the diluted plasmid DNA with the diluted transfection reagent

06

Incubate for 5 min

07

Add the plasmid-transfection reagent complexes to the cells

08

Analyze shRNA-transfected cells 24-72 hr after transfection

01

Package shRNA into viral particles

02

Transduce cells with viral particles

03

Select transduced cells with antibiotic (5-7 days)

04

Select clones

05

Analyze shRNA-transfected cells 24-72 hr after transfection