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FANA vs shRNA

Workflow: FANA vs siRNA

FANA Treatment

01

Seed cells in the desired format the day before treatment

02

Reconstitute lyophilized FANA ASOs in the appropriate amount of water or buffer

03

Add FANA ASOs to the cell culture medium overlaying the cells

04

Analyze FANA treated cells 24-72 hours after treatment

siRNA Transfection

01

Seed cells in the desired format the day before treatment

02

Dilute transfection reagent as recommended by the manufacturer

03

Dilute siRNA as recommended by the manufacturer

04

Combine the diluted siRNA with the diluted transfection reagent

05

Incubate

06

Add the siRNA-transfection reagent complexes to the cells

07

Analyze siRNA-transfected cells 24-72 hr after transfection