In vivo delivery protocol
The following protocol can be used for AUMsilence, AUMantagomir, AUMmirblocker, AUMmimic, AUMlnc, AUMskip and AUMblock.
Lipid-based transfection and electroporation are widely utilized, conventional methods to deliver siRNA into the cells. However, in many primary cells, particularly immune cells, hematopoietic cells and neurons, lipid reagents and electroporation are associated with high toxicity and poor transfection efficiency. Alternative delivery methods, such as viral vectors, require laborious optimization and viral production steps, and carry associated risk of genome integration.
The in vivo-ready FANA Antisense Oligonucleotides (FANA ASOs) are highly purified for direct delivery into animal models. FANA ASOs have unique, state-of-the-art chemical modifications that allow for simple and highly efficient delivery into animals without the need of special formulations. FANA’s modifications allow them to be efficiently taken up by the target tissues, and significantly increase their stability and their resistance to endonucleases.