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FANA ASO Calculator

FANA ASO Calculator

FANA ASO Calculator

We have generated this table to assist first time customers with yield amounts of FANA ASOs and final working concentrations.


Cell Media Volume nmoles required for 1 uM final working concentration nmoles required for 5 uM final working concentration nmoles required for 25 uM final working concentration
100 uL (96 Well) 0.1 0.5 2.5
200 uL (48 Well) 0.2 1 5
500 uL (24 Well) 0.5 2.5 12.5
1000 uL (12 Well) 1 5 25
2000 uL (6 Well) 2 10 50

Kindly find corresponding well media volume and find nanomoles of FANA ASO required for recommended final working concentrations of 1 uM, 5 uM, and 25 uM.

As gymnotic delivery does not require the use of transfection reagents, higher concentrations are required to load the cell’s endogenous endosomes for self-delivery. Standard working concentrations range from 1 uM – 10 uM, depending on cell type and gene. In some cases, titrating up to 25 uM may yield favorable results.