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Self-delivery (Gymnotic delivery): Uptake of the AUMsilence ASOs without a delivery agent. No formulation, conjugate or viral vector (like AAV) needed for delivery.
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Ideal for primary cells or difficult to transfect cell lines (no formulation, conjugate or viral vector needed for delivery). Works very well with hard-to-transfect cells especially primary cells which depict true biology (T- cells, B-cells, neuronal cultures and others).
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AUMsilence ASO Treatment Workflow
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Ideal for in vivo studies (no formulation, conjugate or viral vector needed for delivery).
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Can work with fish, insect, and amphibian models (no formulatio or conjugates needed for delivery).
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Non-toxic and do no cause cell death. No need to grow excessive number of cells (typically there is a need to do grow excessive number of cells when we use transfection agents to compensate for high cell death caused by these toxic reagents).
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Resistant to degradation by serum and cellular nucleases significantly improves duration of activity. AUMsilence oligos do not degrade (up to several weeks/months)
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Long term sustained silencing (can be used as an alternative for stable cell lines).
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Low non-specific protein binding decreases toxicity.
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Does not alter the biology of cells and the experiment (as in case of transfection and delivery reagents).
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Ability to bind to RNA target with high affinity and specificity.
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No RISC associated off target effects (as in case of siRNAs).
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Effective in low concentrations; high bioavailability.
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Excellent reproducibility.
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Saves significant time and resources.
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Cost effective and efficient.
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Super easy to use (very convenient protocol).