Seed cells in the desired format the day before treatment
Reconstitute lyophilized AUMsilence ASOs in the appropriate amount of water or buffer
Add AUMsilence ASOs to the cell culture medium overlaying the cells
Analyze AUMsilence oligo treated cells 24-72 hours after treatment
Clone shRNA into the appropriate plasmid
Dilute transfection reagent as recommended by the manufacturer
Dilute plasmid DNA as recommended by the manufacturer
Combine the diluted plasmid DNA with the diluted transfection reagent
Incubate for 5 min
Add the plasmid-transfection reagent complexes to the cells
Analyze shRNA-transfected cells 24-72 hr after transfection
Package shRNA into viral particles
Transduce cells with viral particles
Select transduced cells with antibiotic (5-7 days)
Select clones