Pathway Human Homo sapiens

Processing of Intronless Pre-mRNAs

R-HSA-77595 in Reactome release 97: under Processing of Capped Intronless Pre-mRNA, with 20 genes placed in it by the mapping files and 0 child pathways in the hierarchy.

The same number in the other species

Reactome writes the mouse and rat events it infers from a human pathway under the human number with the species token. A door opens only where that species' own list in this release holds the id: R-MMU-77595 (mouse), R-RNO-77595 (rat). Whether the event was inferred from this one is what the record says.

The record
The genes
Parents and children

Scripts are off

This page reads the pathway's record from its source while you read the rest; with scripts off the card stays at its loading line. Reload with scripts on to see it, or open the source's own page from the link in the card.

01The record

Reactome's own record of this pathway

What this tells you

The pathway list, the hierarchy and the genes on this page are read from the files Reactome publishes for Reactome release 97, built into this site on 2026-09-09: the pathway list, the hierarchy relationship file and the two gene mapping files. The record card is the one live read, Reactome's own record of this pathway.

Reactome's download page describes the mapping files: Mapping files link the source database identifier to the lowest level pathway diagram or subset of the pathway, all levels of the pathway hierarchy or database identifier to all reactions. [R13] The gene list here is the all-levels mapping of NCBI Gene ids, filled from the all-levels mapping of Ensembl ids where the NCBI file has no row for a gene; each row names which file it came from by the shape of its source id. Each row carries the evidence codes the file writes for it, as written and unranked, and both where the file writes both; no page of Reactome's documentation the survey read defines the codes, so this page does not expand them. A gene id the identity files do not name is kept as the source's own id with no door rather than turned into a symbol.

On citing what a search finds, Reactome says: It should be remembered that while we strive to contain the most current and accurate data, Reactome should not be used in citations where other primary sources of information are available. [R12] The record card prints the literature Reactome attaches to a curated pathway for that reason. The data are public domain: All data in the Reactome database and files derived from that data are licensed under the Creative Commons Public Domain Dedication (CC0). User may copy, modify, and distribute these data, even for commercial purposes, without asking for permission. Attribution is encouraged but not required. [R10] The pathway illustrations are licensed apart, under CC BY 4.0, and none is shown here.

A gene on this list is one Reactome places in this pathway in this release, and that is all the row says: it does not say the gene is expressed in a tissue or associated with a disease, which are the other two explorers' questions, read from other sources. Reactome's papers of record are [R01] [R02].

[R01] Ragueneau E, Gong C, Sinquin P, Sevilla C, Beavers D, Grentner A, et al. (2026). The Reactome Knowledgebase 2026. Nucleic Acids Research 54:D673-D681. PMID 41251150, doi 10.1093/nar/gkaf1223.
[R02] Milacic M, Beavers D, Conley P, Gong C, Gillespie M, Griss J, et al. (2024). The Reactome Pathway Knowledgebase 2024. Nucleic Acids Research 52:D672-D678. PMID 37941124, doi 10.1093/nar/gkad1025.
[R10] Reactome, reactome.org. License Agreement. https://reactome.org/license, read 2026-09-09.
[R12] Reactome, reactome.org. Citing us. https://reactome.org/cite, read 2026-09-09.
[R13] Reactome, reactome.org. Download. https://reactome.org/download-data, read 2026-09-09.

Stable id: R-HSA-77595.4
Class: Pathway
Species: Homo sapiens (human)
Inferred: no: a curated human pathway
Disease pathway: no
Released: 2004-07-06
Last updated: 2011-09-30
Events inside: 5, counted from the record

The 3' ends of eukaryotic mRNAs are generated by posttranscriptional processing of an extended primary transcript. For almost all RNAs, 3' processing consists of two steps: The mRNA is first cleaved at a particular phosphodiester bond downstream of the coding sequence. The upstream fragment then receives a poly(A) tail of approximately 250 adenylate residues whereas the downstream fragment is degraded. The two partial reactions are coupled so that reaction intermediates are usually undetectable. While 3' processing can be studied as an isolated event in vitro, it appears to be connected to transcription, splicing and transcription termination in vivo.

Literature Reactome attaches

Integrating mRNA processing with transcription.Cell, 2002, vol. 108, p. 501-12PubMed 11909521

Reactome's record lists 13 orthologous events, R-RNO-77595 (Rattus norvegicus), R-MMU-77595 (Mus musculus) among the species this site serves, and 11 in species this site opens no page for.

Reactome ContentService, this pathway's record · Reactome v97 · read 2026-10-01 ET · Reactome pathway browser"Processing of Intronless Pre-mRNAs". Reactome, release 97, https://reactome.org/PathwayBrowser/#/R-HSA-77595 with StableID: R-HSA-77595.4 (accessed 2026-10-01 ET). Reactome version 97 (CC0).

Reading this pathway's record from Reactome (the pathway record).Still reading. A first read of a pathway can take a while; this page waits up to 35 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.

02The genes

Genes Reactome places in this human pathway

The mapping files place 20 genes in this human pathway; showing 1 to 20, in pages of 100, sorted by symbol for reading. The order carries no ranking.

Genes Reactome places in this human pathway, page 1 of 1
Gene	Authority id	Mapping file id	Evidence codes
GeneCLP1	AuthorityHGNC:16999	Mapping file id10978 NCBI file	EvidenceTAS
GeneCPSF1	AuthorityHGNC:2324	Mapping file id29894 NCBI file	EvidenceTAS
GeneCPSF2	AuthorityHGNC:2325	Mapping file id53981 NCBI file	EvidenceTAS
GeneCPSF3	AuthorityHGNC:2326	Mapping file id51692 NCBI file	EvidenceTAS
GeneCPSF4	AuthorityHGNC:2327	Mapping file id10898 NCBI file	EvidenceTAS
GeneCPSF6	AuthorityHGNC:13871	Mapping file id11052 NCBI file	EvidenceTAS
GeneCPSF7	AuthorityHGNC:30098	Mapping file id79869 NCBI file	EvidenceTAS
GeneCSTF1	AuthorityHGNC:2483	Mapping file id1477 NCBI file	EvidenceTAS
GeneCSTF2	AuthorityHGNC:2484	Mapping file id1478 NCBI file	EvidenceTAS
GeneCSTF2T	AuthorityHGNC:17086	Mapping file id23283 NCBI file	EvidenceTAS
GeneCSTF3	AuthorityHGNC:2485	Mapping file id1479 NCBI file	EvidenceTAS
GeneFIP1L1	AuthorityHGNC:19124	Mapping file id81608 NCBI file	EvidenceTAS
GeneNCBP1	AuthorityHGNC:7658	Mapping file id4686 NCBI file	EvidenceTAS
GeneNCBP2	AuthorityHGNC:7659	Mapping file id22916 NCBI file	EvidenceTAS
GeneNUDT21	AuthorityHGNC:13870	Mapping file id11051 NCBI file	EvidenceTAS
GenePABPN1	AuthorityHGNC:8565	Mapping file id8106 NCBI file	EvidenceTAS
GenePAPOLA	AuthorityHGNC:14981	Mapping file id10914 NCBI file	EvidenceTAS
GenePCF11	AuthorityHGNC:30097	Mapping file id51585 NCBI file	EvidenceTAS
GeneSYMPK	AuthorityHGNC:22935	Mapping file id8189 NCBI file	EvidenceTAS
GeneWDR33	AuthorityHGNC:25651	Mapping file id55339 NCBI file	EvidenceTAS

Evidence codes on this page: TAS, as the mapping file writes them; a row carrying two was written twice by the file, once under each. The mapping file id says which file placed the gene: an NCBI Gene id from NCBI2Reactome_All_Levels.txt, an Ensembl gene id from Ensembl2Reactome_All_Levels.txt. The two files can disagree about a code, so a row's codes are the one file's view. Measured by this site's build over this release on 2026-09-09: of the 138,908 human pathway-gene pairs both files place, 207 (0.149 per cent, over 47 genes) carry TAS in the Ensembl file where the NCBI rows carry IEA alone.

Reactome mapping files, the human rows for this pathway · Reactome release 97 · read 2026-09-09 · Reactome downloads"NCBI2Reactome_All_Levels.txt" and "Ensembl2Reactome_All_Levels.txt", Reactome, release 97, https://reactome.org/download-data/ (date of access 2026-09-09). Data CC0; attribution encouraged.

03The hierarchy

Parents and children in this release's hierarchy

Parents

Processing of Capped Intronless Pre-mRNAR-HSA-7506729 genes

Children

None: no pathway of this release's list names this one as a parent.

Reactome's hierarchy is a graph rather than a tree: a pathway can sit under more than one parent, and the gene counts are each pathway's own placements at every level under it, so a parent's count is not the sum of its children's.

Reactome, the human pathway list and hierarchy relationship file · Reactome release 97 · read 2026-09-09 · Reactome downloads"ReactomePathways.txt" and "ReactomePathwaysRelation.txt", Reactome, release 97, https://reactome.org/download-data/ (date of access 2026-09-09). Data CC0; attribution encouraged.