Made to order, by target or sequence
Conventional oligonucleotides, to your target or your sequence
2'-MOE, locked nucleic acid and 2'-OMe, in three purification tiers
What these are
Conventional antisense chemistry
2'-MOE, locked nucleic acid and 2'-OMe are conventional antisense chemistries, each named for a modification of the ribose sugar. 2'-MOE and locked nucleic acid are ordered as gapmers for RNase H knockdown, and all three are ordered without a gap, as steric blockers. They carry no brand name, because they are conventional chemistry rather than a branded AUM BioTech product. In cell culture they are delivered with a transfection reagent, by electroporation, or by unassisted uptake at a higher concentration; in animals they are dosed in saline.
Ordering starts under Conventional oligos on the order page. Name a target and the sequence is designed using the AUMsilence platform, priced there, or send your own sequence, priced by quotation.
Where delivery into the cell is the difficulty rather than the chemistry, the self-delivering products enter without a transfection reagent: AUMsilence
Three chemistries
Mechanism and selection
2'-MOE
A methoxyethyl group on the 2' position of the ribose raises affinity for the target RNA and resists nucleases. For RNase H knockdown it is built as a gapmer: 2'-MOE wings around a central DNA gap.
Selected for
A knockdown gapmer of conventional length, and a published 2'-MOE gapmer remade as described.
Locked nucleic acid
A methylene bridge holds the ribose in the conformation RNA itself takes, which raises affinity per residue more than 2'-MOE or 2'-OMe does. Knockdown designs are gapmers with short locked wings; steric block and microRNA inhibition use a mixmer with no gap.
Selected for
A short accessible window, a structured target region, and a published design already built on locked nucleic acid.
2'-OMe
A methyl group on the 2' position of the ribose raises affinity and resists nucleases. A 2'-OMe oligonucleotide modified at every position does not support RNase H cleavage, so it occupies its site rather than cleaving the transcript: steric block, splice switching and microRNA inhibition.
Selected for
Occupying a site on the RNA rather than cleaving the transcript, and remaking a validated 2'-OMe sequence.
Purification
Three purification tiers
Every chemistry is sold in the same three tiers. The tier decides how much of what arrives is full-length product, and it sets the study model. Desalt and RPC are sold for cellular work, HPLC for primary cells and animal models.
desalt
Removes the salts and small-molecule by-products left after synthesis, cleavage and deprotection. The full-length product remains together with the shorter failure sequences that incomplete coupling leaves behind.
- Study model
- in vitro, cellular studies
RPC
Reversed-phase purification: the full-length oligo is separated from the shorter failure sequences by hydrophobicity. A step between desalt and HPLC, for cellular work that needs more of the tube to be full length.
- Study model
- in vitro, cellular studies
HPLC
High-performance liquid chromatography resolves the full-length product from the failure sequences on a column, the highest purity of the three tiers. HPLC is the grade bought for primary cells and animal models.
- Study model
- in vitro and in vivo
Yields
The yield ladder
The same nine yields are sold in each of the three tiers, for every chemistry.
- 5
- 10
- 25
- 50
- 100
- 200
- 250
- 500
- 1000
Yields above 1000 nmol are quoted through bulk synthesis. No fluorescently labeled version of these chemistries is sold online.
Ordering
How an order is placed
Step 1: On the order page, under Conventional oligos, choose Design it for me or I have the sequence.
Step 2: Under Design it for me, name the target and the purpose. The sequence is designed using the AUMsilence platform. 2'-MOE and locked nucleic acid are the knockdown chemistries; all three are used for steric block, splice switching and microRNA inhibition.
Step 3: Under I have the sequence, the sequence is pasted as it is to be made. It is yours: the platform does not design it and does not alter it.
Step 4: The chemistry, the purification tier and the delivered yield are chosen next. The tier sets the study model.
Step 5: A designed oligonucleotide is priced on the order page at the yield and tier chosen. A sequence of your own is priced by quotation, as is any 2'-MOE or locked nucleic acid position in a pasted sequence.
Step 6: Payment is by card or by purchase order, and a quotation can be taken to a purchasing office.
Above 1000 nmol, or another modification
Bulk synthesis covers larger yields, further sugar and backbone modifications, and purification and quality documentation beyond the three tiers here, by quotation.
For research use only. Not for use in diagnostic or therapeutic procedures.