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High-throughput screening

A screen from a list of targets

Name the targets and the species: genes, long non-coding RNAs or microRNAs. AUM designs the sequences against each, makes them HPLC-purified in the chemistry you choose, and delivers them in tubes or plated with the controls included, priced per oligo, from 5 targets to 384.

Start with your listBulk synthesis

Choose the target type, name the targets, choose how the panel is made, read the plate back, and send the request.

01 Target type

One list, one target type. Each door names the product designed against it.

02 Species and gene list

Species

An example, not your list

24 human genes, 3 sequences each, to show how a screen is laid out and priced.

From 5 to 384 genes, each checked against the mRNA index the search box uses: the official symbol, a previous symbol or an alias, in the species chosen. Typed, pasted, or from a file: one gene symbol per row, or the first column of a CSV. A file of up to 500 rows and 1 MB, as .txt, .csv or .tsv; a spreadsheet workbook is refused, so save it as CSV first.

or drop one on this box

24 genes: 0 resolved, 24 resolving.

The list, with what the symbol index made of each row
RowTypedTargetStatus
1TP53resolvingchecking the index
2EGFRresolvingchecking the index
3KRASresolvingchecking the index
4BRAFresolvingchecking the index
5MYCresolvingchecking the index
6PTENresolvingchecking the index
7AKT1resolvingchecking the index
8PIK3CAresolvingchecking the index
9CDK4resolvingchecking the index
10CDK6resolvingchecking the index
11CCND1resolvingchecking the index
12RB1resolvingchecking the index
13BRCA1resolvingchecking the index
14BRCA2resolvingchecking the index
15ATMresolvingchecking the index
16MTORresolvingchecking the index
17CHEK1resolvingchecking the index
18CHEK2resolvingchecking the index
19MDM2resolvingchecking the index
20BCL2resolvingchecking the index
21BAXresolvingchecking the index
22CASP3resolvingchecking the index
23STAT3resolvingchecking the index
24JUNresolvingchecking the index

A screen starts at 5 genes. A shorter list, or one gene only, is a single-target order. The order page prices a single set of sequences.

03 Chemistry

AUM designs the sequences with its platform in every chemistry offered for the mRNA screen.

04 Purification

HPLC, the purification every screening product is made in, in tubes and on plates alike: one grade for the whole screen.

05 Sequences per gene

3 as standard: a phenotype seen with more than one sequence is told from an off-target effect of one.

06 Format

A delivery format at no difference in price: every oligo is priced the same in tubes and on a plate. A plate is available from 48 oligos (genes times sequences per gene); below that the order is delivered in tubes. A plate is filled down the columns with its control wells at the end, a gene never split across two.

07 Controls, included

On every plate the class's full set, in the last 3 wells of the fill order, at no charge, from the catalogue's own HPLC controls.

  • Scramble controlAUM-SCR-B-10
  • Positive controlAUM-POS-B-10
  • Labeled scramble controlAUM-SCR-B-10-LAB-FAM

08 The plate, drawn

One 96-well plate · 31 genes each at 3 per gene, 3 control wells of a block of 3

1
2
3
4
5
6
7
8
9
10
11
12
A
B
C
D
E
F
G
H

A1: TP53, human, sequence 1 of 3

  • gene, sequence number in the well
  • control (S scramble, P positive, L labeled)
  • spare

Filled down the columns from A1. 24 genes on this plate, room for 7 more, 21 spare wells. Arrow keys move between wells; Page Up and Page Down change the plate.

09 The manifest

Manifest, 75 rows: one per well or tube.

The manifest: plate, well, target type, target, species, sequence number and product for every well or tube
PlateWellTypeTargetSpeciesSeqProduct
1A1mRNATP53human1on quotation
1B1mRNATP53human2on quotation
1C1mRNATP53human3on quotation
1D1mRNAEGFRhuman1on quotation
1E1mRNAEGFRhuman2on quotation
1F1mRNAEGFRhuman3on quotation
1G1mRNAKRAShuman1on quotation
1H1mRNAKRAShuman2on quotation
1A2mRNAKRAShuman3on quotation
1B2mRNABRAFhuman1on quotation
1C2mRNABRAFhuman2on quotation
1D2mRNABRAFhuman3on quotation
1E2mRNAMYChuman1on quotation
1F2mRNAMYChuman2on quotation
1G2mRNAMYChuman3on quotation
1H2mRNAPTENhuman1on quotation
1A3mRNAPTENhuman2on quotation
1B3mRNAPTENhuman3on quotation
1C3mRNAAKT1human1on quotation
1D3mRNAAKT1human2on quotation
1E3mRNAAKT1human3on quotation
1F3mRNAPIK3CAhuman1on quotation
1G3mRNAPIK3CAhuman2on quotation
1H3mRNAPIK3CAhuman3on quotation
1A4mRNACDK4human1on quotation
1B4mRNACDK4human2on quotation
1C4mRNACDK4human3on quotation
1D4mRNACDK6human1on quotation
1E4mRNACDK6human2on quotation
1F4mRNACDK6human3on quotation
1G4mRNACCND1human1on quotation
1H4mRNACCND1human2on quotation
1A5mRNACCND1human3on quotation
1B5mRNARB1human1on quotation
1C5mRNARB1human2on quotation
1D5mRNARB1human3on quotation
1E5mRNABRCA1human1on quotation
1F5mRNABRCA1human2on quotation
1G5mRNABRCA1human3on quotation
1H5mRNABRCA2human1on quotation
1A6mRNABRCA2human2on quotation
1B6mRNABRCA2human3on quotation
1C6mRNAATMhuman1on quotation
1D6mRNAATMhuman2on quotation
1E6mRNAATMhuman3on quotation
1F6mRNAMTORhuman1on quotation
1G6mRNAMTORhuman2on quotation
1H6mRNAMTORhuman3on quotation

Optional. 2,000 characters at most.

For research use only. Not for use in diagnostic or therapeutic procedures.

What a screen is

Many targets, one order

A screen is one order against many targets at once, and the target type is chosen first so that one product is never confused with another. A gene list becomes an mRNA screen with AUMsilence sdASO: each gene gets 3 designed sequences as standard, 1 to 5 by choice. A list of long non-coding RNAs becomes a panel with AUMlnc sdASO, 3 sequences per transcript as standard, nuclear or cytoplasmic. A list of mature microRNAs becomes a panel with AUMantagomir sdASO: one mature sequence, one antagomir, named with its arm.

Every self-delivering product needs no transfection reagent, in culture or in vivo; the transfection-optimized AUMsilence toASO, AUMlnc toASO and AUMantagomir toASO, and the 2'-MOE and locked nucleic acid gapmers, are delivered by you. The gapmers are offered for an mRNA or a long non-coding RNA: a gapmer recruits RNase H1 against a long transcript, and a mature microRNA is inhibited by a steric-block antagomir instead. Every screening product is HPLC-purified and priced per oligo, so a plate is a delivery format at no difference in price, available from 48 oligos; below that an order is delivered in tubes. Every plate carries its class's full set of controls, included; a tube order includes the class's scramble control. Every product is designed by the same platform against the same list, and the request names every sequence: the target, its type, the species, the sequence number and the well it sits in.

Ordering

How it works

  1. Step 1: Choose the target type: mRNA, long non-coding RNA or microRNA. Each door names the product designed against it, and the chemistries, the sequences per target and the controls follow from it.

  2. Step 2: Paste or upload the list and choose the species. Every row is checked against the index the search box uses for that target type, and a row the index does not know is shown with the nearest names to pick from, never dropped; a microRNA named without its arm is offered the arms miRBase lists.

  3. Step 3: Choose the chemistry, the sequences per target and the format: tubes, or, from 48 oligos, a 96-well or a 384-well plate, HPLC-purified either way and priced per oligo, with the full control set included on every plate and the scramble control in tubes.

  4. Step 4: Read the plate back, well by well, and download the manifest: one row per well or tube with the target type, the target, the species, the sequence number and the product. Then send the request. Where the price list holds a screening record the price is computed from it and shown line by line; where it does not, the request goes to the team on quotation as it stands.

Applications

High-throughput screening applications

  • Target validation

    Validate potential drug targets by screening gene families or pathway components simultaneously.

  • Pathway analysis

    Dissect complex signaling pathways by knocking down multiple components in parallel.

  • Phenotypic screening

    Identify genes involved in specific cellular phenotypes through systematic knockdown.

  • Disease modeling

    Create disease models by targeting disease-associated gene sets identified from GWAS or transcriptomics.

  • Non-coding RNA panels

    Inhibit a set of microRNAs, one antagomir each, or knock down a set of long non-coding RNAs, in one plate with its own controls.

Above 384 targets, or a genome-wide set

The page takes 5 to 384 targets in one request. For a larger set, or a screen designed with us, book a consultation or write to customer care.

Schedule a scientific consultationContact

For research use only. Not for use in diagnostic or therapeutic procedures.