Your sequences, in bulk
Custom bulk ASO synthesis
Paste or upload a list of 5 or more sequences in any notation. The oligonucleotides are made HPLC-purified in the chemistry you choose, in tubes or on plates at the same price per oligo.
Paste your sequences in any notation, choose how they are made, read the list back, and send the request.
01 Your sequences
An example, not your list
a few sequences in the notations the reader accepts, to show how a list is read back.
From 5 to 384 sequences, each read by the reader the sequence builder uses: plain letters, slash codes, bracket codes, mA or +A prefixes, an asterisk or an s for a phosphorothioate linkage, 8 to 110 nucleotides. Typed, pasted, or from a file: one sequence per row, a name before a comma or a tab if you like. A file of up to 500 rows and 1 MB, as .txt, .csv or .tsv; a spreadsheet workbook is refused, so save it as CSV first.
5 sequences: 2 read whole at the list's price, 3 read and quoted with a reason of their own.
| Row | Name | Read back | Length | Chemistry | Status |
|---|---|---|---|---|---|
| 1 | example-1 | [eG][eC][eA][eT][eT]CAGTCAGTCA[eG][eT][eC][eA][eG] | 20 | 2'-MOE | read whole; takes the list's price |
| 2 | example-2 | [5PHOS][eC][eA][eG][eT][eC]AGTCAGTCAG[eT][eC][eA][eG][eT] | 20 | 2'-MOE | On quotation: An end modification is quoted. |
| 3 | example-3 | [mG]*[mC]*[mA]*[mU]*UCAGTCAGTCA[mG]*[mU]*[mC]*[mA]*[mG] | 20 | 2'-OMe | On quotation: Names 2'-OMe, not the chemistry chosen. |
| 4 | example-4 | [lG][lC][lA]TTCAGTCAGTCAG[lT][lC] | 18 | locked nucleic acid | On quotation: Names locked nucleic acid, not the chemistry chosen. |
| 5 | example-5 | [eT][eG][eA][eC][eT]GACTGACTGA[eC][eT][eG][eA][eC] | 20 | 2'-MOE | read whole; takes the list's price |
A bulk list starts at 5 sequences. A shorter list, or one sequence only, is a single-sequence order. The order page prices a single set of sequences.
03 Purification
HPLC, the purification every screening product is made in, in tubes and on plates alike: one grade for the whole screen.
5 oligos on this list: delivered in tubes, a plate being available from 48.
05 Controls, included
In tubes, the class's scramble control alone, one per order, after the samples, at no charge, from the catalogue's own HPLC controls. The positive control and the labeled scramble travel with a plate; the order page sells them on their own.
- Scramble controlMOE_SCR-B-2
06 The manifest
Manifest, 6 rows: one per well or tube.
| Plate | Well | Type | Target | Species | Seq | Product |
|---|---|---|---|---|---|---|
| T1 | sequence | example-1 | human | 1 | on quotation | |
| T2 | sequence | example-2 | human | 1 | on quotation | |
| T3 | sequence | example-3 | human | 1 | on quotation | |
| T4 | sequence | example-4 | human | 1 | on quotation | |
| T5 | sequence | example-5 | human | 1 | on quotation | |
| T6 | control | MOE_SCR-B-2 | MOE_SCR-B-2 |
Optional. 2,000 characters at most.
For research use only. Not for use in diagnostic or therapeutic procedures.
Capabilities
ASO manufacturing capabilities
From research validation to preclinical development
AUM BioTech provides research-grade antisense oligonucleotides with chemical modification capabilities for research and preclinical development. Our scalable manufacturing platform delivers consistent quality from research scale (μmol) through multi-gram production, with validated analytical methods and complete analytical documentation.
Whether you are advancing a target from research validation to preclinical studies, or need larger quantities of a modified ASO for extended in vivo work, our custom synthesis service delivers the exact modifications, scale, and purity you need.
Modifications
Modification capabilities
A bulk order can combine sugar and backbone modifications position by position, from those below.
Sugar modifications
- 2'-O-methyl (2'-OMe)
- Resists nucleases, and an oligonucleotide modified at every position does not support RNase H cleavage
- 2'-O-methoxyethyl (2'-MOE)
- More nuclease resistance than 2'-OMe
- 2'-fluoro (2'-F)
- Raises affinity for the target RNA
- Locked nucleic acid
- More affinity per residue than the other three
Backbone modifications
- Phosphorothioate (PS)
- Resists nucleases and binds proteins, lowers duplex affinity slightly, and can raise toxicity at high concentration
- Phosphodiester (PO)
- The unmodified backbone, digested quickly in serum
- Mixed PS/PO
- Phosphorothioate at the chosen positions only
- Custom chimeric designs
- Sugar and backbone set position by position
Scale
Flexible scale options
Scaling from research to preclinical production
| Scale tier | Quantity range | Typical applications | Turnaround |
|---|---|---|---|
| Research scale | 1-10 μmol | Target validation, initial screening, mechanism studies | 2-3 weeks |
| Pre-clinical | 10-100 μmol | In vivo efficacy, PK/PD studies, dose-response optimization | 3-4 weeks |
| Scale-up | 100-1000 μmol | Preclinical toxicology, formulation development, scale-up material | 6-8 weeks |
| Bulk production | 1-500+ grams | Extended in vivo programs, multi-study preclinical campaigns, reference and stability material | 8-24 weeks |
A bulk order follows the turnaround in this table, not the standard lead time of 10-14 business days the catalogue's products carry.
For a scale beyond the catalogue's ladder, write it in the notes of the request above.
Purification and quality
Research-grade purification & QC
Multiple purification tiers to meet your quality requirements
Purification methods
- Cartridge purification
- Standard desalting
- RP-HPLC
- High purity (>95%)
- IE-HPLC
- Ultra-high purity (>98%, >99%)
Quality assurance
- MALDI-TOF MS
- Molecular weight confirmation
- HPLC analysis
- Purity verification
- Endotoxin testing
- For in vivo applications
- Certificate of analysis
- Complete analytical package
Delivery and storage
Delivery & storage
Ensuring stability from manufacturing to your lab
Shipping format
- Lyophilized powder
- Stable for long-term storage
- Solution format
- Ready-to-use for immediate applications
- Cold chain shipping
- Temperature-controlled delivery
- Custom aliquoting
- Minimize freeze-thaw cycles
Storage recommendations
- Lyophilized
- Store at -20°C, stable for 24+ months
- Solution (sterile)
- Store at -80°C, aliquot to avoid freeze-thaw
- Reconstitution
- Nuclease-free water or PBS recommended
- Stability data
- Available upon request for larger batches
Challenges
Overcoming common ASO development challenges
Solutions for difficult synthesis and scale-up problems
Challenging sequences
Successfully synthesize difficult sequences including G-rich regions, poly-purine stretches, and highly repetitive motifs
Scale-up
Prevent scale-dependent failures through early process optimization and analytical methods that transfer smoothly from development to production
Complex modification patterns
Custom gapmer designs, mixed backbone chemistries and position-specific modifications, set position by position on the order
Analytical method development
Custom analytical method development and validation for complex modification patterns, ensuring thorough characterization from research through preclinical studies
Why AUM BioTech
Why choose AUM BioTech for custom synthesis
Modification expertise
Complete synthesis capabilities for modified ASO chemistries including 2'-O-methoxyethyl (MOE), 2'-O-methyl (OMe), 2'-fluoro modifications with phosphorothioate, phosphodiester, or custom mixed backbone configurations
Scalable manufacturing
Scaling from development (1 μmol) to multi-gram production (500+ grams) with validated processes ensuring batch-to-batch reproducibility and consistent purity profiles
Rapid turnaround
In-house synthesis, with the turnaround for each tier in the scale table above
Partnership approach
A scientist works on the order from the sequence and its chemistry through the analytical documentation and the scale
Quality assurance
Analytical testing including MALDI-TOF MS, HPLC purity analysis, endotoxin testing, and a complete certificate of analysis
Scientific support
Direct access to experienced oligonucleotide chemists for sequence design consultation, modification strategy, analytical method development, and troubleshooting throughout your project lifecycle
The next study, planned with a scientist
A consultation covers the design and its chemistry, the scale, the purification and the analytical documentation, with the chemists who make the material.
For research use only. Not for use in diagnostic or therapeutic procedures.