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Protocols, FAQs, and troubleshooting guides for our RNA silencing technology

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Interactive tools

Online calculators and planning tools for RNA silencing experiments

How much to order

Calculate the amount of ASO an experiment needs, across conditions, time points and technical replicates. The recommended working range, the dilution from stock, and the usage guidelines for both self-delivering (sdASO) and transfection-optimized (toASO) products are on the same page.

CAR-T enhancement guide

Guide to enhancing CAR-T cells with transfection-free gene silencing

Step-by-step experimental protocols

Detailed guides for using self-delivering and transfection-optimized antisense oligonucleotides in your research

AUMsilence sdASO - mRNA knockdown protocol

10 min read

Self-delivering · mRNA · Cell culture

Complete protocol for mRNA knockdown using AUMsilence sdASO in cell cultures without transfection reagents.

AUMantagomir sdASO - miRNA inhibition protocol

12 min read

Self-delivering · miRNA · Inhibition

Detailed protocol for microRNA inhibition using AUMantagomir sdASO - designed for efficient blockade of miRNA function.

AUMlnc sdASO - lncRNA knockdown protocol

12 min read

Self-delivering · lncRNA · Nuclear RNA

Protocol for long non-coding RNA knockdown using AUMlnc sdASO - optimized for targeting nuclear-retained and chromatin-associated lncRNAs.

AUMblock sdASO - steric blocking protocol

12 min read

Self-delivering · Steric blocking · RNA modulation

Detailed protocol for RNA modulation using AUMblock sdASO - designed to bind target RNAs and block protein interactions without recruiting RNase H.

AUMsplice sdASO - exon skipping protocol

12 min read

Self-delivering · Splice modulation · Exon skipping

Protocol for exon skipping and splice modulation using AUMsplice sdASO - redirect splicing to create specific mRNA isoforms or correct disease-causing splice defects in research models.

AUMsilence V+ sdASO - viral RNA knockdown

14 min read

Self-delivering · Viral RNA · Antiviral

Protocol for viral RNA targeting using AUMsilence V+ sdASO - designed for highly efficient knockdown of viral genomes and transcripts in virology research.

In vivo knockdown protocol

15 min read

Animal studies · sdASO · In vivo

Guide for animal studies using self-delivering ASOs, covering formulation, dosing, administration routes, and tissue analysis techniques for effective in vivo gene silencing.

Controls and validation strategies

10 min read

Experimental design · Validation

Essential guide for designing proper controls, validation methods, and data analysis approaches.

Transfection protocol for AUMsilence toASO

12 min read

Transfection-optimized · AUMsilence toASO
Optimized protocol for AUMsilence toASO, which requires transfection reagents, includes reagent selection, complex formation, and delivery optimization for cost-effective gene silencing.

Transfection protocol for AUMantagomir toASO

Transfection-optimized · AUMantagomir toASO
AUMantagomir toASOs are delivered with a transfection reagent, by a lipid, electroporation, calcium phosphate or polymer method; inhibition is confirmed by the de-repression of known miRNA target genes.

Transfection protocol for AUMlnc toASO

Transfection-optimized · AUMlnc toASO
AUMlnc toASOs are delivered with a transfection reagent, by a lipid, electroporation, calcium phosphate or polymer method; knockdown is read by qRT-PCR or RNA-FISH, with nuclear fractionation for nuclear-retained lncRNAs.

For research use only. Not for use in diagnostic or therapeutic procedures.