Configure your RNA-silencing products
Choose a product family, name the target or paste your sequence, then set purification and yield.
Self-delivering antisense, taken up by cells without a transfection reagent: AUMsilence, AUMantagomir, AUMlnc and AUMmimic sdASOs, designed to the targets you name or made from your own sequences.
Quotation request.
01 How do you want to start?
Name the targets and AUM designs, screens and makes the sequences.
10 nmol: enough for 2 wells of a 24-well plate at 10 μM, one treatment each.
How much to order?
A titration at 5 μM, 10 μM and 20 μM in triplicate is 9 wells. The wells are spread over the series.
9 wells: 3 at each of 5 μM, 10 μM and 20 μM.
1 dose: 3 days is within one re-dosing interval.
You have 10 nmol selected. In 500 μL that is 2 treatments at 10 μM.
For 9 wells over 5 μM, 10 μM and 20 μM in one dose the experiment needs 52.5 nmol, or 60.38 nmol with a 15% margin. The 100 nmol yield covers it with 7 treatments left.
Also consider: 50 nmol, the yield below, covers 8 wells like these, not the whole experiment.
You have 10 nmol selected. In 500 μL that is 2 treatments at 10 μM. For 9 wells over 5 μM, 10 μM and 20 μM in one dose the experiment needs 52.5 nmol, or 60.38 nmol with a 15% margin. The 100 nmol yield covers it with 7 treatments left.
Three or five sequences?
- One sequence is the right order when the sequence is already settled: a published design you are reproducing, or one you have used before.
- Three sequences are the standard first pass on a target nobody has silenced: enough to find a working sequence without spending the budget on one.
- Five sequences are for an experiment you cannot easily repeat: primary cells from one donor, an animal cohort, a transcript whose open windows are few.
The chance that at least one sequence works, at three published hit rates:
- 1 of 4 (25%) in Baby et al., Aging Cell, 2020: 58% with three, 76% with five
- 3 of 8 (38%) in Page et al., Journal of Neuroimmunology, 2023: 76% with three, 91% with five
- 11 of 19 (58%) in Akimova et al., Frontiers in Immunology, 2024: 93% with three, 99% with five
Each rate is one published screen's count at its own bar. The arithmetic treats the sequences as independent, which they are not, so every figure is a ceiling.
If none of the five reaches 70% knockdown of the target transcript under the protocol, AUM designs and supplies five more against the same target at no charge, once. The terms say how a claim is made. Terms and conditions
Order the scramble control alongside every time, and a labeled scramble once per cell type.
For research use only. Not for use in diagnostic or therapeutic procedures.