Pathway Human Homo sapiens

Protein methylation

R-HSA-8876725 in Reactome release 97: under Post-translational protein modification, with 19 genes placed in it by the mapping files and 0 child pathways in the hierarchy.

The same number in the other species

Reactome writes the mouse and rat events it infers from a human pathway under the human number with the species token. A door opens only where that species' own list in this release holds the id: R-MMU-8876725 (mouse), R-RNO-8876725 (rat). Whether the event was inferred from this one is what the record says.

The record
The genes
Parents and children

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This page reads the pathway's record from its source while you read the rest; with scripts off the card stays at its loading line. Reload with scripts on to see it, or open the source's own page from the link in the card.

01The record

Reactome's own record of this pathway

What this tells you

The pathway list, the hierarchy and the genes on this page are read from the files Reactome publishes for Reactome release 97, built into this site on 2026-09-09: the pathway list, the hierarchy relationship file and the two gene mapping files. The record card is the one live read, Reactome's own record of this pathway.

Reactome's download page describes the mapping files: Mapping files link the source database identifier to the lowest level pathway diagram or subset of the pathway, all levels of the pathway hierarchy or database identifier to all reactions. [R13] The gene list here is the all-levels mapping of NCBI Gene ids, filled from the all-levels mapping of Ensembl ids where the NCBI file has no row for a gene; each row names which file it came from by the shape of its source id. Each row carries the evidence codes the file writes for it, as written and unranked, and both where the file writes both; no page of Reactome's documentation the survey read defines the codes, so this page does not expand them. A gene id the identity files do not name is kept as the source's own id with no door rather than turned into a symbol.

On citing what a search finds, Reactome says: It should be remembered that while we strive to contain the most current and accurate data, Reactome should not be used in citations where other primary sources of information are available. [R12] The record card prints the literature Reactome attaches to a curated pathway for that reason. The data are public domain: All data in the Reactome database and files derived from that data are licensed under the Creative Commons Public Domain Dedication (CC0). User may copy, modify, and distribute these data, even for commercial purposes, without asking for permission. Attribution is encouraged but not required. [R10] The pathway illustrations are licensed apart, under CC BY 4.0, and none is shown here.

A gene on this list is one Reactome places in this pathway in this release, and that is all the row says: it does not say the gene is expressed in a tissue or associated with a disease, which are the other two explorers' questions, read from other sources. Reactome's papers of record are [R01] [R02].

[R01] Ragueneau E, Gong C, Sinquin P, Sevilla C, Beavers D, Grentner A, et al. (2026). The Reactome Knowledgebase 2026. Nucleic Acids Research 54:D673-D681. PMID 41251150, doi 10.1093/nar/gkaf1223.
[R02] Milacic M, Beavers D, Conley P, Gong C, Gillespie M, Griss J, et al. (2024). The Reactome Pathway Knowledgebase 2024. Nucleic Acids Research 52:D672-D678. PMID 37941124, doi 10.1093/nar/gkad1025.
[R10] Reactome, reactome.org. License Agreement. https://reactome.org/license, read 2026-09-09.
[R12] Reactome, reactome.org. Citing us. https://reactome.org/cite, read 2026-09-09.
[R13] Reactome, reactome.org. Download. https://reactome.org/download-data, read 2026-09-09.

Stable id: R-HSA-8876725.6
Class: Pathway
Species: Homo sapiens (human)
Inferred: no: a curated human pathway
Disease pathway: no
Released: 2016-12-12
Last updated: 2023-03-29
Events inside: 9, counted from the record

Methylation of lysine (Lys) and arginine (Arg) residues on non-histone proteins is a prevalent post-translational modification and important regulator of cellular signal transduction pathways including MAPK, WNT, BMP, Hippo and JAK–STAT. Crosstalk between methylation and other types of post-translational modifications and between histone and non-histone protein methylation is frequent, affecting cellular functions such as chromatin remodelling, gene transcription, protein synthesis, signal transduction and DNA repair (Biggar & Li 2015).

Literature Reactome attaches

Non-histone protein methylation as a regulator of cellular signalling and functionNat. Rev. Mol. Cell Biol., 2015, vol. 16, p. 5-17PubMed 25491103

Reactome's record lists 14 orthologous events, R-RNO-8876725 (Rattus norvegicus), R-MMU-8876725 (Mus musculus) among the species this site serves, and 12 in species this site opens no page for.

Reactome ContentService, this pathway's record · Reactome v97 · read 2026-10-01 ET · Reactome pathway browser"Protein methylation". Reactome, release 97, https://reactome.org/PathwayBrowser/#/R-HSA-8876725 with StableID: R-HSA-8876725.6 (accessed 2026-10-01 ET). Reactome version 97 (CC0).

Reading this pathway's record from Reactome (the pathway record).Still reading. A first read of a pathway can take a while; this page waits up to 35 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.

02The genes

Genes Reactome places in this human pathway

The mapping files place 19 genes in this human pathway; showing 1 to 19, in pages of 100, sorted by symbol for reading. The order carries no ranking.

Genes Reactome places in this human pathway, page 1 of 1
Gene	Authority id	Mapping file id	Evidence codes
GeneCALM1	AuthorityHGNC:1442	Mapping file id801 NCBI file	EvidenceTAS
GeneCALM2	AuthorityHGNC:1445	Mapping file id805 NCBI file	EvidenceTAS
GeneCALM3	AuthorityHGNC:1449	Mapping file id808 NCBI file	EvidenceTAS
GeneCAMKMT	AuthorityHGNC:26276	Mapping file id79823 NCBI file	EvidenceTAS
GeneEEF1A1	AuthorityHGNC:3189	Mapping file id1915 NCBI file	EvidenceTAS
GeneEEF1AKMT1	AuthorityHGNC:27351	Mapping file id221143 NCBI file	EvidenceTAS
GeneEEF1AKMT2	AuthorityHGNC:33787	Mapping file id399818 NCBI file	EvidenceTAS
GeneEEF2	AuthorityHGNC:3214	Mapping file id1938 NCBI file	EvidenceTAS
GeneEEF2KMT	AuthorityHGNC:32221	Mapping file id196483 NCBI file	EvidenceTAS
GeneETFB	AuthorityHGNC:3482	Mapping file id2109 NCBI file	EvidenceTAS
GeneETFBKMT	AuthorityHGNC:28739	Mapping file id254013 NCBI file	EvidenceTAS
GeneHSPA8	AuthorityHGNC:5241	Mapping file id3312 NCBI file	EvidenceTAS
GeneKIN	AuthorityHGNC:6327	Mapping file id22944 NCBI file	EvidenceTAS
GeneMETTL21A	AuthorityHGNC:30476	Mapping file id151194 NCBI file	EvidenceTAS
GeneMETTL22	AuthorityHGNC:28368	Mapping file id79091 NCBI file	EvidenceTAS
GenePRMT3	AuthorityHGNC:30163	Mapping file id10196 NCBI file	EvidenceTAS
GeneRPS2	AuthorityHGNC:10404	Mapping file id6187 NCBI file	EvidenceTAS
GeneVCP	AuthorityHGNC:12666	Mapping file id7415 NCBI file	EvidenceTAS
GeneVCPKMT	AuthorityHGNC:20352	Mapping file id79609 NCBI file	EvidenceTAS

Evidence codes on this page: TAS, as the mapping file writes them; a row carrying two was written twice by the file, once under each. The mapping file id says which file placed the gene: an NCBI Gene id from NCBI2Reactome_All_Levels.txt, an Ensembl gene id from Ensembl2Reactome_All_Levels.txt. The two files can disagree about a code, so a row's codes are the one file's view. Measured by this site's build over this release on 2026-09-09: of the 138,908 human pathway-gene pairs both files place, 207 (0.149 per cent, over 47 genes) carry TAS in the Ensembl file where the NCBI rows carry IEA alone.

Reactome mapping files, the human rows for this pathway · Reactome release 97 · read 2026-09-09 · Reactome downloads"NCBI2Reactome_All_Levels.txt" and "Ensembl2Reactome_All_Levels.txt", Reactome, release 97, https://reactome.org/download-data/ (date of access 2026-09-09). Data CC0; attribution encouraged.

03The hierarchy

Parents and children in this release's hierarchy

Parents

Post-translational protein modificationR-HSA-5975921,507 genes

Children

None: no pathway of this release's list names this one as a parent.

Reactome's hierarchy is a graph rather than a tree: a pathway can sit under more than one parent, and the gene counts are each pathway's own placements at every level under it, so a parent's count is not the sum of its children's.

Reactome, the human pathway list and hierarchy relationship file · Reactome release 97 · read 2026-09-09 · Reactome downloads"ReactomePathways.txt" and "ReactomePathwaysRelation.txt", Reactome, release 97, https://reactome.org/download-data/ (date of access 2026-09-09). Data CC0; attribution encouraged.