Pathway Human Homo sapiens

TRAF6 mediated NF-kB activation

R-HSA-933542 in Reactome release 97: under DDX58/IFIH1-mediated induction of interferon-alpha/beta, with 25 genes placed in it by the mapping files and 0 child pathways in the hierarchy.

The same number in the other species

Reactome writes the mouse and rat events it infers from a human pathway under the human number with the species token. A door opens only where that species' own list in this release holds the id: R-MMU-933542 (mouse), R-RNO-933542 (rat). Whether the event was inferred from this one is what the record says.

The record
The genes
Parents and children

Scripts are off

This page reads the pathway's record from its source while you read the rest; with scripts off the card stays at its loading line. Reload with scripts on to see it, or open the source's own page from the link in the card.

01The record

Reactome's own record of this pathway

What this tells you

The pathway list, the hierarchy and the genes on this page are read from the files Reactome publishes for Reactome release 97, built into this site on 2026-09-09: the pathway list, the hierarchy relationship file and the two gene mapping files. The record card is the one live read, Reactome's own record of this pathway.

Reactome's download page describes the mapping files: Mapping files link the source database identifier to the lowest level pathway diagram or subset of the pathway, all levels of the pathway hierarchy or database identifier to all reactions. [R13] The gene list here is the all-levels mapping of NCBI Gene ids, filled from the all-levels mapping of Ensembl ids where the NCBI file has no row for a gene; each row names which file it came from by the shape of its source id. Each row carries the evidence codes the file writes for it, as written and unranked, and both where the file writes both; no page of Reactome's documentation the survey read defines the codes, so this page does not expand them. A gene id the identity files do not name is kept as the source's own id with no door rather than turned into a symbol.

On citing what a search finds, Reactome says: It should be remembered that while we strive to contain the most current and accurate data, Reactome should not be used in citations where other primary sources of information are available. [R12] The record card prints the literature Reactome attaches to a curated pathway for that reason. The data are public domain: All data in the Reactome database and files derived from that data are licensed under the Creative Commons Public Domain Dedication (CC0). User may copy, modify, and distribute these data, even for commercial purposes, without asking for permission. Attribution is encouraged but not required. [R10] The pathway illustrations are licensed apart, under CC BY 4.0, and none is shown here.

A gene on this list is one Reactome places in this pathway in this release, and that is all the row says: it does not say the gene is expressed in a tissue or associated with a disease, which are the other two explorers' questions, read from other sources. Reactome's papers of record are [R01] [R02].

[R01] Ragueneau E, Gong C, Sinquin P, Sevilla C, Beavers D, Grentner A, et al. (2026). The Reactome Knowledgebase 2026. Nucleic Acids Research 54:D673-D681. PMID 41251150, doi 10.1093/nar/gkaf1223.
[R02] Milacic M, Beavers D, Conley P, Gong C, Gillespie M, Griss J, et al. (2024). The Reactome Pathway Knowledgebase 2024. Nucleic Acids Research 52:D672-D678. PMID 37941124, doi 10.1093/nar/gkad1025.
[R10] Reactome, reactome.org. License Agreement. https://reactome.org/license, read 2026-09-09.
[R12] Reactome, reactome.org. Citing us. https://reactome.org/cite, read 2026-09-09.
[R13] Reactome, reactome.org. Download. https://reactome.org/download-data, read 2026-09-09.

Stable id: R-HSA-933542.3
Class: Pathway
Species: Homo sapiens (human)
Inferred: no: a curated human pathway
Disease pathway: no
Released: 2010-12-14
Last updated: 2022-06-09
Events inside: 4, counted from the record

The TRAF6/TAK1 signal activates a canonical IKK complex, resulting in the activation of NF-kappaB as well as MAPK cascades leading to the activation of AP-1. Although TRAF6/TAK1 has been implicated in Tool like receptor (TLR) mediated cytokine production, the involvement of these molecules in the regulation of type I IFN induction mediated by RIG-I (DDX58)/MDA5 (IFIH1) pathway is largely unknown. According to the study done by Yoshida et al. the DDX58:MAVS pathway requires TRAF6 and MAP3K, MEKK1 to activate NF-kappaB and MAP Kinases for optimal induction of type I IFNs.

Literature Reactome attaches

TRAF6 and MEKK1 play a pivotal role in the RIG-I-like helicase antiviral pathwayJ Biol Chem, 2008, vol. 283, p. 36211-20PubMed 18984593

Reactome's record lists 7 orthologous events, R-RNO-933542 (Rattus norvegicus), R-MMU-933542 (Mus musculus) among the species this site serves, and 5 in species this site opens no page for.

Reactome ContentService, this pathway's record · Reactome v97 · read 2026-10-01 ET · Reactome pathway browser"TRAF6 mediated NF-kB activation". Reactome, release 97, https://reactome.org/PathwayBrowser/#/R-HSA-933542 with StableID: R-HSA-933542.3 (accessed 2026-10-01 ET). Reactome version 97 (CC0).

Reading this pathway's record from Reactome (the pathway record).Still reading. A first read of a pathway can take a while; this page waits up to 35 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.

02The genes

Genes Reactome places in this human pathway

The mapping files place 25 genes in this human pathway; showing 1 to 25, in pages of 100, sorted by symbol for reading. The order carries no ranking.

Genes Reactome places in this human pathway, page 1 of 1
Gene	Authority id	Mapping file id	Evidence codes
GeneAGER	AuthorityHGNC:320	Mapping file id177 NCBI file	EvidenceTAS
GeneAPP	AuthorityHGNC:620	Mapping file id351 NCBI file	EvidenceTAS
GeneCHUK	AuthorityHGNC:1974	Mapping file id1147 NCBI file	EvidenceTAS
GeneHMGB1	AuthorityHGNC:4983	Mapping file id3146 NCBI file	EvidenceTAS
GeneIFIH1	AuthorityHGNC:18873	Mapping file id64135 NCBI file	EvidenceIEA, TAS
GeneIKBKB	AuthorityHGNC:5960	Mapping file id3551 NCBI file	EvidenceTAS
GeneIKBKG	AuthorityHGNC:5961	Mapping file id8517 NCBI file	EvidenceTAS
GeneMAP3K1	AuthorityHGNC:6848	Mapping file id4214 NCBI file	EvidenceIEA, TAS
GeneMAVS	AuthorityHGNC:29233	Mapping file id57506 NCBI file	EvidenceIEA, TAS
GeneNFKB1	AuthorityHGNC:7794	Mapping file id4790 NCBI file	EvidenceTAS
GeneNFKB2	AuthorityHGNC:7795	Mapping file id4791 NCBI file	EvidenceTAS
GeneNFKBIA	AuthorityHGNC:7797	Mapping file id4792 NCBI file	EvidenceTAS
GeneNFKBIB	AuthorityHGNC:7798	Mapping file id4793 NCBI file	EvidenceTAS
GeneNKIRAS1	AuthorityHGNC:17899	Mapping file id28512 NCBI file	EvidenceTAS
GeneNKIRAS2	AuthorityHGNC:17898	Mapping file id28511 NCBI file	EvidenceTAS
GeneRELA	AuthorityHGNC:9955	Mapping file id5970 NCBI file	EvidenceTAS
GeneRIGI	AuthorityHGNC:19102	Mapping file id23586 NCBI file	EvidenceIEA, TAS
GeneRNF135	AuthorityHGNC:21158	Mapping file id84282 NCBI file	EvidenceIEA, TAS
GeneS100A12	AuthorityHGNC:10489	Mapping file id6283 NCBI file	EvidenceTAS
GeneS100B	AuthorityHGNC:10500	Mapping file id6285 NCBI file	EvidenceTAS
GeneSAA1	AuthorityHGNC:10513	Mapping file id6288 NCBI file	EvidenceTAS
GeneTRAF2	AuthorityHGNC:12032	Mapping file id7186 NCBI file	EvidenceIEA, TAS
GeneTRAF6	AuthorityHGNC:12036	Mapping file id7189 NCBI file	EvidenceIEA, TAS
GeneTRIM25	AuthorityHGNC:12932	Mapping file id7706 NCBI file	EvidenceIEA, TAS
GeneTRIM4	AuthorityHGNC:16275	Mapping file id89122 NCBI file	EvidenceIEA, TAS

Evidence codes on this page: IEA, TAS, as the mapping file writes them; a row carrying two was written twice by the file, once under each. The mapping file id says which file placed the gene: an NCBI Gene id from NCBI2Reactome_All_Levels.txt, an Ensembl gene id from Ensembl2Reactome_All_Levels.txt. The two files can disagree about a code, so a row's codes are the one file's view. Measured by this site's build over this release on 2026-09-09: of the 138,908 human pathway-gene pairs both files place, 207 (0.149 per cent, over 47 genes) carry TAS in the Ensembl file where the NCBI rows carry IEA alone.

Reactome mapping files, the human rows for this pathway · Reactome release 97 · read 2026-09-09 · Reactome downloads"NCBI2Reactome_All_Levels.txt" and "Ensembl2Reactome_All_Levels.txt", Reactome, release 97, https://reactome.org/download-data/ (date of access 2026-09-09). Data CC0; attribution encouraged.

03The hierarchy

Parents and children in this release's hierarchy

Parents

DDX58/IFIH1-mediated induction of interferon-alpha/betaR-HSA-16892885 genes

Children

None: no pathway of this release's list names this one as a parent.

Reactome's hierarchy is a graph rather than a tree: a pathway can sit under more than one parent, and the gene counts are each pathway's own placements at every level under it, so a parent's count is not the sum of its children's.

Reactome, the human pathway list and hierarchy relationship file · Reactome release 97 · read 2026-09-09 · Reactome downloads"ReactomePathways.txt" and "ReactomePathwaysRelation.txt", Reactome, release 97, https://reactome.org/download-data/ (date of access 2026-09-09). Data CC0; attribution encouraged.