Cytogenetic band 22q11.21NCBI: 22:19,941,772-19,969,975 on the plus strand, GRCh38.p14 (GCF_000001405.40), sequence NC_000022.11, annotation GCF_000001405.40-RS_2025_08 of 2025-08-01Coordinates are one-based with both ends included, as each source reports them.
Reading NCBI Gene and UniProt.Still reading. A first read of a gene can take a while; this page waits up to 30 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
02 / Transcripts and isoforms
The RNA a design targets
Ready in a moment
Reading NCBI Datasets and Ensembl.
03 / Expression by tissue
Where COMT is expressed
Ready in a moment
14 / Silencing this gene
From COMT to a sequence that silences it
The AUMsilence™ platform designs the sequences against the human COMT transcripts.
01
Choose the region
A knockdown oligonucleotide can sit in the 5' untranslated region, the coding sequence or the 3' untranslated region, and all three are used. The coding sequence and the 3' untranslated region are the usual first choices for an RNase H design; the 5' end near the start codon suits a steric block. The map above shows where each region sits on the isoforms it draws.
02
Cover the isoforms you mean
An exon every isoform carries silences the whole gene; an exon only some isoforms carry silences those and spares the rest. Decide which you want before a sequence is chosen, and check the reference transcript (MANE Select in human; RefSeq Select and Ensembl canonical in mouse and rat) is the one your cells express.
03
Think across species early
The orthologs panel says whether mouse and rat carry the same gene. Whether one oligonucleotide can serve two species is a sequence question, settled at design by matching the candidate against each transcript, not by the protein identity shown there.
04
Check expression in your model
A transcript that is not expressed in your cells cannot show knockdown. Confirm the gene is expressed in the cell type and condition you will use, from your own data or a reference atlas, before the order. The expression panel above gives GTEx's median per tissue for a human gene; for mouse and rat it says that no atlas is on this page yet.
04 / Protein
The protein COMT encodes
Ready in a moment
05 / Interactions
Proteins STRING associates with COMT
Ready in a moment
06 / Pathways
Where COMT acts, as Reactome curates it
Ready in a moment
07 / Disease associations
Diseases linked to COMT
Ready in a moment
08 / Variants
Classified variants of COMT
Ready in a moment
09 / Constraint
How much variation COMT tolerates
Ready in a moment
10 / Orthologs
The same gene in mouse, rat and human
Ready in a moment
11 /MicroRNAs
MicroRNAs hosted within COMT
Reading Ensembl and miRBase.
Ready in a moment
12 / Long non-coding RNAs
Long non-coding RNAs at the COMT locus
Reading Ensembl.
Ready in a moment
13 / Literature
Papers that mention COMT
Ready in a moment
05
Run the controls
A scramble control of the same chemistry, a positive control against a gene known to knock down in your cells, untreated cells, and a mock condition where a transfection reagent is used. Read knockdown at the RNA level first, then at the protein; the usage guide gives the timing and the concentrations to start from.
06
Pick the product
AUMsilence sdASO needs no transfection reagent and works in the cells that resist one. AUMsilence toASO is the transfection-optimised version of the same design, and AUMsiRNA™ is the siRNA route. The selection guide compares them.
Ensembl: 22:19,941,371-19,969,975 on the plus strand, GRCh38.p14 (GCA_000001405.29), release 116
Catechol-O-methyltransferase catalyzes the transfer of a methyl group from…
NCBI Gene summary
5 RefSeq and 42 Ensembl transcripts on GRCh38.p14; MANE Select NM_000754.4.
NCBI Datasets · Ensembl
54 GTEx tissues; the highest median in Nerve - Tibial, 52.7 TPM.
GTEx
271 residues, reviewed P21964; 8 entries from the member databases this page shows along the chain; mean pLDDT 93.94; 12 PDB entries.
UniProt · InterPro · AlphaFold DB · PDBe
The 25 highest-scoring STRING partners at or above a combined score of 0.4, of up to 25 asked for; MAOB, MAOA, CYP1A1 lead.
STRING v12.0
4 curated, 0 inferred Reactome pathways for P21964 in human, v98.
Reactome
610 Open Targets disease associations; Parkinson disease first, at 0.63.
Open Targets
ClinVar: 663 records for COMT, 395 pathogenic or likely pathogenic.
ClinVar
pLI 0.0006986 and LOEUF 1.09 in gnomAD v4 (GRCh38), on ENST00000361682.11.
gnomAD
Mouse Comt by 3 of 3 votes; rat Comt by 3 of 3 votes. RGD is not answering, so the ortholog list could not be shown. Try again later. Reference 1f8891d0-a1c.
Alliance · NCBI · Ensembl Compara
23,746 PubMed-indexed papers mention COMT at Europe PMC, newest first. Europe PMC ignores letter case, so the count also covers another species' symbol spelled with the same letters.
Europe PMC
Gene summary
Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.
Provided by RefSeq, Sep 2008, through NCBI Gene. NCBI disclaimer
Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol
NCBI Gene summary · NCBI Gene annotation RS_2025_08 · read · NCBI Gene 1312Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
UniProtKB function · 2026_04 · read · UniProt P21964UniProt data are available under the Creative Commons Attribution 4.0 licence.
Placed on GRCh38.p14 (GCF_000001405.40). MANE Select marks the one transcript RefSeq and Ensembl agree is the reference for this gene.
RefSeq 5 transcripts
coding sequence, tall
untranslated region, thin
non-coding exon
intron, fixed width
Genomic strand: plus. Drawn 5' to 3', so exon 1 sits at the left here and at the lowest coordinate on the chromosome.
NM_000754.4NM_000754.4MANE Select
NM_001362828.2NM_001362828.2
NM_001135161.2NM_001135161.2
NM_001135162.2NM_001135162.2
NM_007310.3NM_007310.3
scale
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
NCBI Datasets, RefSeq transcripts · NCBI Datasets 18.41.0; GCF_000001405.40-RS_2025_08 · read · NCBI Gene 1312Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
Ensembl 42 transcripts
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
Lengths are spliced lengths, as each source states them. Exon ranks follow the strand: on a minus-strand gene exon 1 has the highest genomic coordinate. Reference assembly for human: GRCh38.
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other, except 1 block too short to see, widened to a fixed few pixels; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
