Reading the record for E2F4 from HGNC, NCBI Gene and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 115 seconds for it, and its scripts then bring in the page, or a line saying what did not arrive.
The full name, the identifiers, the location and the notes from the sources arrive with the record. Nothing is filled in ahead of it.
Order door
The door to order for E2F4 opens with the record, which decides which product it carries. The order page itself is open now.
Cytogenetic band 16q22.1NCBI: 16:67,192,155-67,198,918 on the plus strand, GRCh38.p14 (GCF_000001405.40), sequence NC_000016.10, annotation GCF_000001405.40-RS_2025_08 of 2025-08-01Ensembl: 16:67,192,117-67,198,919 on the plus strand, GRCh38.p14 (GCA_000001405.29), release 116Coordinates are one-based with both ends included, as each source reports them.
The protein encoded by this gene is a member of the E2F family of…
NCBI Gene summary
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Reading NCBI Gene and UniProt.Still reading. A first read of a gene can take a while; this page waits up to 30 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
02 / Transcripts and isoforms
The RNA a design targets
1 RefSeq and 40 Ensembl transcripts on GRCh38.p14; MANE Select NM_001950.4.
NCBI Datasets · Ensembl
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03 / Expression by tissue
Where E2F4 is expressed
54 GTEx tissues; the highest median in Cells - EBV-transformed lymphocytes, 136 TPM.
GTEx
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04 / Protein
The protein E2F4 encodes
Reading UniProt, InterPro, AlphaFold DB and PDBe.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
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05 / Interactions
Proteins STRING associates with E2F4
The 25 highest-scoring STRING partners at or above a combined score of 0.4, of up to 25 asked for; TFDP2, SMAD3, RB1 lead.
STRING v12.0
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06 / Pathways
Where E2F4 acts, as Reactome curates it
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07 / Disease associations
Diseases linked to E2F4
361 Open Targets disease associations; neoplasm first, at 0.11.
Open Targets
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08 / Variants
Classified variants of E2F4
ClinVar: 103 records for E2F4, 24 pathogenic or likely pathogenic.
ClinVar
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09 / Constraint
How much variation E2F4 tolerates
pLI 0.8703 and LOEUF 0.547 in gnomAD v4 (GRCh38), on ENST00000379378.8.
gnomAD
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10 / Orthologs
The same gene in mouse, rat and human
Mouse E2f4 by 3 of 3 votes; rat E2f4 by 3 of 3 votes. RGD is not answering, so the ortholog list could not be shown. Try again later. Reference 6e8624f3-d91.
Alliance · NCBI · Ensembl Compara
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11 /MicroRNAs
MicroRNAs hosted within E2F4
No annotated microRNA lies within E2F4 in Ensembl release 116, on GRCh38.p14.
Ensembl · miRBase
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12 / Long non-coding RNAs
Long non-coding RNAs at the E2F4 locus
No annotated long non-coding RNA overlaps E2F4 in Ensembl release 116, on GRCh38.p14.
Ensembl
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13 / Literature
Papers that mention E2F4
Reading Europe PMC.Still reading. A first read of a gene can take a while; this page waits up to 55 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
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14 / Silencing this gene
From E2F4 to a sequence that silences it
The AUMsilence™ platform designs the sequences against the human transcripts on this page. Six decisions are yours before it does. What is written under each is AUM's guidance; the timings and the concentrations are in the usage guide below.
01
Choose the region
A knockdown oligonucleotide can sit in the 5' untranslated region, the coding sequence or the 3' untranslated region, and all three are used. The coding sequence and the 3' untranslated region are the usual first choices for an RNase H design; the 5' end near the start codon suits a steric block. The map above shows where each region sits on the isoforms it draws.
02
Cover the isoforms you mean
An exon every isoform carries silences the whole gene; an exon only some isoforms carry silences those and spares the rest. Decide which you want before a sequence is chosen, and check the reference transcript (MANE Select in human; RefSeq Select and Ensembl canonical in mouse and rat) is the one your cells express.
03
Think across species early
The orthologs panel says whether mouse and rat carry the same gene. Whether one oligonucleotide can serve two species is a sequence question, settled at design by matching the candidate against each transcript, not by the protein identity shown there.
04
Check expression in your model
A transcript that is not expressed in your cells cannot show knockdown. Confirm the gene is expressed in the cell type and condition you will use, from your own data or a reference atlas, before the order. The expression panel above gives GTEx's median per tissue for a human gene; for mouse and rat it says that no atlas is on this page yet.
05
Run the controls
A scramble control of the same chemistry, a positive control against a gene known to knock down in your cells, untreated cells, and a mock condition where a transfection reagent is used. Read knockdown at the RNA level first, then at the protein; the usage guide gives the timing and the concentrations to start from.
06
Pick the product
AUMsilence sdASO needs no transfection reagent and works in the cells that resist one. AUMsilence toASO is the transfection-optimised version of the same design, and AUMsiRNA™ is the siRNA route. The selection guide compares them.
For research use only. Not for use in diagnostic or therapeutic procedures.
Gene summary
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.
Provided by RefSeq, Jul 2008, through NCBI Gene. NCBI disclaimer
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis
NCBI Gene summary · NCBI Gene annotation RS_2025_08 · read · NCBI Gene 1874Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
UniProtKB function · 2026_03 · read · UniProt Q16254UniProt data are available under the Creative Commons Attribution 4.0 licence.
Placed on GRCh38.p14 (GCF_000001405.40). MANE Select marks the one transcript RefSeq and Ensembl agree is the reference for this gene.
RefSeq 1 transcript
coding sequence, tall
untranslated region, thin
non-coding exon
intron, fixed width
Genomic strand: plus. Drawn 5' to 3', so exon 1 sits at the left here and at the lowest coordinate on the chromosome.
NM_001950.4NM_001950.4MANE Select
scale
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
Lengths are spliced lengths, as each source states them. Exon ranks follow the strand: on a minus-strand gene exon 1 has the highest genomic coordinate. Reference assembly for human: GRCh38.
10 curated, 0 inferred Reactome pathways for Q16254 in human, v97.
Reactome
413 residues, reviewed Q16254; 14 entries from the member databases this page shows along the chain; mean pLDDT 64.19; 2 PDB entries.
UniProt · InterPro · AlphaFold DB · PDBe
5,211 PubMed-indexed papers mention E2F4 at Europe PMC, newest first. Europe PMC ignores letter case, so the count also covers another species' symbol spelled with the same letters.
