Reading the record for FBXL5 from HGNC, NCBI Gene and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 115 seconds for it, and its scripts then bring in the page, or a line saying what did not arrive.
The full name, the identifiers, the location and the notes from the sources arrive with the record. Nothing is filled in ahead of it.
Order door
The door to order for FBXL5 opens with the record, which decides which product it carries. The order page itself is open now.
Cytogenetic band 4p15.32NCBI: 4:15,604,381-15,681,569 on the minus strand, GRCh38.p14 (GCF_000001405.40), sequence NC_000004.12, annotation GCF_000001405.40-RS_2025_08 of 2025-08-01Ensembl: 4:15,569,541-15,681,679 on the minus strand, GRCh38.p14 (GCA_000001405.29), release 116Coordinates are one-based with both ends included, as each source reports them.
This gene encodes a member of the F-box protein family which is…
NCBI Gene summary
Ready in a moment
Reading NCBI Gene and UniProt.Still reading. A first read of a gene can take a while; this page waits up to 30 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
02 / Transcripts and isoforms
The RNA a design targets
Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
03 / Expression by tissue
Where FBXL5 is expressed
Reading GTEx and the Human Protein Atlas.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
04 / Protein
The protein FBXL5 encodes
Reading UniProt, InterPro, AlphaFold DB and PDBe.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
05 / Interactions
Proteins STRING associates with FBXL5
The 25 highest-scoring STRING partners at or above a combined score of 0.4, of up to 25 asked for; SKP1, CUL1, IREB2 lead.
STRING v12.0
Ready in a moment
06 / Pathways
Where FBXL5 acts, as Reactome curates it
Reading UniProt and Reactome.Still reading. A first read of a gene can take a while; this page waits up to 60 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
07 / Disease associations
Diseases linked to FBXL5
201 Open Targets disease associations; Joubert syndrome 9 first, at 0.57.
Open Targets
Ready in a moment
08 / Variants
Classified variants of FBXL5
Reading ClinVar.Still reading. A first read of a gene can take a while; this page waits up to 60 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
09 / Constraint
How much variation FBXL5 tolerates
Reading gnomAD and Open Targets.Still reading. A first read of a gene can take a while; this page waits up to 45 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
10 / Orthologs
The same gene in mouse, rat and human
Reading the Alliance, NCBI, Ensembl Compara and RGD.Still reading. A first read of a gene can take a while; this page waits up to 145 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
11 /MicroRNAs
MicroRNAs hosted within FBXL5
Reading Ensembl and miRBase.Still reading. A first read of a gene can take a while; this page waits up to 110 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
12 / Long non-coding RNAs
Long non-coding RNAs at the FBXL5 locus
Reading Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 110 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
13 / Literature
Papers that mention FBXL5
Reading Europe PMC.Still reading. A first read of a gene can take a while; this page waits up to 55 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
14 / Silencing this gene
From FBXL5 to a sequence that silences it
The AUMsilence™ platform designs the sequences against the human transcripts on this page. Six decisions are yours before it does. What is written under each is AUM's guidance; the timings and the concentrations are in the usage guide below.
01
Choose the region
A knockdown oligonucleotide can sit in the 5' untranslated region, the coding sequence or the 3' untranslated region, and all three are used. The coding sequence and the 3' untranslated region are the usual first choices for an RNase H design; the 5' end near the start codon suits a steric block. The map above shows where each region sits on the isoforms it draws.
02
Cover the isoforms you mean
An exon every isoform carries silences the whole gene; an exon only some isoforms carry silences those and spares the rest. Decide which you want before a sequence is chosen, and check the reference transcript (MANE Select in human; RefSeq Select and Ensembl canonical in mouse and rat) is the one your cells express.
03
Think across species early
The orthologs panel says whether mouse and rat carry the same gene. Whether one oligonucleotide can serve two species is a sequence question, settled at design by matching the candidate against each transcript, not by the protein identity shown there.
04
Check expression in your model
A transcript that is not expressed in your cells cannot show knockdown. Confirm the gene is expressed in the cell type and condition you will use, from your own data or a reference atlas, before the order. The expression panel above gives GTEx's median per tissue for a human gene; for mouse and rat it says that no atlas is on this page yet.
05
Run the controls
A scramble control of the same chemistry, a positive control against a gene known to knock down in your cells, untreated cells, and a mock condition where a transfection reagent is used. Read knockdown at the RNA level first, then at the protein; the usage guide gives the timing and the concentrations to start from.
06
Pick the product
AUMsilence sdASO needs no transfection reagent and works in the cells that resist one. AUMsilence toASO is the transfection-optimised version of the same design, and AUMsiRNA™ is the siRNA route. The selection guide compares them.
For research use only. Not for use in diagnostic or therapeutic procedures.
Gene summary
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats. Alternatively spliced transcript variants have been described for this locus.
Provided by RefSeq, Aug 2010, through NCBI Gene. NCBI disclaimer
Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2 (PubMed:19762596, PubMed:19762597). The C-terminal domain of FBXL5 contains a redox-sensitive [2Fe-2S] cluster that, upon oxidation, promotes binding to IRP2 to effect its oxygen-dependent degradation (PubMed:32126207). Under iron deficiency conditions, the N-terminal hemerythrin-like (Hr) region, which contains a diiron metal center, cannot bind iron and undergoes conformational changes that destabilize the FBXL5 protein and cause its ubiquitination and degradation (PubMed:19762596, PubMed:19762597). When intracellular iron levels start rising, the Hr region is stabilized (PubMed:19762596, PubMed:19762597). Additional increases in iron levels facilitate the assembly and incorporation of a redox active [2Fe-2S] cluster in the C-terminal domain (PubMed:32126207). Only when oxygen level is high enough to maintain the cluster in its oxidized state can FBXL5 recruit IRP2 as a substrate for polyubiquitination and degradation (PubMed:32126207). Promotes ubiquitination and subsequent degradation of the dynactin complex component DCTN1 (PubMed:17532294). Within the nucleus, promotes the ubiquitination of SNAI1; preventing its interaction with DNA and promoting its degradation (PubMed:24157836). Negatively regulates DNA damage response by mediating the ubiquitin-proteasome degradation of the DNA repair protein NABP2 (PubMed:25249620)
NCBI Gene summary · NCBI Gene annotation RS_2025_08 · read · NCBI Gene 26234Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
UniProtKB function · 2026_03 · read · UniProt Q9UKA1UniProt data are available under the Creative Commons Attribution 4.0 licence.
pLI 0.9985 and LOEUF 0.461 in gnomAD v4 (GRCh38), on ENST00000341285.8.
gnomAD
54 GTEx tissues; the highest median in Whole Blood, 143 TPM.
GTEx
No annotated microRNA lies within FBXL5 in Ensembl release 116, on GRCh38.p14.
Ensembl · miRBase
2 long non-coding RNA genes overlap FBXL5 in Ensembl release 116, 1 antisense, none with a symbol.
867 PubMed-indexed papers mention FBXL5 at Europe PMC, newest first. Europe PMC ignores letter case, so the count also covers another species' symbol spelled with the same letters.
Europe PMC
4 curated, 0 inferred Reactome pathways for Q9UKA1 in human, v97.
Reactome
Mouse Fbxl5 by 3 of 3 votes; rat Fbxl5 by 3 of 3 votes. RGD is not answering, so the ortholog list could not be shown. Try again later. Reference 86ce122b-f7e.
Alliance · NCBI · Ensembl Compara
23 RefSeq and 42 Ensembl transcripts on GRCh38.p14; MANE Select NM_012161.4. 15 RefSeq transcripts of FBXL5 carry no exon placement.
NCBI Datasets · Ensembl
Placed on GRCh38.p14 (GCF_000001405.40). MANE Select marks the one transcript RefSeq and Ensembl agree is the reference for this gene.
RefSeq 38 transcripts
coding sequence, tall
untranslated region, thin
non-coding exon
intron, fixed width
Genomic strand: minus. Drawn 5' to 3', so exon 1 sits at the left here and at the highest coordinate on the chromosome.
NM_012161.4NM_012161.4MANE Select
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
NCBI's product report carries no genomic placement for this transcript, so its exon coordinates are not available from NCBI
Ready in a moment
Not listed, because the source places them on another assembly only: XM_054349652.1 (not placed on GRCh38.p14); XM_054349653.1 (not placed on GRCh38.p14); XM_054349654.1 (not placed on GRCh38.p14); XM_054349655.1 (not placed on GRCh38.p14); XM_054349656.1 (not placed on GRCh38.p14); XM_054349657.1 (not placed on GRCh38.p14); XM_054349658.1 (not placed on GRCh38.p14); XM_054349659.1 (not placed on GRCh38.p14); XM_054349660.1 (not placed on GRCh38.p14); XM_054349661.1 (not placed on GRCh38.p14); XM_054349662.1 (not placed on GRCh38.p14); XM_054349663.1 (not placed on GRCh38.p14); XM_054349664.1 (not placed on GRCh38.p14); XM_054349665.1 (not placed on GRCh38.p14); XM_054349666.1 (not placed on GRCh38.p14); XM_054349667.1 (not placed on GRCh38.p14); XM_054349668.1 (not placed on GRCh38.p14); XM_054349669.1 (not placed on GRCh38.p14); XM_054349670.1 (not placed on GRCh38.p14); XM_054349671.1 (not placed on GRCh38.p14); XM_054349672.1 (not placed on GRCh38.p14); XR_007074495.1 (not placed on GRCh38.p14).
NCBI Datasets, RefSeq transcripts · NCBI Datasets 18.38.0; GCF_000001405.40-RS_2025_08 · read · NCBI Gene 26234Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
Ensembl 42 transcripts
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
Lengths are spliced lengths, as each source states them. Exon ranks follow the strand: on a minus-strand gene exon 1 has the highest genomic coordinate. Reference assembly for human: GRCh38.
XM_047450052.1XM_047450052.1
XM_047450051.1XM_047450051.1
XM_047450060.1XM_047450060.1
XM_047450050.1XM_047450050.1
XM_047450061.1XM_047450061.1
XM_017008019.3XM_017008019.3
XM_006713959.4XM_006713959.4
XM_047450049.1XM_047450049.1
XM_047450053.1XM_047450053.1
XM_047450054.1XM_047450054.1
XM_047450057.1XM_047450057.1
XM_047450058.1XM_047450058.1
XM_011513831.3XM_011513831.3
XM_047450055.1XM_047450055.1
XM_047450056.1XM_047450056.1
XM_047450059.1XM_047450059.1
NM_001193534.2NM_001193534.2
NM_001193535.2NM_001193535.2
XM_011513833.3XM_011513833.3
NR_036464.2NR_036464.2
XR_007058062.1XR_007058062.1
XR_007058061.1XR_007058061.1
scale
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it. Not drawn, because the source gave no placement: NM_001443848.1, NM_001443845.1, NM_001443842.1, NM_001443841.1, NM_001443843.1, NM_001443844.1, NM_001443839.1, NM_001443840.1, NM_001446240.1, NM_001446241.1, NM_001443846.1, NM_001446242.1, NM_001446238.1, NM_001446239.1, NM_001443847.1.
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
