Reading the record for GNAS from HGNC, NCBI Gene and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 115 seconds for it, and its scripts then bring in the page, or a line saying what did not arrive.
The full name, the identifiers, the location and the notes from the sources arrive with the record. Nothing is filled in ahead of it.
Order door
The door to order for GNAS opens with the record, which decides which product it carries. The order page itself is open now.
Cytogenetic band 20q13.32NCBI: 20:58,839,748-58,911,192 on the plus strand, GRCh38.p14 (GCF_000001405.40), sequence NC_000020.11, annotation GCF_000001405.40-RS_2025_08 of 2025-08-01Ensembl: no location was read.Coordinates are one-based with both ends included, as each source reports them.
Ensembl did not answer (upstream error); the Ensembl location and release are missing
more than one UniProt entry is listed for this gene (O95467, P63092, P84996, Q5JWF2); none is chosen for the function summary, which shows each of them
Silence this gene
The order page opens with GNAS and human filled in. The sequences are designed against the transcripts below; the price is on that page.
This locus has a highly complex imprinted expression pattern. UniProt: 4 entries (O95467, P63092, P84996, Q5JWF2), none chosen.
NCBI Gene summary · UniProtKB
Ready in a moment
Reading NCBI Gene and UniProt.Still reading. A first read of a gene can take a while; this page waits up to 30 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
02 / Transcripts and isoforms
The RNA a design targets
37 RefSeq transcripts on GRCh38.p14; MANE Select NM_000516.7. Ensembl is not answering, so the Ensembl transcripts could not be shown. Try again later. Reference 883bfcac-bb5.
NCBI Datasets
Ready in a moment
Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
03 / Expression by tissue
Where GNAS is expressed
54 GTEx tissues; the highest median in Pituitary, 1,184 TPM.
GTEx
Ready in a moment
04 / Protein
The protein GNAS encodes
GNAS is linked to 4 UniProt accessions and none was chosen; the candidates are listed
UniProt
Ready in a moment
05 / Interactions
Proteins STRING associates with GNAS
The 25 highest-scoring STRING partners at or above a combined score of 0.4, of up to 25 asked for; GNB1, GNG2, PTH1R lead.
STRING v12.0
Ready in a moment
06 / Pathways
Where GNAS acts, as Reactome curates it
GNAS is linked to 4 UniProt accessions and none was chosen; the candidates are listed
UniProt
Ready in a moment
07 / Disease associations
Diseases linked to GNAS
1,167 Open Targets disease associations; pseudohypoparathyroidism type 1A first, at 0.84.
Open Targets
Ready in a moment
08 / Variants
Classified variants of GNAS
ClinVar: 1,433 records for GNAS, 284 pathogenic or likely pathogenic.
ClinVar
Ready in a moment
09 / Constraint
How much variation GNAS tolerates
pLI above 0.9999 and LOEUF 0.265 in gnomAD v4 (GRCh38), on ENST00000371085.8.
gnomAD
Ready in a moment
10 / Orthologs
The same gene in mouse, rat and human
Mouse Gnas by 2 of 2 votes; rat Gnas by 2 of 2 votes. RGD is not answering, so the ortholog list could not be shown. Try again later. Reference cb540ff0-7ed.
Alliance · NCBI
Ready in a moment
11 /MicroRNAs
MicroRNAs hosted within GNAS
Ensembl is not answering, so the non-coding annotation could not be shown. Try again later. Reference 862ab2ee-b97.
Ready in a moment
12 / Long non-coding RNAs
Long non-coding RNAs at the GNAS locus
Ensembl is not answering, so the non-coding annotation could not be shown. Try again later. Reference 862ab2ee-b97.
Ready in a moment
13 / Literature
Papers that mention GNAS
11,278 PubMed-indexed papers mention GNAS at Europe PMC, newest first. Europe PMC ignores letter case, so the count also covers another species' symbol spelled with the same letters.
Europe PMC
Ready in a moment
14 / Silencing this gene
From GNAS to a sequence that silences it
The AUMsilence™ platform designs the sequences against the human transcripts on this page. Six decisions are yours before it does. What is written under each is AUM's guidance; the timings and the concentrations are in the usage guide below.
01
Choose the region
A knockdown oligonucleotide can sit in the 5' untranslated region, the coding sequence or the 3' untranslated region, and all three are used. The coding sequence and the 3' untranslated region are the usual first choices for an RNase H design; the 5' end near the start codon suits a steric block. The map above shows where each region sits on the isoforms it draws.
02
Cover the isoforms you mean
An exon every isoform carries silences the whole gene; an exon only some isoforms carry silences those and spares the rest. Decide which you want before a sequence is chosen, and check the reference transcript (MANE Select in human; RefSeq Select and Ensembl canonical in mouse and rat) is the one your cells express.
03
Think across species early
The orthologs panel says whether mouse and rat carry the same gene. Whether one oligonucleotide can serve two species is a sequence question, settled at design by matching the candidate against each transcript, not by the protein identity shown there.
04
Check expression in your model
A transcript that is not expressed in your cells cannot show knockdown. Confirm the gene is expressed in the cell type and condition you will use, from your own data or a reference atlas, before the order. The expression panel above gives GTEx's median per tissue for a human gene; for mouse and rat it says that no atlas is on this page yet.
05
Run the controls
A scramble control of the same chemistry, a positive control against a gene known to knock down in your cells, untreated cells, and a mock condition where a transfection reagent is used. Read knockdown at the RNA level first, then at the protein; the usage guide gives the timing and the concentrations to start from.
06
Pick the product
AUMsilence sdASO needs no transfection reagent and works in the cells that resist one. AUMsilence toASO is the transfection-optimised version of the same design, and AUMsiRNA™ is the siRNA route. The selection guide compares them.
For research use only. Not for use in diagnostic or therapeutic procedures.
Gene summary
This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
Provided by RefSeq, Aug 2012, through NCBI Gene. NCBI disclaimer
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, P63092
Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs) (PubMed:12391161, PubMed:17110384, PubMed:21488135, PubMed:26206488, PubMed:8702665, PubMed:10200251). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed:12391161, PubMed:17110384, PubMed:10200251). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed:12391161, PubMed:17110384, PubMed:10200251). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed:12391161, PubMed:17110384, PubMed:10200251). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:12391161, PubMed:17110384, PubMed:10200251). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP (PubMed:17110384, PubMed:26206488, PubMed:26206488, PubMed:8702665). Functions downstream of beta-adrenergic receptors (PubMed:21488135). Stimulates the Ras signaling pathway via RAPGEF2 (PubMed:12391161)
May inhibit the adenylyl cyclase-stimulating activity of guanine nucleotide-binding protein G(s) subunit alpha which is produced from the same locus in a different open reading frame
Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas, Q5JWF2
Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP. GNAS functions downstream of several GPCRs, including beta-adrenergic receptors. XLas isoforms interact with the same set of receptors as Gnas isoforms
Notes on the UniProt entries
this gene is linked to 4 reviewed UniProt entries, each a protein of its own; none is chosen
NCBI Gene summary · NCBI Gene annotation RS_2025_08 · read · NCBI Gene 2778Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
UniProtKB function · 2026_03 · read · UniProt O95467UniProt data are available under the Creative Commons Attribution 4.0 licence.
UniProtKB function · 2026_03 · read · UniProt P63092UniProt data are available under the Creative Commons Attribution 4.0 licence.
UniProtKB function · 2026_03 · read · UniProt P84996UniProt data are available under the Creative Commons Attribution 4.0 licence.
UniProtKB function · 2026_03 · read · UniProt Q5JWF2UniProt data are available under the Creative Commons Attribution 4.0 licence.
Placed on GRCh38.p14 (GCF_000001405.40). MANE Select marks the one transcript RefSeq and Ensembl agree is the reference for this gene.
RefSeq 37 transcripts
coding sequence, tall
untranslated region, thin
non-coding exon
intron, fixed width
Genomic strand: plus. Drawn 5' to 3', so exon 1 sits at the left here and at the lowest coordinate on the chromosome.
NM_000516.7NM_000516.7MANE Select
NM_080425.4NM_080425.4MANE Plus Clinical
NM_016592.5NM_016592.5MANE Plus Clinical
XM_017027812.3XM_017027812.3
NM_001077490.3NM_001077490.3
XM_017027813.3XM_017027813.3
NM_001410913.1NM_001410913.1
NM_001309883.1NM_001309883.1
XM_047440113.1XM_047440113.1
NM_001410912.1NM_001410912.1
XM_017027815.2XM_017027815.2
NM_001077488.5NM_001077488.5
NM_080426.4NM_080426.4
NM_001077489.4NM_001077489.4
XM_047440116.1XM_047440116.1
XM_047440121.1XM_047440121.1
XM_047440123.1XM_047440123.1
XM_047440114.1XM_047440114.1
NM_001309861.2NM_001309861.2
XM_047440122.1XM_047440122.1
XM_047440125.1XM_047440125.1
NM_001309840.2NM_001309840.2
XM_047440117.1XM_047440117.1
NM_001439291.1NM_001439291.1
XM_047440118.1XM_047440118.1
NM_001438273.1NM_001438273.1
XM_047440120.1XM_047440120.1
NM_001438274.1NM_001438274.1
XM_047440115.1XM_047440115.1
NM_001438276.1NM_001438276.1
XM_017027820.1XM_017027820.1
XM_047440124.1XM_047440124.1
XM_047440119.1XM_047440119.1
NM_001438275.1NM_001438275.1
NM_001309842.2NM_001309842.2
NR_132273.1NR_132273.1
NR_132272.2NR_132272.2
scale
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other, except 1 block too short to see, widened to a fixed few pixels; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
Not listed, because the source places them on another assembly only: XM_054323365.1 (not placed on GRCh38.p14); XM_054323366.1 (not placed on GRCh38.p14); XM_054323367.1 (not placed on GRCh38.p14); XM_054323368.1 (not placed on GRCh38.p14); XM_054323370.1 (not placed on GRCh38.p14); XM_054323371.1 (not placed on GRCh38.p14); XM_054323372.1 (not placed on GRCh38.p14); XM_054323373.1 (not placed on GRCh38.p14); XM_054323375.1 (not placed on GRCh38.p14); XM_054323377.1 (not placed on GRCh38.p14); XM_054323378.1 (not placed on GRCh38.p14).
NCBI Datasets, RefSeq transcripts · NCBI Datasets 18.37.0; GCF_000001405.40-RS_2025_08 · read · NCBI Gene 2778Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
Ensembl 0 transcripts
Ensembl is not answering, so the Ensembl transcripts could not be shown. Try again later.
Reference 883bfcac-bb5
Lengths are spliced lengths, as each source states them. Exon ranks follow the strand: on a minus-strand gene exon 1 has the highest genomic coordinate. Reference assembly for human: GRCh38.
