Gene Human Homo sapiens

IRS4

insulin receptor substrate 4

gene with protein product

Human IRS4MouseRat

Reading the ortholog votes for mouse and rat.

Location: Cytogenetic band Xq22.3NCBI: X:108,719,946-108,736,563 on the minus strand, GRCh38.p14 (GCF_000001405.40), sequence NC_000023.11, annotation GCF_000001405.40-RS_2025_08 of 2025-08-01Ensembl: X:108,719,946-108,736,563 on the minus strand, GRCh38.p14 (GCA_000001405.29), release 116Coordinates are one-based with both ends included, as each source reports them.
Also known as: PY160, IRS-4

Gene identity, from HGNC · HGNC 2026-10-02 · read 2026-10-03 ET · HGNC:6128

Silence this gene

The order page opens with IRS4 and human filled in. The sequences are designed against the transcripts below; the price is on that page.

Order for IRS4

sdASO, toASO or siRNA

Bring your own sequence Transcripts

For research use only. Not for use in diagnostic or therapeutic procedures.

Summary
Transcripts
Expression
Protein
Interactions
Pathways
Disease
Variants
Constraint
Orthologs
MicroRNAs
Long non-coding RNAs
Literature
Silencing this gene

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01 Summary and function

What IRS4 does

IRS4 encodes the insulin receptor substrate 4, a cytoplasmic protein that…

NCBI Gene summary

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02 Transcripts and isoforms

The RNA a design targets

Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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03 Expression by tissue

Where IRS4 is expressed

Reading GTEx and the Human Protein Atlas.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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04 Protein

The protein IRS4 encodes

Reading UniProt, InterPro, AlphaFold DB and PDBe.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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05 Interactions

Proteins STRING associates with IRS4

Reading STRING.Still reading. A first read of a gene can take a while; this page waits up to 50 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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06 Pathways

Where IRS4 acts, as Reactome curates it

Reading UniProt and Reactome.Still reading. A first read of a gene can take a while; this page waits up to 60 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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07 Disease associations

Diseases linked to IRS4

Reading Open Targets and ClinGen.Still reading. A first read of a gene can take a while; this page waits up to 105 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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08 Variants

Classified variants of IRS4

Reading ClinVar.Still reading. A first read of a gene can take a while; this page waits up to 60 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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09 Constraint

How much variation IRS4 tolerates

pLI 0.9945 and LOEUF 0.475 in gnomAD v4 (GRCh38), on ENST00000372129.4.

gnomAD

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10 Orthologs

The same gene in mouse, rat and human

Reading the Alliance, NCBI, Ensembl Compara and RGD.Still reading. A first read of a gene can take a while; this page waits up to 145 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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11 MicroRNAs

MicroRNAs hosted within IRS4

Reading Ensembl and miRBase.Still reading. A first read of a gene can take a while; this page waits up to 110 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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12 Long non-coding RNAs

Long non-coding RNAs at the IRS4 locus

Reading Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 110 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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13 Literature

Papers that mention IRS4

Reading Europe PMC.Still reading. A first read of a gene can take a while; this page waits up to 55 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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14 Silencing this gene

From IRS4 to a sequence that silences it

The AUMsilence platform designs the sequences against the human transcripts on this page. Six decisions are yours before it does. What is written under each is AUM's guidance; the timings and the concentrations are in the usage guide below.

01
Choose the region
A knockdown oligonucleotide can sit in the 5' untranslated region, the coding sequence or the 3' untranslated region, and all three are used. The coding sequence and the 3' untranslated region are the usual first choices for an RNase H design; the 5' end near the start codon suits a steric block. The map above shows where each region sits on the isoforms it draws.
02
Cover the isoforms you mean
An exon every isoform carries silences the whole gene; an exon only some isoforms carry silences those and spares the rest. Decide which you want before a sequence is chosen, and check the reference transcript (MANE Select in human; RefSeq Select and Ensembl canonical in mouse and rat) is the one your cells express.
03
Think across species early
The orthologs panel says whether mouse and rat carry the same gene. Whether one oligonucleotide can serve two species is a sequence question, settled at design by matching the candidate against each transcript, not by the protein identity shown there.
04
Check expression in your model
A transcript that is not expressed in your cells cannot show knockdown. Confirm the gene is expressed in the cell type and condition you will use, from your own data or a reference atlas, before the order. The expression panel above gives GTEx's median per tissue for a human gene; for mouse and rat it says that no atlas is on this page yet.
05
Run the controls
A scramble control of the same chemistry, a positive control against a gene known to knock down in your cells, untreated cells, and a mock condition where a transfection reagent is used. Read knockdown at the RNA level first, then at the protein; the usage guide gives the timing and the concentrations to start from.
06
Pick the product
AUMsilence sdASO needs no transfection reagent and works in the cells that resist one. AUMsilence toASO is the transfection-optimised version of the same design, and AUMsiRNA is the siRNA route. The selection guide compares them.

Order for IRS4 Bring your own sequence The usage guide Selection guide

For research use only. Not for use in diagnostic or therapeutic procedures.

Gene Human Homo sapiens

IRS4

Reading the record for IRS4 from HGNC, NCBI Gene and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 115 seconds for it, and its scripts then bring in the page, or a line saying what did not arrive.

The full name, the identifiers, the location and the notes from the sources arrive with the record. Nothing is filled in ahead of it.

Order door

The door to order for IRS4 opens with the record, which decides which product it carries. The order page itself is open now.

Bring your own sequence

For research use only. Not for use in diagnostic or therapeutic procedures.

This page is still reading the record. Its scripts replace this shell with the record when it arrives; with scripts off, it stays at this shell however long you wait.

01 Summary and function

What IRS4 does

Waiting for the record before reading NCBI Gene and UniProt.

02 Transcripts and isoforms

The RNA a design targets

Waiting for the record before reading NCBI Datasets and Ensembl.

03 Expression by tissue

Where IRS4 is expressed

Waiting for the record before reading GTEx and the Human Protein Atlas.

04 Protein

The protein IRS4 encodes

Waiting for the record before reading UniProt, InterPro, AlphaFold DB and PDBe.

05 Interactions

Proteins STRING associates with IRS4

Waiting for the record before reading STRING.

06 Pathways

Where IRS4 acts, as Reactome curates it

Waiting for the record before reading UniProt and Reactome.

07 Disease associations

Diseases linked to IRS4

Waiting for the record before reading Open Targets and ClinGen.

08 Variants

Classified variants of IRS4

Waiting for the record before reading ClinVar.

09 Constraint

How much variation IRS4 tolerates

Waiting for the record before reading gnomAD and Open Targets.

10 Orthologs

The same gene in mouse, rat and human

Waiting for the record before reading the Alliance, NCBI, Ensembl Compara and RGD.

11 MicroRNAs

MicroRNAs hosted within IRS4

Waiting for the record before reading Ensembl and miRBase.

12 Long non-coding RNAs

Long non-coding RNAs at the IRS4 locus

Waiting for the record before reading Ensembl.

13 Literature

Papers that mention IRS4

Waiting for the record before reading Europe PMC.

Accession	Name	Biotype	Labels	Exons	Spliced length	Protein	Sequence
NM_001379150.1matches ENST00000372129.4	transcript variant 2	protein coding	MANE Select	2	6,563 nt	1,256 aaNP_001366079.1	Ready in a moment
XM_006724713.4	transcript variant X2	protein coding model	none	2	15,937 nt	1,256 aaXP_006724776.1	Ready in a moment
NM_001440817.1	transcript variant 3	protein coding	none	3	6,589 nt	1,300 aaNP_001427746.1	Ready in a moment
NM_003604.2matches ENST00000564206.2	transcript variant 1	Ready in a moment

Accession	Name	Biotype	Labels	Exons	Spliced length	Protein	Sequence
NM_001379150.1matches ENST00000372129.4	transcript variant 2	protein coding	MANE Select	2	6,563 nt	1,256 aaNP_001366079.1	Ready in a moment
XM_006724713.4	transcript variant X2	protein coding model	none	2	15,937 nt	1,256 aaXP_006724776.1	Ready in a moment
NM_001440817.1	transcript variant 3	protein coding	none	3	6,589 nt	1,300 aaNP_001427746.1	Ready in a moment
NM_003604.2matches ENST00000564206.2	transcript variant 1	Ready in a moment

Accession	Name	Biotype	Labels	Exons	Spliced length	Protein	Sequence
ENST00000372129.4matches NM_001379150.1	IRS4-201	protein coding	MANE SelectEnsembl canonical	2	6,563 nt	1,256 aaENSP00000361202.3	Ready in a moment
ENST00000564206.2	IRS4-202	protein coding	none	1	16,461 nt	1,257 aaENSP00000505547.1	Ready in a moment

IRS4

What IRS4 does

The RNA a design targets

Where IRS4 is expressed

The protein IRS4 encodes

Proteins STRING associates with IRS4

Where IRS4 acts, as Reactome curates it

Diseases linked to IRS4

Classified variants of IRS4

How much variation IRS4 tolerates

The same gene in mouse, rat and human

MicroRNAs hosted within IRS4

Long non-coding RNAs at the IRS4 locus

Papers that mention IRS4

From IRS4 to a sequence that silences it

Choose the region

Cover the isoforms you mean

Think across species early

Check expression in your model

Run the controls

Pick the product

IRS4

What IRS4 does

The RNA a design targets

Where IRS4 is expressed

The protein IRS4 encodes

Proteins STRING associates with IRS4

Where IRS4 acts, as Reactome curates it

Diseases linked to IRS4

Classified variants of IRS4

How much variation IRS4 tolerates

The same gene in mouse, rat and human

MicroRNAs hosted within IRS4

Long non-coding RNAs at the IRS4 locus

Papers that mention IRS4

IRS4

What IRS4 does

Gene summary

Protein function

The RNA a design targets

RefSeq 4 transcripts

A window on one transcript

Ensembl 2 transcripts

A window on one transcript

Where IRS4 is expressed

The protein IRS4 encodes

Proteins STRING associates with IRS4

Where IRS4 acts, as Reactome curates it

Diseases linked to IRS4

Classified variants of IRS4

How much variation IRS4 tolerates

The same gene in mouse, rat and human

MicroRNAs hosted within IRS4

Long non-coding RNAs at the IRS4 locus

Papers that mention IRS4

From IRS4 to a sequence that silences it

Choose the region

Cover the isoforms you mean

Think across species early

Check expression in your model

Run the controls

Pick the product

Gene summary

Protein function

RefSeq 4 transcripts

A window on one transcript

Ensembl 2 transcripts

A window on one transcript