Reading the record for PHYH from HGNC, NCBI Gene and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 115 seconds for it, and its scripts then bring in the page, or a line saying what did not arrive.
The full name, the identifiers, the location and the notes from the sources arrive with the record. Nothing is filled in ahead of it.
Order door
The door to order for PHYH opens with the record, which decides which product it carries. The order page itself is open now.
Cytogenetic band 10p13NCBI: 10:13,277,799-13,300,064 on the minus strand, GRCh38.p14 (GCF_000001405.40), sequence NC_000010.11, annotation GCF_000001405.40-RS_2025_08 of 2025-08-01Ensembl: 10:13,276,854-13,302,412 on the minus strand, GRCh38.p14 (GCA_000001405.29), release 116Coordinates are one-based with both ends included, as each source reports them.
Reading NCBI Gene and UniProt.Still reading. A first read of a gene can take a while; this page waits up to 30 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
02 / Transcripts and isoforms
The RNA a design targets
Ready in a moment
Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
03 / Expression by tissue
Where PHYH is expressed
Ready in a moment
14 / Silencing this gene
From PHYH to a sequence that silences it
The AUMsilence™ platform designs the sequences against the human transcripts on this page. Six decisions are yours before it does. What is written under each is AUM's guidance; the timings and the concentrations are in the usage guide below.
01
Choose the region
A knockdown oligonucleotide can sit in the 5' untranslated region, the coding sequence or the 3' untranslated region, and all three are used. The coding sequence and the 3' untranslated region are the usual first choices for an RNase H design; the 5' end near the start codon suits a steric block. The map above shows where each region sits on the isoforms it draws.
02
Cover the isoforms you mean
An exon every isoform carries silences the whole gene; an exon only some isoforms carry silences those and spares the rest. Decide which you want before a sequence is chosen, and check the reference transcript (MANE Select in human; RefSeq Select and Ensembl canonical in mouse and rat) is the one your cells express.
03
Think across species early
The orthologs panel says whether mouse and rat carry the same gene. Whether one oligonucleotide can serve two species is a sequence question, settled at design by matching the candidate against each transcript, not by the protein identity shown there.
04
Check expression in your model
A transcript that is not expressed in your cells cannot show knockdown. Confirm the gene is expressed in the cell type and condition you will use, from your own data or a reference atlas, before the order. The expression panel above gives GTEx's median per tissue for a human gene; for mouse and rat it says that no atlas is on this page yet.
The median is the middle sample of a tissue, so a gene expressed in a few cells of a mixed tissue reads low here and may still be the one your cell type needs. Nothing on this panel is adjusted or averaged across tissues; each figure is the source's own, and a tissue GTEx did not sample is absent from the chart.
04 / Protein
The protein PHYH encodes
Ready in a moment
05 / Interactions
Proteins STRING associates with PHYH
Ready in a moment
06 / Pathways
Where PHYH acts, as Reactome curates it
Ready in a moment
07 / Disease associations
Diseases linked to PHYH
Ready in a moment
08 / Variants
Classified variants of PHYH
Ready in a moment
09 / Constraint
How much variation PHYH tolerates
Ready in a moment
10 / Orthologs
The same gene in mouse, rat and human
Ready in a moment
11 /MicroRNAs
MicroRNAs hosted within PHYH
Ready in a moment
12 / Long non-coding RNAs
Long non-coding RNAs at the PHYH locus
Ready in a moment
13 / Literature
Papers that mention PHYH
Ready in a moment
05
Run the controls
A scramble control of the same chemistry, a positive control against a gene known to knock down in your cells, untreated cells, and a mock condition where a transfection reagent is used. Read knockdown at the RNA level first, then at the protein; the usage guide gives the timing and the concentrations to start from.
06
Pick the product
AUMsilence sdASO needs no transfection reagent and works in the cells that resist one. AUMsilence toASO is the transfection-optimised version of the same design, and AUMsiRNA™ is the siRNA route. The selection guide compares them.
For research use only. Not for use in diagnostic or therapeutic procedures.
This gene is a member of the PhyH family and encodes a peroxisomal protein…
NCBI Gene summary
6 RefSeq and 43 Ensembl transcripts on GRCh38.p14; MANE Select NM_006214.4.
NCBI Datasets · Ensembl
54 GTEx tissues; the highest median in Muscle - Skeletal, 166 TPM.
GTEx
338 residues, reviewed O14832; 6 entries from the member databases this page shows along the chain; mean pLDDT 85.44; 1 PDB entry.
UniProt · InterPro · AlphaFold DB · PDBe
The 25 highest-scoring STRING partners at or above a combined score of 0.4, of up to 25 asked for; PHYHIP, PEX7, PEX5 lead.
STRING v12.0
3 curated, 0 inferred Reactome pathways for O14832 in human, v97.
Reactome
626 Open Targets disease associations; adult Refsum disease first, at 0.83.
Open Targets
ClinVar: 593 records for PHYH, 107 pathogenic or likely pathogenic.
ClinVar
pLI below 0.0001 and LOEUF 1.12 in gnomAD v4 (GRCh38), on ENST00000263038.9.
gnomAD
Mouse Phyh by 3 of 3 votes; rat Phyh by 3 of 3 votes. RGD is not answering, so the ortholog list could not be shown. Try again later. Reference fe55b5f5-ff1.
Alliance · NCBI · Ensembl Compara
No annotated microRNA lies within PHYH in Ensembl release 116, on GRCh38.p14.
Ensembl · miRBase
2 long non-coding RNA genes overlap PHYH in Ensembl release 116, all antisense, none with a symbol.
1,822 PubMed-indexed papers mention PHYH at Europe PMC, newest first. Europe PMC ignores letter case, so the count also covers another species' symbol spelled with the same letters.
Europe PMC
Gene summary
This gene is a member of the PhyH family and encodes a peroxisomal protein that is involved in the alpha-oxidation of 3-methyl branched fatty acids. Specifically, this protein converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA. Mutations in this gene have been associated with Refsum disease (RD) and deficient protein activity has been associated with Zellweger syndrome and rhizomelic chondrodysplasia punctata. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Provided by RefSeq, Jul 2008, through NCBI Gene. NCBI disclaimer
Catalyzes the 2-hydroxylation of not only racemic phytanoyl-CoA and the isomers of 3-methylhexadecanoyl-CoA, but also a variety of other mono-branched 3-methylacyl-CoA esters (with a chain length of at least seven carbon atoms) and straight-chain acyl-CoA esters (with a chain length longer than four carbon atoms) (PubMed:10744784, PubMed:12031666, PubMed:12923223, PubMed:9326939). Does not hydroxylate long and very long straight chain acyl-CoAs or 2-methyl- and 4-methyl-branched acyl-CoAs (PubMed:10744784, PubMed:12923223)
NCBI Gene summary · NCBI Gene annotation RS_2025_08 · read · NCBI Gene 5264Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
UniProtKB function · 2026_03 · read · UniProt O14832UniProt data are available under the Creative Commons Attribution 4.0 licence.
Placed on GRCh38.p14 (GCF_000001405.40). MANE Select marks the one transcript RefSeq and Ensembl agree is the reference for this gene.
RefSeq 6 transcripts
coding sequence, tall
untranslated region, thin
non-coding exon
intron, fixed width
Genomic strand: minus. Drawn 5' to 3', so exon 1 sits at the left here and at the highest coordinate on the chromosome.
NM_006214.4NM_006214.4MANE Select
NM_001323080.2NM_001323080.2
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
NCBI Datasets, RefSeq transcripts · NCBI Datasets 18.38.0; GCF_000001405.40-RS_2025_08 · read · NCBI Gene 5264Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
Ensembl 43 transcripts
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
Lengths are spliced lengths, as each source states them. Exon ranks follow the strand: on a minus-strand gene exon 1 has the highest genomic coordinate. Reference assembly for human: GRCh38.
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
