Reading the record for UBE4B from HGNC, NCBI Gene and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 115 seconds for it, and its scripts then bring in the page, or a line saying what did not arrive.
The full name, the identifiers, the location and the notes from the sources arrive with the record. Nothing is filled in ahead of it.
Order door
The door to order for UBE4B opens with the record, which decides which product it carries. The order page itself is open now.
Cytogenetic band 1p36.22NCBI: 1:10,032,958-10,181,239 on the plus strand, GRCh38.p14 (GCF_000001405.40), sequence NC_000001.11, annotation GCF_000001405.40-RS_2025_08 of 2025-08-01Ensembl: 1:10,032,832-10,181,244 on the plus strand, GRCh38.p14 (GCA_000001405.29), release 116Coordinates are one-based with both ends included, as each source reports them.
The modification of proteins with ubiquitin is an important cellular…
NCBI Gene summary
Ready in a moment
Reading NCBI Gene and UniProt.Still reading. A first read of a gene can take a while; this page waits up to 30 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
02 / Transcripts and isoforms
The RNA a design targets
8 RefSeq and 36 Ensembl transcripts on GRCh38.p14; MANE Select NM_001105562.3.
NCBI Datasets · Ensembl
Ready in a moment
Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
03 / Expression by tissue
Where UBE4B is expressed
Reading GTEx and the Human Protein Atlas.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
04 / Protein
The protein UBE4B encodes
Reading UniProt, InterPro, AlphaFold DB and PDBe.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
05 / Interactions
Proteins STRING associates with UBE4B
The 25 highest-scoring STRING partners at or above a combined score of 0.4, of up to 25 asked for; VCP, RAD23B, UFD1 lead.
STRING v12.0
Ready in a moment
06 / Pathways
Where UBE4B acts, as Reactome curates it
Reading UniProt and Reactome.Still reading. A first read of a gene can take a while; this page waits up to 60 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
07 / Disease associations
Diseases linked to UBE4B
855 Open Targets disease associations; neurodegenerative disease first, at 0.37.
Open Targets
Ready in a moment
08 / Variants
Classified variants of UBE4B
Reading ClinVar.Still reading. A first read of a gene can take a while; this page waits up to 60 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
09 / Constraint
How much variation UBE4B tolerates
Reading gnomAD and Open Targets.Still reading. A first read of a gene can take a while; this page waits up to 45 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
10 / Orthologs
The same gene in mouse, rat and human
Mouse Ube4b by 3 of 3 votes; rat Ube4b by 3 of 3 votes. RGD is not answering, so the ortholog list could not be shown. Try again later. Reference c90e8025-cba.
Alliance · NCBI · Ensembl Compara
Ready in a moment
11 /MicroRNAs
MicroRNAs hosted within UBE4B
No annotated microRNA lies within UBE4B in Ensembl release 116, on GRCh38.p14.
Ensembl · miRBase
Ready in a moment
12 / Long non-coding RNAs
Long non-coding RNAs at the UBE4B locus
No annotated long non-coding RNA overlaps UBE4B in Ensembl release 116, on GRCh38.p14.
Ensembl
Ready in a moment
13 / Literature
Papers that mention UBE4B
Reading Europe PMC.Still reading. A first read of a gene can take a while; this page waits up to 55 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
14 / Silencing this gene
From UBE4B to a sequence that silences it
The AUMsilence™ platform designs the sequences against the human transcripts on this page. Six decisions are yours before it does. What is written under each is AUM's guidance; the timings and the concentrations are in the usage guide below.
01
Choose the region
A knockdown oligonucleotide can sit in the 5' untranslated region, the coding sequence or the 3' untranslated region, and all three are used. The coding sequence and the 3' untranslated region are the usual first choices for an RNase H design; the 5' end near the start codon suits a steric block. The map above shows where each region sits on the isoforms it draws.
02
Cover the isoforms you mean
An exon every isoform carries silences the whole gene; an exon only some isoforms carry silences those and spares the rest. Decide which you want before a sequence is chosen, and check the reference transcript (MANE Select in human; RefSeq Select and Ensembl canonical in mouse and rat) is the one your cells express.
03
Think across species early
The orthologs panel says whether mouse and rat carry the same gene. Whether one oligonucleotide can serve two species is a sequence question, settled at design by matching the candidate against each transcript, not by the protein identity shown there.
04
Check expression in your model
A transcript that is not expressed in your cells cannot show knockdown. Confirm the gene is expressed in the cell type and condition you will use, from your own data or a reference atlas, before the order. The expression panel above gives GTEx's median per tissue for a human gene; for mouse and rat it says that no atlas is on this page yet.
05
Run the controls
A scramble control of the same chemistry, a positive control against a gene known to knock down in your cells, untreated cells, and a mock condition where a transfection reagent is used. Read knockdown at the RNA level first, then at the protein; the usage guide gives the timing and the concentrations to start from.
06
Pick the product
AUMsilence sdASO needs no transfection reagent and works in the cells that resist one. AUMsilence toASO is the transfection-optimised version of the same design, and AUMsiRNA™ is the siRNA route. The selection guide compares them.
For research use only. Not for use in diagnostic or therapeutic procedures.
Gene summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Provided by RefSeq, Jul 2008, through NCBI Gene. NCBI disclaimer
Acts as an E3 ubiquitin ligase and E4 ubiquitin chain elongation enzyme specifically involved in polyubiquitin chain assembly (PubMed:21317885). Plays an essential role in cardiac development and in the protection of neurons from degeneration evoked by ER stress (By similarity). Is recruited to early endosomes via interaction with the ESCRT-0 component HGS, where it ubiquitinates membrane protein cargo such as EGFR and APP to promote their sorting into multivesicular bodies and subsequent lysosomal degradation (PubMed:24344129, PubMed:32841720). Promotes MDM2-mediated polyubiquitination and subsequent degradation of the tumor suppressor TP53 (PubMed:21317885). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation (PubMed:17369820). Mediates the formation of 'Lys-11'- or 'Lys-48'-linked polyubiquitin chains on TRA2A and PYCR2 and induces their degradation by the proteasome (PubMed:35669517). Thereby regulates the alternative splicing function of TRA2A (PubMed:35669517). Also regulates the mTOR signaling pathway by polyubiquitinating KLHL22, an E3 ligase that degrades the GATOR1 complex protein DEPDC5 (By similarity). Under mitochondrial depolarization, mediates the ubiquitination of NIPSNAP1, thereby promoting its interaction with autophagy receptors NDP52 and p62/SQSTM1 and its subsequent degradation via the lysosome-dependent pathway (PubMed:41596759)
NCBI Gene summary · NCBI Gene annotation RS_2025_08 · read · NCBI Gene 10277Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
UniProtKB function · 2026_03 · read · UniProt O95155UniProt data are available under the Creative Commons Attribution 4.0 licence.
Placed on GRCh38.p14 (GCF_000001405.40). MANE Select marks the one transcript RefSeq and Ensembl agree is the reference for this gene.
RefSeq 8 transcripts
coding sequence, tall
untranslated region, thin
non-coding exon
intron, fixed width
Genomic strand: plus. Drawn 5' to 3', so exon 1 sits at the left here and at the lowest coordinate on the chromosome.
NM_001105562.3NM_001105562.3MANE Select
NM_001410744.1NM_001410744.1
NM_006048.5NM_006048.5
XM_047428018.1XM_047428018.1
XM_011540488.4XM_011540488.4
XM_011540489.4XM_011540489.4
XM_017000035.3XM_017000035.3
XM_011540490.3XM_011540490.3
scale
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other, except 1 block too short to see, widened to a fixed few pixels; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
Not listed, because the source places them on another assembly only: XM_054333796.1 (not placed on GRCh38.p14); XM_054333797.1 (not placed on GRCh38.p14); XM_054333798.1 (not placed on GRCh38.p14); XM_054333799.1 (not placed on GRCh38.p14); XM_054333800.1 (not placed on GRCh38.p14).
NCBI Datasets, RefSeq transcripts · NCBI Datasets 18.38.0; GCF_000001405.40-RS_2025_08 · read · NCBI Gene 10277Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
Ensembl 36 transcripts
coding sequence, tall
untranslated region, thin
non-coding exon
intron, fixed width
Genomic strand: plus. Drawn 5' to 3', so exon 1 sits at the left here and at the lowest coordinate on the chromosome.
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other, except 5 blocks too short to see, widened to a fixed few pixels; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
Lengths are spliced lengths, as each source states them. Exon ranks follow the strand: on a minus-strand gene exon 1 has the highest genomic coordinate. Reference assembly for human: GRCh38.
pLI above 0.9999 and LOEUF 0.251 in gnomAD v4 (GRCh38), on ENST00000343090.11.
gnomAD
54 GTEx tissues; the highest median in Brain - Cerebellar Hemisphere, 44.4 TPM.
GTEx
904 PubMed-indexed papers mention UBE4B at Europe PMC, newest first. Europe PMC ignores letter case, so the count also covers another species' symbol spelled with the same letters.
Europe PMC
Reactome v97 maps no pathway to O95155 in human.
Reactome
1,302 residues, reviewed O95155; 10 entries from the member databases this page shows along the chain; mean pLDDT 75; 4 PDB entries.
UniProt · InterPro · AlphaFold DB · PDBe
ClinVar: 230 records for UBE4B, 34 pathogenic or likely pathogenic.
