Gene Human Homo sapiens

USP31

ubiquitin specific peptidase 31

gene with protein product

Human USP31MouseRat

Reading the ortholog votes for mouse and rat.

Location: Cytogenetic band 16p12.2NCBI: 16:23,061,406-23,149,452 on the minus strand, GRCh38.p14 (GCF_000001405.40), sequence NC_000016.10, annotation GCF_000001405.40-RS_2025_08 of 2025-08-01Ensembl: 16:23,061,406-23,149,466 on the minus strand, GRCh38.p14 (GCA_000001405.29), release 116Coordinates are one-based with both ends included, as each source reports them.
Also known as: KIAA1203

Gene identity, from HGNC · HGNC 2026-10-02 · read 2026-10-03 ET · HGNC:20060

Silence this gene

The order page opens with USP31 and human filled in. The sequences are designed against the transcripts below; the price is on that page.

Order for USP31

sdASO, toASO or siRNA

Bring your own sequence Transcripts

For research use only. Not for use in diagnostic or therapeutic procedures.

Summary
Transcripts
Expression
Protein
Interactions
Pathways
Disease
Variants
Constraint
Orthologs
MicroRNAs
Long non-coding RNAs
Literature
Silencing this gene

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Ready in a moment

01 Summary and function

What USP31 does

Enables cysteine-type deubiquitinase activity.

NCBI Gene summary

Ready in a moment

02 Transcripts and isoforms

The RNA a design targets

Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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03 Expression by tissue

Where USP31 is expressed

Reading GTEx and the Human Protein Atlas.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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04 Protein

The protein USP31 encodes

Reading UniProt, InterPro, AlphaFold DB and PDBe.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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05 Interactions

Proteins STRING associates with USP31

Reading STRING.Still reading. A first read of a gene can take a while; this page waits up to 50 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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06 Pathways

Where USP31 acts, as Reactome curates it

Reading UniProt and Reactome.Still reading. A first read of a gene can take a while; this page waits up to 60 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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07 Disease associations

Diseases linked to USP31

589 Open Targets disease associations; temporomandibular joint disorder first, at 0.32.

Open Targets

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08 Variants

Classified variants of USP31

Reading ClinVar.Still reading. A first read of a gene can take a while; this page waits up to 60 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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09 Constraint

How much variation USP31 tolerates

pLI 0.9928 and LOEUF 0.474 in gnomAD v4 (GRCh38), on ENST00000219689.12.

gnomAD

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10 Orthologs

The same gene in mouse, rat and human

Reading the Alliance, NCBI, Ensembl Compara and RGD.Still reading. A first read of a gene can take a while; this page waits up to 145 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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11 MicroRNAs

MicroRNAs hosted within USP31

Reading Ensembl and miRBase.Still reading. A first read of a gene can take a while; this page waits up to 110 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

Ready in a moment

12 Long non-coding RNAs

Long non-coding RNAs at the USP31 locus

Reading Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 110 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

Ready in a moment

13 Literature

Papers that mention USP31

Reading Europe PMC.Still reading. A first read of a gene can take a while; this page waits up to 55 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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14 Silencing this gene

From USP31 to a sequence that silences it

The AUMsilence platform designs the sequences against the human transcripts on this page. Six decisions are yours before it does. What is written under each is AUM's guidance; the timings and the concentrations are in the usage guide below.

01
Choose the region
A knockdown oligonucleotide can sit in the 5' untranslated region, the coding sequence or the 3' untranslated region, and all three are used. The coding sequence and the 3' untranslated region are the usual first choices for an RNase H design; the 5' end near the start codon suits a steric block. The map above shows where each region sits on the isoforms it draws.
02
Cover the isoforms you mean
An exon every isoform carries silences the whole gene; an exon only some isoforms carry silences those and spares the rest. Decide which you want before a sequence is chosen, and check the reference transcript (MANE Select in human; RefSeq Select and Ensembl canonical in mouse and rat) is the one your cells express.
03
Think across species early
The orthologs panel says whether mouse and rat carry the same gene. Whether one oligonucleotide can serve two species is a sequence question, settled at design by matching the candidate against each transcript, not by the protein identity shown there.
04
Check expression in your model
A transcript that is not expressed in your cells cannot show knockdown. Confirm the gene is expressed in the cell type and condition you will use, from your own data or a reference atlas, before the order. The expression panel above gives GTEx's median per tissue for a human gene; for mouse and rat it says that no atlas is on this page yet.
05
Run the controls
A scramble control of the same chemistry, a positive control against a gene known to knock down in your cells, untreated cells, and a mock condition where a transfection reagent is used. Read knockdown at the RNA level first, then at the protein; the usage guide gives the timing and the concentrations to start from.
06
Pick the product
AUMsilence sdASO needs no transfection reagent and works in the cells that resist one. AUMsilence toASO is the transfection-optimised version of the same design, and AUMsiRNA is the siRNA route. The selection guide compares them.

Order for USP31 Bring your own sequence The usage guide Selection guide

For research use only. Not for use in diagnostic or therapeutic procedures.

Gene Human Homo sapiens

USP31

Reading the record for USP31 from HGNC, NCBI Gene and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 115 seconds for it, and its scripts then bring in the page, or a line saying what did not arrive.

The full name, the identifiers, the location and the notes from the sources arrive with the record. Nothing is filled in ahead of it.

Order door

The door to order for USP31 opens with the record, which decides which product it carries. The order page itself is open now.

Bring your own sequence

For research use only. Not for use in diagnostic or therapeutic procedures.

This page is still reading the record. Its scripts replace this shell with the record when it arrives; with scripts off, it stays at this shell however long you wait.

01 Summary and function

What USP31 does

Waiting for the record before reading NCBI Gene and UniProt.

02 Transcripts and isoforms

The RNA a design targets

Waiting for the record before reading NCBI Datasets and Ensembl.

03 Expression by tissue

Where USP31 is expressed

Waiting for the record before reading GTEx and the Human Protein Atlas.

04 Protein

The protein USP31 encodes

Waiting for the record before reading UniProt, InterPro, AlphaFold DB and PDBe.

05 Interactions

Proteins STRING associates with USP31

Waiting for the record before reading STRING.

06 Pathways

Where USP31 acts, as Reactome curates it

Waiting for the record before reading UniProt and Reactome.

07 Disease associations

Diseases linked to USP31

Waiting for the record before reading Open Targets and ClinGen.

08 Variants

Classified variants of USP31

Waiting for the record before reading ClinVar.

09 Constraint

How much variation USP31 tolerates

Waiting for the record before reading gnomAD and Open Targets.

10 Orthologs

The same gene in mouse, rat and human

Waiting for the record before reading the Alliance, NCBI, Ensembl Compara and RGD.

11 MicroRNAs

MicroRNAs hosted within USP31

Waiting for the record before reading Ensembl and miRBase.

12 Long non-coding RNAs

Long non-coding RNAs at the USP31 locus

Waiting for the record before reading Ensembl.

13 Literature

Papers that mention USP31

Waiting for the record before reading Europe PMC.

Accession	Name	Biotype	Labels	Exons	Spliced length	Protein	Sequence
NM_020718.4matches ENST00000219689.12	transcript variant 1	protein coding	MANE Select	16	10,881 nt	1,352 aaNP_065769.3	Ready in a moment
XM_017023497.3	transcript variant X1	protein coding model	none	16	17,108 nt	1,089 aaXP_016878986.1	Ready in a moment
NM_001387221.1matches ENST00000953487.2	transcript variant 2	protein coding	none	15	10,761 nt	1,312 aaNP_001374150.1	Ready in a moment
XM_017023498.3	transcript variant X2	Ready in a moment

Accession	Name	Biotype	Labels	Exons	Spliced length	Protein	Sequence
NM_020718.4matches ENST00000219689.12	transcript variant 1	protein coding	MANE Select	16	10,881 nt	1,352 aaNP_065769.3	Ready in a moment
XM_017023497.3	transcript variant X1	protein coding model	none	16	17,108 nt	1,089 aaXP_016878986.1	Ready in a moment
NM_001387221.1matches ENST00000953487.2	transcript variant 2	protein coding	none	15	10,761 nt	1,312 aaNP_001374150.1	Ready in a moment
XM_017023498.3	transcript variant X2	Ready in a moment

Accession	Name	Biotype	Labels	Exons	Spliced length	Protein	Sequence
ENST00000219689.12matches NM_020718.4	USP31-201	protein coding	MANE SelectEnsembl canonical	16	10,881 nt	1,352 aaENSP00000219689.7	Ready in a moment
ENST00001136791.1	USP31-210	protein coding	none	16	10,895 nt	1,352 aaENSP00000801662.1	Ready in a moment
ENST00000953487.2	USP31-204	protein coding	none	15	10,775 nt	1,312 aaENSP00000623546.1	Ready in a moment
ENST00000567975.2	USP31-203	protein coding	none	2	8,715 nt	645 aaENSP00000461621.1	Ready in a moment
ENST00001014654.1	USP31-205	nonsense mediated decay	none	16	6,041 nt	711 aaENSP00000684471.1	Ready in a moment
ENST00001014655.1	USP31-206	nonsense mediated decay	none	17	4,385 nt	280 aaENSP00000684472.1	Ready in a moment
ENST00001014656.1	USP31-207	protein coding	none	12	3,265 nt	767 aaENSP00000684473.1	Ready in a moment
ENST00001014657.1	USP31-208	protein coding	none	12	3,217 nt	753 aaENSP00000684474.1	Ready in a moment
ENST00001111960.1	USP31-209	protein coding	none	12	2,558 nt	685 aaENSP00000781765.1	Ready in a moment
ENST00000563525.2	USP31-202	protein coding	none	5	1,560 nt	266 aaENSP00000481339.1	Ready in a moment

USP31

What USP31 does

The RNA a design targets

Where USP31 is expressed

The protein USP31 encodes

Proteins STRING associates with USP31

Where USP31 acts, as Reactome curates it

Diseases linked to USP31

Classified variants of USP31

How much variation USP31 tolerates

The same gene in mouse, rat and human

MicroRNAs hosted within USP31

Long non-coding RNAs at the USP31 locus

Papers that mention USP31

From USP31 to a sequence that silences it

Choose the region

Cover the isoforms you mean

Think across species early

Check expression in your model

Run the controls

Pick the product

USP31

What USP31 does

The RNA a design targets

Where USP31 is expressed

The protein USP31 encodes

Proteins STRING associates with USP31

Where USP31 acts, as Reactome curates it

Diseases linked to USP31

Classified variants of USP31

How much variation USP31 tolerates

The same gene in mouse, rat and human

MicroRNAs hosted within USP31

Long non-coding RNAs at the USP31 locus

Papers that mention USP31

USP31

What USP31 does

Gene summary

Protein function

The RNA a design targets

RefSeq 7 transcripts

A window on one transcript

Ensembl 10 transcripts

A window on one transcript

Where USP31 is expressed

The protein USP31 encodes

Proteins STRING associates with USP31

Where USP31 acts, as Reactome curates it

Diseases linked to USP31

Classified variants of USP31

How much variation USP31 tolerates

The same gene in mouse, rat and human

MicroRNAs hosted within USP31

Long non-coding RNAs at the USP31 locus

Papers that mention USP31

From USP31 to a sequence that silences it

Choose the region

Cover the isoforms you mean

Think across species early

Check expression in your model

Run the controls

Pick the product

Gene summary

Protein function

RefSeq 7 transcripts

A window on one transcript

Ensembl 10 transcripts

A window on one transcript