Reading the record for Aak1 from NCBI Gene and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 115 seconds for it, and its scripts then bring in the page, or a line saying what did not arrive.
The full name, the identifiers, the location and the notes from the sources arrive with the record. Nothing is filled in ahead of it.
Order door
The door to order for Aak1 opens with the record, which decides which product it carries. The order page itself is open now.
HGNC and MANE Select: not available for mouse. HGNC names human genes; MGI is the authority here, and MANE Select is defined for human transcripts only.
Location
Cytogenetic band 6 D1NCBI: 6:86,826,252-86,980,209 on the plus strand, GRCm39 (GCF_000001635.27), sequence NC_000072.7, annotation GCF_000001635.27-RS_2024_02 of 2024-02-01Ensembl: no location was read.Coordinates are one-based with both ends included, as each source reports them.
Reading NCBI Gene and UniProt.Still reading. A first read of a gene can take a while; this page waits up to 30 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
02 / Transcripts and isoforms
The RNA a design targets
44 RefSeq transcripts on GRCm39; RefSeq Select NM_001378654.1. Ensembl is not answering, so the Ensembl transcripts could not be shown. Try again later. Reference cf000e4e-ea7.
NCBI Datasets
Ready in a moment
Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
03 / Expression by tissue
Where Aak1 is expressed
Expression by tissue: not available for mouse. GTEx holds human tissues only, and the Human Protein Atlas is keyed on human genes.
Ready in a moment
Reading GTEx and the Human Protein Atlas.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
04 / Protein
The protein Aak1 encodes
Aak1 is linked to 2 UniProt accessions and none was chosen; the candidates are listed
UniProt
Ready in a moment
05 / Interactions
Proteins STRING associates with Aak1
Reading STRING.Still reading. A first read of a gene can take a while; this page waits up to 50 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
06 / Pathways
Where Aak1 acts, as Reactome curates it
Aak1 is linked to 2 UniProt accessions and none was chosen; the candidates are listed
UniProt
Ready in a moment
07 / Disease associations
Diseases linked to Aak1
Disease associations: not available for mouse. Open Targets and ClinGen curate human genes only. The human ortholog's page carries the associations.
Ready in a moment
Not available
Disease associations: not available for mouse. Open Targets and ClinGen curate human genes only. The human ortholog's page carries the associations.
08 / Variants
Classified variants of Aak1
Clinical variants: not available for mouse. ClinVar holds human variants only.
Ready in a moment
Not available
Clinical variants: not available for mouse. ClinVar holds human variants only.
09 / Constraint
How much variation Aak1 tolerates
Constraint: not available for mouse. gnomAD and Open Targets carry constraint for human genes only.
Ready in a moment
Not available
Constraint: not available for mouse. gnomAD and Open Targets carry constraint for human genes only.
10 / Orthologs
The same gene in mouse, rat and human
Reading the Alliance, NCBI, Ensembl Compara and RGD.Still reading. A first read of a gene can take a while; this page waits up to 145 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
11 /MicroRNAs
MicroRNAs hosted within Aak1
Ensembl is not answering, so the non-coding annotation could not be shown. Try again later. Reference ec7a56cd-348.
Ready in a moment
12 / Long non-coding RNAs
Long non-coding RNAs at the Aak1 locus
Ensembl is not answering, so the non-coding annotation could not be shown. Try again later. Reference ec7a56cd-348.
Ready in a moment
13 / Literature
Papers that mention Aak1
1,899 PubMed-indexed papers mention Aak1 at Europe PMC, newest first. Europe PMC ignores letter case, so the count also covers the human symbol where it differs from this one only in case.
Europe PMC
Ready in a moment
14 / Silencing this gene
From Aak1 to a sequence that silences it
The AUMsilence™ platform designs the sequences against the mouse transcripts on this page. Six decisions are yours before it does. What is written under each is AUM's guidance; the timings and the concentrations are in the usage guide below.
01
Choose the region
A knockdown oligonucleotide can sit in the 5' untranslated region, the coding sequence or the 3' untranslated region, and all three are used. The coding sequence and the 3' untranslated region are the usual first choices for an RNase H design; the 5' end near the start codon suits a steric block. The map above shows where each region sits on the isoforms it draws.
02
Cover the isoforms you mean
An exon every isoform carries silences the whole gene; an exon only some isoforms carry silences those and spares the rest. Decide which you want before a sequence is chosen, and check the reference transcript (MANE Select in human; RefSeq Select and Ensembl canonical in mouse and rat) is the one your cells express.
03
Think across species early
The orthologs panel says whether mouse and rat carry the same gene. Whether one oligonucleotide can serve two species is a sequence question, settled at design by matching the candidate against each transcript, not by the protein identity shown there.
04
Check expression in your model
A transcript that is not expressed in your cells cannot show knockdown. Confirm the gene is expressed in the cell type and condition you will use, from your own data or a reference atlas, before the order. The expression panel above gives GTEx's median per tissue for a human gene; for mouse and rat it says that no atlas is on this page yet.
05
Run the controls
A scramble control of the same chemistry, a positive control against a gene known to knock down in your cells, untreated cells, and a mock condition where a transfection reagent is used. Read knockdown at the RNA level first, then at the protein; the usage guide gives the timing and the concentrations to start from.
06
Pick the product
AUMsilence sdASO needs no transfection reagent and works in the cells that resist one. AUMsilence toASO is the transfection-optimised version of the same design, and AUMsiRNA™ is the siRNA route. The selection guide compares them.
For research use only. Not for use in diagnostic or therapeutic procedures.
Gene summary
Predicted to enable AP-2 adaptor complex binding activity; Notch binding activity; and protein serine/threonine kinase activity. Involved in presynaptic endocytosis. Predicted to be located in several cellular components, including clathrin-coated pit; clathrin-coated vesicle; and terminal bouton. Predicted to be part of clathrin complex. Predicted to be active in calyx of Held. Is expressed in cranial ganglion; dorsal root ganglion; retina nuclear layer; and telencephalon. Orthologous to human AAK1 (AP2 associated kinase 1).
Provided by Alliance of Genome Resources, Jul 2025, through NCBI Gene. NCBI disclaimer
Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Preferentially, may phosphorylate substrates on threonine residues. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity
this gene is linked to 2 reviewed UniProt entries, each a protein of its own; none is chosen
NCBI Gene summary · NCBI Gene annotation RS_2024_02 · read · NCBI Gene 269774Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
UniProtKB function · 2026_03 · read · UniProt Q3UHJ0UniProt data are available under the Creative Commons Attribution 4.0 licence.
UniProtKB function · 2026_03 · read · UniProt Q6PIU9UniProt data are available under the Creative Commons Attribution 4.0 licence.
Placed on GRCm39 (GCF_000001635.27). MANE Select: not available for mouse. The reference here is the RefSeq Select transcript and the Ensembl canonical transcript, which need not be the same model.
RefSeq 44 transcripts
coding sequence, tall
untranslated region, thin
non-coding exon
intron, fixed width
Genomic strand: plus. Drawn 5' to 3', so exon 1 sits at the left here and at the lowest coordinate on the chromosome.
NM_001378654.1NM_001378654.1RefSeq Select
XM_036166135.1XM_036166135.1
XM_036166139.1XM_036166139.1
XM_036166140.1XM_036166140.1
XM_036166141.1XM_036166141.1
XM_036166136.1XM_036166136.1
XM_036166138.1XM_036166138.1
XM_036166137.1XM_036166137.1
XM_036166143.1XM_036166143.1
XM_036166144.1XM_036166144.1
XM_036166147.1XM_036166147.1
XM_036166145.1XM_036166145.1
XM_036166148.1XM_036166148.1
XM_036166142.1XM_036166142.1
XM_036166162.1XM_036166162.1
XM_036166161.1XM_036166161.1
XM_036166151.1XM_036166151.1
XM_036166146.1XM_036166146.1
XM_036166153.1XM_036166153.1
XM_036166155.1XM_036166155.1
XM_036166149.1XM_036166149.1
XM_036166152.1XM_036166152.1
XM_036166158.1XM_036166158.1
XM_036166150.1XM_036166150.1
XM_036166157.1XM_036166157.1
XM_036166164.1XM_036166164.1
XM_036166154.1XM_036166154.1
XM_036166156.1XM_036166156.1
NM_001378652.1NM_001378652.1
XM_036166159.1XM_036166159.1
XM_036166160.1XM_036166160.1
NM_001378655.1NM_001378655.1
XM_036166163.1XM_036166163.1
NM_001040106.3NM_001040106.3
XM_006506192.3XM_006506192.3
XM_030255413.2XM_030255413.2
XM_030255416.2XM_030255416.2
NM_177762.7NM_177762.7
XM_017321597.2XM_017321597.2
XM_036166165.1XM_036166165.1
XM_030255415.2XM_030255415.2
XM_036166166.1XM_036166166.1
XM_036166167.1XM_036166167.1
XM_030255418.2XM_030255418.2
scale
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other, except 5 blocks too short to see, widened to a fixed few pixels; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
NCBI Datasets, RefSeq transcripts · NCBI Datasets 18.37.0; GCF_000001635.27-RS_2024_02 · read · NCBI Gene 269774Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
Ensembl 0 transcripts
Ensembl is not answering, so the Ensembl transcripts could not be shown. Try again later.
Reference cf000e4e-ea7
Lengths are spliced lengths, as each source states them. Exon ranks follow the strand: on a minus-strand gene exon 1 has the highest genomic coordinate. Reference assembly for mouse: GRCm39.
Expression by tissue: not available for mouse. GTEx holds human tissues only, and the Human Protein Atlas is keyed on human genes.
Mouse tissue expression, from Bgee and Expression Atlas, joins this page in a later phase. Nothing from the human ortholog is shown in its place; its own page is a step away through the species switch above.
Human AAK1 by 2 of 2 votes; rat Aak1 by 2 of 2 votes. RGD is not answering, so the ortholog list could not be shown. Try again later. Reference 23cd00b0-3c9.
Alliance · NCBI
The 25 highest-scoring STRING partners at or above a combined score of 0.4, of up to 25 asked for; Ap2m1, Eps15, Ap2b1 lead.
