Reading the record for Actn4 from NCBI Gene and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 115 seconds for it, and its scripts then bring in the page, or a line saying what did not arrive.
The full name, the identifiers, the location and the notes from the sources arrive with the record. Nothing is filled in ahead of it.
Order door
The door to order for Actn4 opens with the record, which decides which product it carries. The order page itself is open now.
HGNC and MANE Select: not available for mouse. HGNC names human genes; MGI is the authority here, and MANE Select is defined for human transcripts only.
Location
Cytogenetic band 7 B1NCBI: 7:28,592,673-28,661,799 on the minus strand, GRCm39 (GCF_000001635.27), sequence NC_000073.7, annotation GCF_000001635.27-RS_2024_02 of 2024-02-01Ensembl: 7:28,592,672-28,661,839 on the minus strand, GRCm39 (GCA_000001635.9), release 116Coordinates are one-based with both ends included, as each source reports them.
Gene identity, from NCBI Datasets · NCBI Datasets 18.38.0 · read · MGI:1890773Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
Silence this gene
The order page opens with Actn4 and mouse filled in. The sequences are designed against the transcripts below; the price is on that page.
Reading NCBI Gene and UniProt.Still reading. A first read of a gene can take a while; this page waits up to 30 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
02 / Transcripts and isoforms
The RNA a design targets
Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
03 / Expression by tissue
Where Actn4 is expressed
Expression by tissue: not available for mouse. GTEx holds human tissues only, and the Human Protein Atlas is keyed on human genes.
Ready in a moment
Reading GTEx and the Human Protein Atlas.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
04 / Protein
The protein Actn4 encodes
Reading UniProt, InterPro, AlphaFold DB and PDBe.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
05 / Interactions
Proteins STRING associates with Actn4
Reading STRING.Still reading. A first read of a gene can take a while; this page waits up to 50 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
06 / Pathways
Where Actn4 acts, as Reactome curates it
Reading UniProt and Reactome.Still reading. A first read of a gene can take a while; this page waits up to 60 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
07 / Disease associations
Diseases linked to Actn4
Disease associations: not available for mouse. Open Targets and ClinGen curate human genes only. The human ortholog's page carries the associations.
Ready in a moment
Not available
Disease associations: not available for mouse. Open Targets and ClinGen curate human genes only. The human ortholog's page carries the associations.
08 / Variants
Classified variants of Actn4
Clinical variants: not available for mouse. ClinVar holds human variants only.
Ready in a moment
Not available
Clinical variants: not available for mouse. ClinVar holds human variants only.
09 / Constraint
How much variation Actn4 tolerates
Constraint: not available for mouse. gnomAD and Open Targets carry constraint for human genes only.
Ready in a moment
Not available
Constraint: not available for mouse. gnomAD and Open Targets carry constraint for human genes only.
10 / Orthologs
The same gene in mouse, rat and human
Reading the Alliance, NCBI, Ensembl Compara and RGD.Still reading. A first read of a gene can take a while; this page waits up to 145 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
11 /MicroRNAs
MicroRNAs hosted within Actn4
Reading Ensembl and miRBase.Still reading. A first read of a gene can take a while; this page waits up to 110 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
12 / Long non-coding RNAs
Long non-coding RNAs at the Actn4 locus
Reading Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 110 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
13 / Literature
Papers that mention Actn4
Reading Europe PMC.Still reading. A first read of a gene can take a while; this page waits up to 55 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
14 / Silencing this gene
From Actn4 to a sequence that silences it
The AUMsilence™ platform designs the sequences against the mouse transcripts on this page. Six decisions are yours before it does. What is written under each is AUM's guidance; the timings and the concentrations are in the usage guide below.
01
Choose the region
A knockdown oligonucleotide can sit in the 5' untranslated region, the coding sequence or the 3' untranslated region, and all three are used. The coding sequence and the 3' untranslated region are the usual first choices for an RNase H design; the 5' end near the start codon suits a steric block. The map above shows where each region sits on the isoforms it draws.
02
Cover the isoforms you mean
An exon every isoform carries silences the whole gene; an exon only some isoforms carry silences those and spares the rest. Decide which you want before a sequence is chosen, and check the reference transcript (MANE Select in human; RefSeq Select and Ensembl canonical in mouse and rat) is the one your cells express.
03
Think across species early
The orthologs panel says whether mouse and rat carry the same gene. Whether one oligonucleotide can serve two species is a sequence question, settled at design by matching the candidate against each transcript, not by the protein identity shown there.
04
Check expression in your model
A transcript that is not expressed in your cells cannot show knockdown. Confirm the gene is expressed in the cell type and condition you will use, from your own data or a reference atlas, before the order. The expression panel above gives GTEx's median per tissue for a human gene; for mouse and rat it says that no atlas is on this page yet.
05
Run the controls
A scramble control of the same chemistry, a positive control against a gene known to knock down in your cells, untreated cells, and a mock condition where a transfection reagent is used. Read knockdown at the RNA level first, then at the protein; the usage guide gives the timing and the concentrations to start from.
06
Pick the product
AUMsilence sdASO needs no transfection reagent and works in the cells that resist one. AUMsilence toASO is the transfection-optimised version of the same design, and AUMsiRNA™ is the siRNA route. The selection guide compares them.
For research use only. Not for use in diagnostic or therapeutic procedures.
Gene summary
Enables actin filament binding activity. A structural constituent of cytoskeleton. Involved in several processes, including bicellular tight junction assembly; podocyte cell migration; and protein localization to bicellular tight junction. Acts upstream of or within gene expression. Located in several cellular components, including Z disc; focal adhesion; and pseudopodium. Colocalizes with stress fiber. Is expressed in 4-cell stage conceptus; blastocyst-stage conceptus; heart; and morula-stage conceptus. Used to study focal segmental glomerulosclerosis 1. Human ortholog(s) of this gene implicated in focal segmental glomerulosclerosis and focal segmental glomerulosclerosis 1. Orthologous to human ACTN4 (actinin alpha 4).
Provided by Alliance of Genome Resources, Jun 2026, through NCBI Gene. NCBI disclaimer
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (By similarity). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (PubMed:18332111). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (By similarity). Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation (By similarity)
NCBI Gene summary · NCBI Gene annotation RS_2024_02 · read · NCBI Gene 60595Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
UniProtKB function · 2026_03 · read · UniProt P57780UniProt data are available under the Creative Commons Attribution 4.0 licence.
Expression by tissue: not available for mouse. GTEx holds human tissues only, and the Human Protein Atlas is keyed on human genes.
Mouse tissue expression, from Bgee and Expression Atlas, joins this page in a later phase. Nothing from the human ortholog is shown in its place; its own page is a step away through the species switch above.
No annotated microRNA lies within Actn4 in Ensembl release 116, on GRCm39.
Ensembl · miRBase
2 long non-coding RNA genes overlap Actn4 in Ensembl release 116, 1 antisense: Gm44699, Gm38978.
5,370 PubMed-indexed papers mention Actn4 at Europe PMC, newest first. Europe PMC ignores letter case, so the count also covers the human symbol where it differs from this one only in case.
Europe PMC
0 curated, 1 inferred Reactome pathways for P57780 in mouse, v97.
Reactome
15 RefSeq and 40 Ensembl transcripts on GRCm39; RefSeq Select NM_021895.4; Ensembl canonical ENSMUST00000217157.3.
NCBI Datasets · Ensembl
Placed on GRCm39 (GCF_000001635.27). MANE Select: not available for mouse. The reference here is the RefSeq Select transcript and the Ensembl canonical transcript, which need not be the same model.
RefSeq 15 transcripts
coding sequence, tall
untranslated region, thin
non-coding exon
intron, fixed width
Genomic strand: minus. Drawn 5' to 3', so exon 1 sits at the left here and at the highest coordinate on the chromosome.
NM_021895.4NM_021895.4RefSeq Select
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
NCBI Datasets, RefSeq transcripts · NCBI Datasets 18.38.0; GCF_000001635.27-RS_2024_02 · read · NCBI Gene 60595Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
Ensembl 40 transcripts
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
Lengths are spliced lengths, as each source states them. Exon ranks follow the strand: on a minus-strand gene exon 1 has the highest genomic coordinate. Reference assembly for mouse: GRCm39.
XM_036153312.1XM_036153312.1
XM_036153311.1XM_036153311.1
XM_006540257.4XM_006540257.4
NM_001360550.2NM_001360550.2
NM_001360548.2NM_001360548.2
NM_001420183.1NM_001420183.1
NM_001420184.1NM_001420184.1
NM_001360549.2NM_001360549.2
NM_001420185.1NM_001420185.1
NM_001420186.1NM_001420186.1
NM_001420187.1NM_001420187.1
NM_001420188.1NM_001420188.1
NM_001420189.1NM_001420189.1
XR_004934159.1XR_004934159.1
scale
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
The 25 highest-scoring STRING partners at or above a combined score of 0.4, of up to 25 asked for; Vcl, Actn1, Magi1 lead.
STRING v12.0
Human ACTN4 by 3 of 3 votes; rat Actn4 by 3 of 3 votes. RGD is not answering, so the ortholog list could not be shown. Try again later. Reference d915b6e2-ef0.
Alliance · NCBI · Ensembl Compara
912 residues, reviewed P57780; 23 entries from the member databases this page shows along the chain; mean pLDDT 84.12; no experimental structure at PDBe.
