Gene Mouse Mus musculus

Dio1

deiodinase, iodothyronine, type I

protein coding

HumanMouse Dio1Rat

Reading the ortholog votes for human and rat.

HGNC and MANE Select: not available for mouse. HGNC names human genes; MGI is the authority here, and MANE Select is defined for human transcripts only.

Location: Cytogenetic band 4 C7NCBI: 4:107,148,662-107,164,365 on the minus strand, GRCm39 (GCF_000001635.27), sequence NC_000070.7, annotation GCF_000001635.27-RS_2024_02 of 2024-02-01Ensembl: 4:107,148,662-107,164,366 on the minus strand, GRCm39 (GCA_000001635.9), release 116Coordinates are one-based with both ends included, as each source reports them.
Also known as: D1, 5DI, ITDI1, TXDI1

Gene identity, from NCBI Datasets · NCBI Datasets 18.38.0 · read 2026-10-02 ET · MGI:94896Data from NCBI, provided as is; NCBI's policies and disclaimers apply.

Silence this gene

The order page opens with Dio1 and mouse filled in. The sequences are designed against the transcripts below; the price is on that page.

Order for Dio1

sdASO, toASO or siRNA, chosen on the next page

Bring your own sequence Transcripts

For research use only. Not for use in diagnostic or therapeutic procedures.

Summary
Transcripts
Expression
Protein
Interactions
Pathways
Disease
Variants
Constraint
Orthologs
MicroRNAs
Long non-coding RNAs
Literature
Silencing this gene

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Ready in a moment

01 Summary and function

What Dio1 does

The protein encoded by this gene belongs to the iodothyronine deiodinase…

NCBI Gene summary

Ready in a moment

02 Transcripts and isoforms

The RNA a design targets

Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

Ready in a moment

03 Expression by tissue

Where Dio1 is expressed

Expression by tissue: not available for mouse. GTEx holds human tissues only, and the Human Protein Atlas is keyed on human genes.

Ready in a moment

04 Protein

The protein Dio1 encodes

Reading UniProt, InterPro, AlphaFold DB and PDBe.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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05 Interactions

Proteins STRING associates with Dio1

The 20 highest-scoring STRING partners at or above a combined score of 0.4, of up to 25 asked for; Dio2, Thra, Selenot lead.

STRING v12.0

Ready in a moment

06 Pathways

Where Dio1 acts, as Reactome curates it

Reading UniProt and Reactome.Still reading. A first read of a gene can take a while; this page waits up to 60 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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07 Disease associations

Diseases linked to Dio1

Disease associations: not available for mouse. Open Targets and ClinGen curate human genes only. The human ortholog's page carries the associations.

Ready in a moment

08 Variants

Classified variants of Dio1

Clinical variants: not available for mouse. ClinVar holds human variants only.

Ready in a moment

09 Constraint

How much variation Dio1 tolerates

Constraint: not available for mouse. gnomAD and Open Targets carry constraint for human genes only.

Ready in a moment

10 Orthologs

The same gene in mouse, rat and human

Reading the Alliance, NCBI, Ensembl Compara and RGD.Still reading. A first read of a gene can take a while; this page waits up to 145 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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11 MicroRNAs

MicroRNAs hosted within Dio1

Reading Ensembl and miRBase.Still reading. A first read of a gene can take a while; this page waits up to 110 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

Ready in a moment

12 Long non-coding RNAs

Long non-coding RNAs at the Dio1 locus

Reading Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 110 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

Ready in a moment

13 Literature

Papers that mention Dio1

Reading Europe PMC.Still reading. A first read of a gene can take a while; this page waits up to 55 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

Ready in a moment

14 Silencing this gene

From Dio1 to a sequence that silences it

The AUMsilence platform designs the sequences against the mouse transcripts on this page. Six decisions are yours before it does. What is written under each is AUM's guidance; the timings and the concentrations are in the usage guide below.

01
Choose the region
A knockdown oligonucleotide can sit in the 5' untranslated region, the coding sequence or the 3' untranslated region, and all three are used. The coding sequence and the 3' untranslated region are the usual first choices for an RNase H design; the 5' end near the start codon suits a steric block. The map above shows where each region sits on the isoforms it draws.
02
Cover the isoforms you mean
An exon every isoform carries silences the whole gene; an exon only some isoforms carry silences those and spares the rest. Decide which you want before a sequence is chosen, and check the reference transcript (MANE Select in human; RefSeq Select and Ensembl canonical in mouse and rat) is the one your cells express.
03
Think across species early
The orthologs panel says whether mouse and rat carry the same gene. Whether one oligonucleotide can serve two species is a sequence question, settled at design by matching the candidate against each transcript, not by the protein identity shown there.
04
Check expression in your model
A transcript that is not expressed in your cells cannot show knockdown. Confirm the gene is expressed in the cell type and condition you will use, from your own data or a reference atlas, before the order. The expression panel above gives GTEx's median per tissue for a human gene; for mouse and rat it says that no atlas is on this page yet.
05
Run the controls
A scramble control of the same chemistry, a positive control against a gene known to knock down in your cells, untreated cells, and a mock condition where a transfection reagent is used. Read knockdown at the RNA level first, then at the protein; the usage guide gives the timing and the concentrations to start from.
06
Pick the product
AUMsilence sdASO needs no transfection reagent and works in the cells that resist one. AUMsilence toASO is the transfection-optimised version of the same design, and AUMsiRNA is the siRNA route. The selection guide compares them.

Order for Dio1 Bring your own sequence The usage guide Selection guide

For research use only. Not for use in diagnostic or therapeutic procedures.

Gene Mouse Mus musculus

Dio1

Reading the record for Dio1 from NCBI Gene and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 115 seconds for it, and its scripts then bring in the page, or a line saying what did not arrive.

The full name, the identifiers, the location and the notes from the sources arrive with the record. Nothing is filled in ahead of it.

Order door

The door to order for Dio1 opens with the record, which decides which product it carries. The order page itself is open now.

Bring your own sequence

For research use only. Not for use in diagnostic or therapeutic procedures.

This page is still reading the record. Its scripts replace this shell with the record when it arrives; with scripts off, it stays at this shell however long you wait.

01 Summary and function

What Dio1 does

Waiting for the record before reading NCBI Gene and UniProt.

02 Transcripts and isoforms

The RNA a design targets

Waiting for the record before reading NCBI Datasets and Ensembl.

03 Expression by tissue

Where Dio1 is expressed

Waiting for the record before reading GTEx and the Human Protein Atlas.

04 Protein

The protein Dio1 encodes

Waiting for the record before reading UniProt, InterPro, AlphaFold DB and PDBe.

05 Interactions

Proteins STRING associates with Dio1

Waiting for the record before reading STRING.

06 Pathways

Where Dio1 acts, as Reactome curates it

Waiting for the record before reading UniProt and Reactome.

07 Disease associations

Diseases linked to Dio1

Waiting for the record before reading Open Targets and ClinGen.

08 Variants

Classified variants of Dio1

Waiting for the record before reading ClinVar.

09 Constraint

How much variation Dio1 tolerates

Waiting for the record before reading gnomAD and Open Targets.

10 Orthologs

The same gene in mouse, rat and human

Waiting for the record before reading the Alliance, NCBI, Ensembl Compara and RGD.

11 MicroRNAs

MicroRNAs hosted within Dio1

Waiting for the record before reading Ensembl and miRBase.

12 Long non-coding RNAs

Long non-coding RNAs at the Dio1 locus

Waiting for the record before reading Ensembl.

13 Literature

Papers that mention Dio1

Waiting for the record before reading Europe PMC.

Accession	Name	Biotype	Labels	Exons	Spliced length	Protein	Sequence
ENSMUST00000082426.11	Dio1-201	protein coding	Ensembl canonical	4	1,690 nt	257 aaENSMUSP00000081007.5	Ready in a moment
ENSMUST00000150974.2	Dio1-208	nonsense mediated decay	none	3	761 nt	125 aaENSMUSP00000117751.2	Ready in a moment
ENSMUST00000134366.8	Dio1-205	nonsense mediated decay	none	4	671 nt	82 aaENSMUSP00000119199.2	Ready in a moment
ENSMUST00000129138.2	Dio1-204	protein coding	none	3	632 nt	208 aaENSMUSP00000118335.2	Ready in a moment
ENSMUST00000126291.2	Dio1-203	protein coding	none	5	616 nt	61 aaENSMUSP00000114807.3	Ready in a moment
ENSMUST00000147709.2	Dio1-207	nonsense mediated decay	none	3	431 nt	82 aaENSMUSP00000121450.2	Ready in a moment
ENSMUST00000106748.2	Dio1-202	protein coding	none	2	396 nt	113 aaENSMUSP00000102359.2	Ready in a moment
ENSMUST00000145332.2	Dio1-206	protein coding cds not defined	none	2	565 nt	none	Ready in a moment

Dio1

What Dio1 does

The RNA a design targets

Where Dio1 is expressed

The protein Dio1 encodes

Proteins STRING associates with Dio1

Where Dio1 acts, as Reactome curates it

Diseases linked to Dio1

Classified variants of Dio1

How much variation Dio1 tolerates

The same gene in mouse, rat and human

MicroRNAs hosted within Dio1

Long non-coding RNAs at the Dio1 locus

Papers that mention Dio1

From Dio1 to a sequence that silences it

Choose the region

Cover the isoforms you mean

Think across species early

Check expression in your model

Run the controls

Pick the product

Dio1

What Dio1 does

The RNA a design targets

Where Dio1 is expressed

The protein Dio1 encodes

Proteins STRING associates with Dio1

Where Dio1 acts, as Reactome curates it

Diseases linked to Dio1

Classified variants of Dio1

How much variation Dio1 tolerates

The same gene in mouse, rat and human

MicroRNAs hosted within Dio1

Long non-coding RNAs at the Dio1 locus

Papers that mention Dio1

Dio1

What Dio1 does

Gene summary

Protein function

The RNA a design targets

RefSeq 1 transcript

A window on one transcript

Ensembl 8 transcripts

A window on one transcript

Where Dio1 is expressed

The protein Dio1 encodes

Proteins STRING associates with Dio1

Where Dio1 acts, as Reactome curates it

Diseases linked to Dio1

Classified variants of Dio1

How much variation Dio1 tolerates

The same gene in mouse, rat and human

MicroRNAs hosted within Dio1

Long non-coding RNAs at the Dio1 locus

Papers that mention Dio1

From Dio1 to a sequence that silences it

Choose the region

Cover the isoforms you mean

Think across species early

Check expression in your model

Run the controls

Pick the product

Gene summary

Protein function

RefSeq 1 transcript

A window on one transcript

Ensembl 8 transcripts

A window on one transcript