Reading the record for Epb41 from NCBI Gene and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 115 seconds for it, and its scripts then bring in the page, or a line saying what did not arrive.
The full name, the identifiers, the location and the notes from the sources arrive with the record. Nothing is filled in ahead of it.
Order door
The door to order for Epb41 opens with the record, which decides which product it carries. The order page itself is open now.
HGNC and MANE Select: not available for mouse. HGNC names human genes; MGI is the authority here, and MANE Select is defined for human transcripts only.
Location
Cytogenetic band 4 D2.3NCBI: 4:131,650,724-131,802,824 on the minus strand, GRCm39 (GCF_000001635.27), sequence NC_000070.7, annotation GCF_000001635.27-RS_2024_02 of 2024-02-01Ensembl: 4:131,650,724-131,802,632 on the minus strand, GRCm39 (GCA_000001635.9), release 116Coordinates are one-based with both ends included, as each source reports them.
Also known as
4.1R, Elp1, Elp-1, Epb4.1, mKIAA4056, D4Ertd442e
Gene identity, from NCBI Datasets · NCBI Datasets 18.37.0 · read · MGI:95401Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
Silence this gene
The order page opens with Epb41 and mouse filled in. The sequences are designed against the transcripts below; the price is on that page.
Predicted to enable 1-phosphatidylinositol binding activity; cytoskeletal…
NCBI Gene summary
Ready in a moment
Reading NCBI Gene and UniProt.Still reading. A first read of a gene can take a while; this page waits up to 30 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
02 / Transcripts and isoforms
The RNA a design targets
44 RefSeq and 33 Ensembl transcripts on GRCm39; RefSeq Select NM_183428.3; Ensembl canonical ENSMUST00000483956.1.
NCBI Datasets · Ensembl
Ready in a moment
Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
03 / Expression by tissue
Where Epb41 is expressed
Expression by tissue: not available for mouse. GTEx holds human tissues only, and the Human Protein Atlas is keyed on human genes.
Ready in a moment
Reading GTEx and the Human Protein Atlas.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
04 / Protein
The protein Epb41 encodes
Reading UniProt, InterPro, AlphaFold DB and PDBe.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
05 / Interactions
Proteins STRING associates with Epb41
Reading STRING.Still reading. A first read of a gene can take a while; this page waits up to 50 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
06 / Pathways
Where Epb41 acts, as Reactome curates it
0 curated, 1 inferred Reactome pathways for P48193 in mouse, v97.
Reactome
Ready in a moment
07 / Disease associations
Diseases linked to Epb41
Disease associations: not available for mouse. Open Targets and ClinGen curate human genes only. The human ortholog's page carries the associations.
Ready in a moment
Not available
Disease associations: not available for mouse. Open Targets and ClinGen curate human genes only. The human ortholog's page carries the associations.
08 / Variants
Classified variants of Epb41
Clinical variants: not available for mouse. ClinVar holds human variants only.
Ready in a moment
Not available
Clinical variants: not available for mouse. ClinVar holds human variants only.
09 / Constraint
How much variation Epb41 tolerates
Constraint: not available for mouse. gnomAD and Open Targets carry constraint for human genes only.
Ready in a moment
Not available
Constraint: not available for mouse. gnomAD and Open Targets carry constraint for human genes only.
10 / Orthologs
The same gene in mouse, rat and human
Reading the Alliance, NCBI, Ensembl Compara and RGD.Still reading. A first read of a gene can take a while; this page waits up to 145 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
11 /MicroRNAs
MicroRNAs hosted within Epb41
Reading Ensembl and miRBase.Still reading. A first read of a gene can take a while; this page waits up to 110 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
12 / Long non-coding RNAs
Long non-coding RNAs at the Epb41 locus
Reading Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 110 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
13 / Literature
Papers that mention Epb41
Reading Europe PMC.Still reading. A first read of a gene can take a while; this page waits up to 55 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
14 / Silencing this gene
From Epb41 to a sequence that silences it
The AUMsilence™ platform designs the sequences against the mouse transcripts on this page. Six decisions are yours before it does. What is written under each is AUM's guidance; the timings and the concentrations are in the usage guide below.
01
Choose the region
A knockdown oligonucleotide can sit in the 5' untranslated region, the coding sequence or the 3' untranslated region, and all three are used. The coding sequence and the 3' untranslated region are the usual first choices for an RNase H design; the 5' end near the start codon suits a steric block. The map above shows where each region sits on the isoforms it draws.
02
Cover the isoforms you mean
An exon every isoform carries silences the whole gene; an exon only some isoforms carry silences those and spares the rest. Decide which you want before a sequence is chosen, and check the reference transcript (MANE Select in human; RefSeq Select and Ensembl canonical in mouse and rat) is the one your cells express.
03
Think across species early
The orthologs panel says whether mouse and rat carry the same gene. Whether one oligonucleotide can serve two species is a sequence question, settled at design by matching the candidate against each transcript, not by the protein identity shown there.
04
Check expression in your model
A transcript that is not expressed in your cells cannot show knockdown. Confirm the gene is expressed in the cell type and condition you will use, from your own data or a reference atlas, before the order. The expression panel above gives GTEx's median per tissue for a human gene; for mouse and rat it says that no atlas is on this page yet.
05
Run the controls
A scramble control of the same chemistry, a positive control against a gene known to knock down in your cells, untreated cells, and a mock condition where a transfection reagent is used. Read knockdown at the RNA level first, then at the protein; the usage guide gives the timing and the concentrations to start from.
06
Pick the product
AUMsilence sdASO needs no transfection reagent and works in the cells that resist one. AUMsilence toASO is the transfection-optimised version of the same design, and AUMsiRNA™ is the siRNA route. The selection guide compares them.
For research use only. Not for use in diagnostic or therapeutic procedures.
Gene summary
Predicted to enable 1-phosphatidylinositol binding activity; cytoskeletal protein binding activity; and phosphoprotein binding activity. Predicted to be a structural constituent of cytoskeleton. Involved in regulation of calcium ion transport and regulation of intestinal absorption. Located in basolateral plasma membrane and cortical cytoskeleton. Is expressed in several structures, including alimentary system; brain; connective tissue; genitourinary system; and retina. Used to study hereditary elliptocytosis. Orthologous to human EPB41 (erythrocyte membrane protein band 4.1).
Provided by Alliance of Genome Resources, Jun 2026, through NCBI Gene. NCBI disclaimer
Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase
NCBI Gene summary · NCBI Gene annotation RS_2024_02 · read · NCBI Gene 269587Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
UniProtKB function · 2026_03 · read · UniProt P48193UniProt data are available under the Creative Commons Attribution 4.0 licence.
Placed on GRCm39 (GCF_000001635.27). MANE Select: not available for mouse. The reference here is the RefSeq Select transcript and the Ensembl canonical transcript, which need not be the same model.
RefSeq 44 transcripts
coding sequence, tall
untranslated region, thin
non-coding exon
intron, fixed width
Genomic strand: minus. Drawn 5' to 3', so exon 1 sits at the left here and at the highest coordinate on the chromosome.
NM_183428.3NM_183428.3RefSeq Select
XM_036164156.1XM_036164156.1
XM_036164157.1XM_036164157.1
XM_036164158.1XM_036164158.1
XM_036164159.1XM_036164159.1
XM_036164160.1XM_036164160.1
XM_006538913.5XM_006538913.5
XM_006538915.5XM_006538915.5
XM_006538916.5XM_006538916.5
XM_036164152.1XM_036164152.1
XM_036164153.1XM_036164153.1
XM_006538893.5XM_006538893.5
XM_006538895.5XM_006538895.5
XM_006538894.5XM_006538894.5
XM_006538907.5XM_006538907.5
XM_036164144.1XM_036164144.1
XM_006538896.5XM_006538896.5
XM_006538897.5XM_006538897.5
XM_006538899.5XM_006538899.5
XM_036164143.1XM_036164143.1
XM_006538900.5XM_006538900.5
XM_017320237.3XM_017320237.3
XM_006538901.5XM_006538901.5
XM_006538902.5XM_006538902.5
XM_036164146.1XM_036164146.1
XM_017320238.3XM_017320238.3
XM_036164148.1XM_036164148.1
XM_017320239.1XM_017320239.1
XM_036164145.1XM_036164145.1
XM_006538908.5XM_006538908.5
XM_030253586.2XM_030253586.2
XM_036164151.1XM_036164151.1
XM_006538909.5XM_006538909.5
XM_006538910.5XM_006538910.5
XM_036164147.1XM_036164147.1
XM_017320241.3XM_017320241.3
XM_036164149.1XM_036164149.1
XM_036164154.1XM_036164154.1
XM_036164155.1XM_036164155.1
XM_006538912.5XM_006538912.5
XM_017320243.2XM_017320243.2
XM_036164150.1XM_036164150.1
NM_001128606.1NM_001128606.1
NM_001128607.2NM_001128607.2
scale
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other, except 10 blocks too short to see, widened to a fixed few pixels; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other, except 13 blocks too short to see, widened to a fixed few pixels; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
Lengths are spliced lengths, as each source states them. Exon ranks follow the strand: on a minus-strand gene exon 1 has the highest genomic coordinate. Reference assembly for mouse: GRCm39.
Expression by tissue: not available for mouse. GTEx holds human tissues only, and the Human Protein Atlas is keyed on human genes.
Mouse tissue expression, from Bgee and Expression Atlas, joins this page in a later phase. Nothing from the human ortholog is shown in its place; its own page is a step away through the species switch above.
1 microRNA gene overlaps Epb41 in Ensembl release 116: Gm55197.
1,972 PubMed-indexed papers mention Epb41 at Europe PMC, newest first. Europe PMC ignores letter case, so the count also covers the human symbol where it differs from this one only in case.
Europe PMC
858 residues, reviewed P48193; 33 entries from the member databases this page shows along the chain; mean pLDDT 63.06; no experimental structure at PDBe.
UniProt · InterPro · AlphaFold DB · PDBe
The 25 highest-scoring STRING partners at or above a combined score of 0.4, of up to 25 asked for; Gypc, Ank1, Ank2 lead.
STRING v12.0
Human EPB41 by 3 of 3 votes; rat Epb41 by 3 of 3 votes. RGD is not answering, so the ortholog list could not be shown. Try again later. Reference ee067b37-954.
