Gene Mouse Mus musculus

Map3k8

mitogen-activated protein kinase kinase kinase 8

protein coding

HumanMouse Map3k8Rat

Reading the ortholog votes for human and rat.

HGNC and MANE Select: not available for mouse. HGNC names human genes; MGI is the authority here, and MANE Select is defined for human transcripts only.

Location: Cytogenetic band 18 A1NCBI: 18:4,331,325-4,352,978 on the minus strand, GRCm39 (GCF_000001635.27), sequence NC_000084.7, annotation GCF_000001635.27-RS_2024_02 of 2024-02-01Ensembl: no location was read.Coordinates are one-based with both ends included, as each source reports them.
Also known as: Cot, Est, Estf, Tpl2, Tpl-2, c-COT, Cot/Tpl2

Gene identity, from NCBI Datasets · NCBI Datasets 18.38.0 · read 2026-09-29 ET · MGI:1346878Data from NCBI, provided as is; NCBI's policies and disclaimers apply.

Notes from the sources

Ensembl did not answer (upstream error).
Ensembl did not answer (upstream error); the Ensembl location and release are missing

Silence this gene

The order page opens with Map3k8 and mouse filled in. The sequences are designed against the transcripts below; the price is on that page.

Order for Map3k8

sdASO, toASO or siRNA, chosen on the next page

Bring your own sequence Transcripts

For research use only. Not for use in diagnostic or therapeutic procedures.

Summary
Transcripts
Expression
Protein
Interactions
Pathways
Disease
Variants
Constraint
Orthologs
MicroRNAs
Long non-coding RNAs
Literature
Silencing this gene

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Ready in a moment

01 Summary and function

What Map3k8 does

Predicted to enable MAP kinase kinase kinase activity.

NCBI Gene summary

Ready in a moment

02 Transcripts and isoforms

The RNA a design targets

Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

Ready in a moment

03 Expression by tissue

Where Map3k8 is expressed

Expression by tissue: not available for mouse. GTEx holds human tissues only, and the Human Protein Atlas is keyed on human genes.

Ready in a moment

04 Protein

The protein Map3k8 encodes

Reading UniProt, InterPro, AlphaFold DB and PDBe.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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05 Interactions

Proteins STRING associates with Map3k8

Reading STRING.Still reading. A first read of a gene can take a while; this page waits up to 50 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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06 Pathways

Where Map3k8 acts, as Reactome curates it

Reading UniProt and Reactome.Still reading. A first read of a gene can take a while; this page waits up to 60 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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07 Disease associations

Diseases linked to Map3k8

Disease associations: not available for mouse. Open Targets and ClinGen curate human genes only. The human ortholog's page carries the associations.

Ready in a moment

08 Variants

Classified variants of Map3k8

Clinical variants: not available for mouse. ClinVar holds human variants only.

Ready in a moment

09 Constraint

How much variation Map3k8 tolerates

Constraint: not available for mouse. gnomAD and Open Targets carry constraint for human genes only.

Ready in a moment

10 Orthologs

The same gene in mouse, rat and human

Reading the Alliance, NCBI, Ensembl Compara and RGD.Still reading. A first read of a gene can take a while; this page waits up to 145 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

Ready in a moment

11 MicroRNAs

MicroRNAs hosted within Map3k8

Ensembl is not answering, so the non-coding annotation could not be shown. Try again later. Reference e27153ae-f9b.

Ready in a moment

12 Long non-coding RNAs

Long non-coding RNAs at the Map3k8 locus

Ensembl is not answering, so the non-coding annotation could not be shown. Try again later. Reference e27153ae-f9b.

Ready in a moment

13 Literature

Papers that mention Map3k8

Reading Europe PMC.Still reading. A first read of a gene can take a while; this page waits up to 55 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

Ready in a moment

14 Silencing this gene

From Map3k8 to a sequence that silences it

The AUMsilence platform designs the sequences against the mouse transcripts on this page. Six decisions are yours before it does. What is written under each is AUM's guidance; the timings and the concentrations are in the usage guide below.

01
Choose the region
A knockdown oligonucleotide can sit in the 5' untranslated region, the coding sequence or the 3' untranslated region, and all three are used. The coding sequence and the 3' untranslated region are the usual first choices for an RNase H design; the 5' end near the start codon suits a steric block. The map above shows where each region sits on the isoforms it draws.
02
Cover the isoforms you mean
An exon every isoform carries silences the whole gene; an exon only some isoforms carry silences those and spares the rest. Decide which you want before a sequence is chosen, and check the reference transcript (MANE Select in human; RefSeq Select and Ensembl canonical in mouse and rat) is the one your cells express.
03
Think across species early
The orthologs panel says whether mouse and rat carry the same gene. Whether one oligonucleotide can serve two species is a sequence question, settled at design by matching the candidate against each transcript, not by the protein identity shown there.
04
Check expression in your model
A transcript that is not expressed in your cells cannot show knockdown. Confirm the gene is expressed in the cell type and condition you will use, from your own data or a reference atlas, before the order. The expression panel above gives GTEx's median per tissue for a human gene; for mouse and rat it says that no atlas is on this page yet.
05
Run the controls
A scramble control of the same chemistry, a positive control against a gene known to knock down in your cells, untreated cells, and a mock condition where a transfection reagent is used. Read knockdown at the RNA level first, then at the protein; the usage guide gives the timing and the concentrations to start from.
06
Pick the product
AUMsilence sdASO needs no transfection reagent and works in the cells that resist one. AUMsilence toASO is the transfection-optimised version of the same design, and AUMsiRNA is the siRNA route. The selection guide compares them.

Order for Map3k8 Bring your own sequence The usage guide Selection guide

For research use only. Not for use in diagnostic or therapeutic procedures.

Gene Mouse Mus musculus

Map3k8

Reading the record for Map3k8 from NCBI Gene and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 115 seconds for it, and its scripts then bring in the page, or a line saying what did not arrive.

The full name, the identifiers, the location and the notes from the sources arrive with the record. Nothing is filled in ahead of it.

Order door

The door to order for Map3k8 opens with the record, which decides which product it carries. The order page itself is open now.

Bring your own sequence

For research use only. Not for use in diagnostic or therapeutic procedures.

This page is still reading the record. Its scripts replace this shell with the record when it arrives; with scripts off, it stays at this shell however long you wait.

01 Summary and function

What Map3k8 does

Waiting for the record before reading NCBI Gene and UniProt.

02 Transcripts and isoforms

The RNA a design targets

Waiting for the record before reading NCBI Datasets and Ensembl.

03 Expression by tissue

Where Map3k8 is expressed

Waiting for the record before reading GTEx and the Human Protein Atlas.

04 Protein

The protein Map3k8 encodes

Waiting for the record before reading UniProt, InterPro, AlphaFold DB and PDBe.

05 Interactions

Proteins STRING associates with Map3k8

Waiting for the record before reading STRING.

06 Pathways

Where Map3k8 acts, as Reactome curates it

Waiting for the record before reading UniProt and Reactome.

07 Disease associations

Diseases linked to Map3k8

Waiting for the record before reading Open Targets and ClinGen.

08 Variants

Classified variants of Map3k8

Waiting for the record before reading ClinVar.

09 Constraint

How much variation Map3k8 tolerates

Waiting for the record before reading gnomAD and Open Targets.

10 Orthologs

The same gene in mouse, rat and human

Waiting for the record before reading the Alliance, NCBI, Ensembl Compara and RGD.

11 MicroRNAs

MicroRNAs hosted within Map3k8

Waiting for the record before reading Ensembl and miRBase.

12 Long non-coding RNAs

Long non-coding RNAs at the Map3k8 locus

Waiting for the record before reading Ensembl.

13 Literature

Papers that mention Map3k8

Waiting for the record before reading Europe PMC.

Accession	Name	Biotype	Labels	Exons	Spliced length	Protein	Sequence
NM_007746.2matches ENSMUST00000025078.11	transcript variant 1	protein coding	RefSeq Select	8	2,507 nt	467 aaNP_031772.1	Ready in a moment
XM_006525944.3	transcript variant X2	protein coding model	none	8	2,626 nt	310 aaXP_006526007.1	Ready in a moment
XM_036161157.1	transcript variant X3	protein coding model	none	7	2,364 nt	290 aaXP_036017050.1	Ready in a moment
XM_036161158.1	transcript variant X4	protein coding model	none	Ready in a moment

Accession	Name	Biotype	Labels	Exons	Spliced length	Protein	Sequence
NM_007746.2matches ENSMUST00000025078.11	transcript variant 1	protein coding	RefSeq Select	8	2,507 nt	467 aaNP_031772.1	Ready in a moment
XM_006525944.3	transcript variant X2	protein coding model	none	8	2,626 nt	310 aaXP_006526007.1	Ready in a moment
XM_036161157.1	transcript variant X3	protein coding model	none	7	2,364 nt	290 aaXP_036017050.1	Ready in a moment
XM_036161158.1	transcript variant X4	protein coding model	none	Ready in a moment

Map3k8

What Map3k8 does

The RNA a design targets

Where Map3k8 is expressed

The protein Map3k8 encodes

Proteins STRING associates with Map3k8

Where Map3k8 acts, as Reactome curates it

Diseases linked to Map3k8

Classified variants of Map3k8

How much variation Map3k8 tolerates

The same gene in mouse, rat and human

MicroRNAs hosted within Map3k8

Long non-coding RNAs at the Map3k8 locus

Papers that mention Map3k8

From Map3k8 to a sequence that silences it

Choose the region

Cover the isoforms you mean

Think across species early

Check expression in your model

Run the controls

Pick the product

Map3k8

What Map3k8 does

The RNA a design targets

Where Map3k8 is expressed

The protein Map3k8 encodes

Proteins STRING associates with Map3k8

Where Map3k8 acts, as Reactome curates it

Diseases linked to Map3k8

Classified variants of Map3k8

How much variation Map3k8 tolerates

The same gene in mouse, rat and human

MicroRNAs hosted within Map3k8

Long non-coding RNAs at the Map3k8 locus

Papers that mention Map3k8

Map3k8

What Map3k8 does

Gene summary

Protein function

The RNA a design targets

RefSeq 6 transcripts

A window on one transcript

Ensembl 0 transcripts

Where Map3k8 is expressed

The protein Map3k8 encodes

Proteins STRING associates with Map3k8

Where Map3k8 acts, as Reactome curates it

Diseases linked to Map3k8

Classified variants of Map3k8

How much variation Map3k8 tolerates

The same gene in mouse, rat and human

MicroRNAs hosted within Map3k8

Long non-coding RNAs at the Map3k8 locus

Papers that mention Map3k8

From Map3k8 to a sequence that silences it

Choose the region

Cover the isoforms you mean

Think across species early

Check expression in your model

Run the controls

Pick the product

Gene summary

Protein function

RefSeq 6 transcripts

A window on one transcript

Ensembl 0 transcripts