Reading the record for Ndfip1 from NCBI Gene and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 115 seconds for it, and its scripts then bring in the page, or a line saying what did not arrive.
The full name, the identifiers, the location and the notes from the sources arrive with the record. Nothing is filled in ahead of it.
Order door
The door to order for Ndfip1 opens with the record, which decides which product it carries. The order page itself is open now.
HGNC and MANE Select: not available for mouse. HGNC names human genes; MGI is the authority here, and MANE Select is defined for human transcripts only.
Location
Cytogenetic band 18 B3NCBI: 18:38,551,960-38,598,250 on the plus strand, GRCm39 (GCF_000001635.27), sequence NC_000084.7, annotation GCF_000001635.27-RS_2024_02 of 2024-02-01Ensembl: 18:38,543,449-38,599,910 on the plus strand, GRCm39 (GCA_000001635.9), release 116Coordinates are one-based with both ends included, as each source reports them.
Also known as
N4wbp5, 0610010M22Rik
Gene identity, from NCBI Datasets · NCBI Datasets 18.38.0 · read · MGI:1929601Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
Silence this gene
The order page opens with Ndfip1 and mouse filled in. The sequences are designed against the transcripts below; the price is on that page.
Reading NCBI Gene and UniProt.Still reading. A first read of a gene can take a while; this page waits up to 30 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
02 / Transcripts and isoforms
The RNA a design targets
Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
03 / Expression by tissue
Where Ndfip1 is expressed
Expression by tissue: not available for mouse. GTEx holds human tissues only, and the Human Protein Atlas is keyed on human genes.
Ready in a moment
Reading GTEx and the Human Protein Atlas.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
04 / Protein
The protein Ndfip1 encodes
Reading UniProt, InterPro, AlphaFold DB and PDBe.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
05 / Interactions
Proteins STRING associates with Ndfip1
Reading STRING.Still reading. A first read of a gene can take a while; this page waits up to 50 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
06 / Pathways
Where Ndfip1 acts, as Reactome curates it
Reading UniProt and Reactome.Still reading. A first read of a gene can take a while; this page waits up to 60 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
07 / Disease associations
Diseases linked to Ndfip1
Disease associations: not available for mouse. Open Targets and ClinGen curate human genes only. The human ortholog's page carries the associations.
Ready in a moment
Not available
Disease associations: not available for mouse. Open Targets and ClinGen curate human genes only. The human ortholog's page carries the associations.
08 / Variants
Classified variants of Ndfip1
Clinical variants: not available for mouse. ClinVar holds human variants only.
Ready in a moment
Not available
Clinical variants: not available for mouse. ClinVar holds human variants only.
09 / Constraint
How much variation Ndfip1 tolerates
Constraint: not available for mouse. gnomAD and Open Targets carry constraint for human genes only.
Ready in a moment
Not available
Constraint: not available for mouse. gnomAD and Open Targets carry constraint for human genes only.
10 / Orthologs
The same gene in mouse, rat and human
Reading the Alliance, NCBI, Ensembl Compara and RGD.Still reading. A first read of a gene can take a while; this page waits up to 145 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
11 /MicroRNAs
MicroRNAs hosted within Ndfip1
Reading Ensembl and miRBase.Still reading. A first read of a gene can take a while; this page waits up to 110 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
12 / Long non-coding RNAs
Long non-coding RNAs at the Ndfip1 locus
Reading Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 110 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
13 / Literature
Papers that mention Ndfip1
Reading Europe PMC.Still reading. A first read of a gene can take a while; this page waits up to 55 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
14 / Silencing this gene
From Ndfip1 to a sequence that silences it
The AUMsilence™ platform designs the sequences against the mouse transcripts on this page. Six decisions are yours before it does. What is written under each is AUM's guidance; the timings and the concentrations are in the usage guide below.
01
Choose the region
A knockdown oligonucleotide can sit in the 5' untranslated region, the coding sequence or the 3' untranslated region, and all three are used. The coding sequence and the 3' untranslated region are the usual first choices for an RNase H design; the 5' end near the start codon suits a steric block. The map above shows where each region sits on the isoforms it draws.
02
Cover the isoforms you mean
An exon every isoform carries silences the whole gene; an exon only some isoforms carry silences those and spares the rest. Decide which you want before a sequence is chosen, and check the reference transcript (MANE Select in human; RefSeq Select and Ensembl canonical in mouse and rat) is the one your cells express.
03
Think across species early
The orthologs panel says whether mouse and rat carry the same gene. Whether one oligonucleotide can serve two species is a sequence question, settled at design by matching the candidate against each transcript, not by the protein identity shown there.
04
Check expression in your model
A transcript that is not expressed in your cells cannot show knockdown. Confirm the gene is expressed in the cell type and condition you will use, from your own data or a reference atlas, before the order. The expression panel above gives GTEx's median per tissue for a human gene; for mouse and rat it says that no atlas is on this page yet.
05
Run the controls
A scramble control of the same chemistry, a positive control against a gene known to knock down in your cells, untreated cells, and a mock condition where a transfection reagent is used. Read knockdown at the RNA level first, then at the protein; the usage guide gives the timing and the concentrations to start from.
06
Pick the product
AUMsilence sdASO needs no transfection reagent and works in the cells that resist one. AUMsilence toASO is the transfection-optimised version of the same design, and AUMsiRNA™ is the siRNA route. The selection guide compares them.
For research use only. Not for use in diagnostic or therapeutic procedures.
Gene summary
Enables WW domain binding activity. Acts upstream of or within several processes, including negative regulation of interleukin-4 production; negative regulation of type 2 immune response; and regulation of lymphocyte activation. Located in Golgi apparatus and cell cortex. Is expressed in alimentary system; nervous system; retina; and rib. Orthologous to human NDFIP1 (Nedd4 family interacting protein 1).
Provided by Alliance of Genome Resources, Jun 2026, through NCBI Gene. NCBI disclaimer
Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cell-mediated inflammation by activating ITCH and thus controlling JUNB degradation (PubMed:11748237, PubMed:17137798, PubMed:20962770). Promotes pancreatic beta cell death through degradation of JUNB and inhibition of the unfolded protein response, leading to reduction of insulin secretion (PubMed:26319551). Restricts the production of pro-inflammatory cytokines in effector Th17 T-cells by promoting ITCH-mediated ubiquitination and degradation of RORC (PubMed:28051111). Together with NDFIP2, limits the cytokine signaling and expansion of effector Th2 T-cells by promoting degradation of JAK1, probably by ITCH- and NEDD4L-mediated ubiquitination (PubMed:27088444). Regulates peripheral T-cell tolerance to self and foreign antigens, forcing the exit of naive CD4+ T-cells from the cell cycle before they become effector T-cells (PubMed:24520172, PubMed:28051111). Negatively regulates RLR-mediated antiviral response by promoting SMURF1-mediated ubiquitination and subsequent degradation of MAVS (By similarity). Negatively regulates KCNH2 potassium channel activity by decreasing its cell-surface expression and interfering with channel maturation through recruitment of NEDD4L to the Golgi apparatus where it mediates KCNH2 degradation (By similarity). In cortical neurons, mediates the ubiquitination of the divalent metal transporter SLC11A2/DMT1 by NEDD4L, leading to its down-regulation and protection of the cells from cobalt and iron toxicity (By similarity). Important for normal development of dendrites and dendritic spines in cortex (PubMed:23897647). Enhances the ubiquitination of BRAT1 mediated by: NEDD4, NEDD4L and ITCH and is required for the nuclear localization of ubiquitinated BRAT1 (PubMed:25631046). Enhances the ITCH-mediated ubiquitination of MAP3K7 by recruiting E2 ubiquitin-conjugating enzyme UBE2L3 to ITCH (PubMed:25632008). Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate (By similarity). Inhibits cell proliferation by promoting PTEN nuclear localization and changing its signaling specificity (PubMed:25801959)
NCBI Gene summary · NCBI Gene annotation RS_2024_02 · read · NCBI Gene 65113Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
UniProtKB function · 2026_03 · read · UniProt Q8R0W6UniProt data are available under the Creative Commons Attribution 4.0 licence.
Expression by tissue: not available for mouse. GTEx holds human tissues only, and the Human Protein Atlas is keyed on human genes.
Mouse tissue expression, from Bgee and Expression Atlas, joins this page in a later phase. Nothing from the human ortholog is shown in its place; its own page is a step away through the species switch above.
No annotated microRNA lies within Ndfip1 in Ensembl release 116, on GRCm39.
Ensembl · miRBase
2 long non-coding RNA genes overlap Ndfip1 in Ensembl release 116, all antisense: Gm58422, Gm58424.
809 PubMed-indexed papers mention Ndfip1 at Europe PMC, newest first. Europe PMC ignores letter case, so the count also covers the human symbol where it differs from this one only in case.
Europe PMC
Reactome v97 maps no pathway to Q8R0W6 in mouse.
Reactome
The 25 highest-scoring STRING partners at or above a combined score of 0.4, of up to 25 asked for; Nedd4, Wwp2, Itch lead.
STRING v12.0
2 RefSeq and 39 Ensembl transcripts on GRCm39; RefSeq Select NM_022996.2; Ensembl canonical ENSMUST00000447522.1.
NCBI Datasets · Ensembl
Placed on GRCm39 (GCF_000001635.27). MANE Select: not available for mouse. The reference here is the RefSeq Select transcript and the Ensembl canonical transcript, which need not be the same model.
RefSeq 2 transcripts
coding sequence, tall
untranslated region, thin
non-coding exon
intron, fixed width
Genomic strand: plus. Drawn 5' to 3', so exon 1 sits at the left here and at the lowest coordinate on the chromosome.
NM_022996.2NM_022996.2RefSeq Select
NM_001355749.1NM_001355749.1
scale
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
NCBI Datasets, RefSeq transcripts · NCBI Datasets 18.38.0; GCF_000001635.27-RS_2024_02 · read · NCBI Gene 65113Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
Ensembl 39 transcripts
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
Lengths are spliced lengths, as each source states them. Exon ranks follow the strand: on a minus-strand gene exon 1 has the highest genomic coordinate. Reference assembly for mouse: GRCm39.
coding sequence, tall
untranslated region, thin
non-coding exon
intron, fixed width
Genomic strand: plus. Drawn 5' to 3', so exon 1 sits at the left here and at the lowest coordinate on the chromosome.
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other, except 1 block too short to see, widened to a fixed few pixels; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
Human NDFIP1 by 3 of 3 votes; rat Ndfip1 by 3 of 3 votes. RGD is not answering, so the ortholog list could not be shown. Try again later. Reference 6dd91c37-171.
Alliance · NCBI · Ensembl Compara
221 residues, reviewed Q8R0W6; 4 entries from the member databases this page shows along the chain; mean pLDDT 62.31; no experimental structure at PDBe.
