Gene Mouse Mus musculus

Ndufaf2

NADH:ubiquinone oxidoreductase complex assembly factor 2

protein coding

HumanMouse Ndufaf2Rat

Reading the ortholog votes for human and rat.

HGNC and MANE Select: not available for mouse. HGNC names human genes; MGI is the authority here, and MANE Select is defined for human transcripts only.

Location: Cytogenetic band 13 D2.1NCBI: 13:108,189,123-108,295,189 on the minus strand, GRCm39 (GCF_000001635.27), sequence NC_000079.7, annotation GCF_000001635.27-RS_2024_02 of 2024-02-01Ensembl: 13:108,139,249-108,295,189 on the minus strand, GRCm39 (GCA_000001635.9), release 116Coordinates are one-based with both ends included, as each source reports them.
Also known as: mimitin, Ndufa12l, 1810058I14Rik

Gene identity, from NCBI Datasets · NCBI Datasets 18.38.0 · read 2026-09-29 ET · MGI:1922847Data from NCBI, provided as is; NCBI's policies and disclaimers apply.

Silence this gene

The order page opens with Ndufaf2 and mouse filled in. The sequences are designed against the transcripts below; the price is on that page.

Order for Ndufaf2

sdASO, toASO or siRNA, chosen on the next page

Bring your own sequence Transcripts

For research use only. Not for use in diagnostic or therapeutic procedures.

Summary
Transcripts
Expression
Protein
Interactions
Pathways
Disease
Variants
Constraint
Orthologs
MicroRNAs
Long non-coding RNAs
Literature
Silencing this gene

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Ready in a moment

01 Summary and function

What Ndufaf2 does

Acts upstream of or within mitochondrial respiratory chain complex I… UniProt answered in a shape this page does not read, so nothing is shown for the protein entry. Reference ca889528-134.

NCBI Gene summary

Ready in a moment

02 Transcripts and isoforms

The RNA a design targets

Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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03 Expression by tissue

Where Ndufaf2 is expressed

Expression by tissue: not available for mouse. GTEx holds human tissues only, and the Human Protein Atlas is keyed on human genes.

Ready in a moment

04 Protein

The protein Ndufaf2 encodes

UniProt answered in a shape this page does not read, so nothing is shown for the protein entry. Reference ca889528-134.

Ready in a moment

05 Interactions

Proteins STRING associates with Ndufaf2

Reading STRING.Still reading. A first read of a gene can take a while; this page waits up to 50 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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06 Pathways

Where Ndufaf2 acts, as Reactome curates it

UniProt answered in a shape this page does not read, so nothing is shown for the pathway list. Reference ca889528-134.

Ready in a moment

07 Disease associations

Diseases linked to Ndufaf2

Disease associations: not available for mouse. Open Targets and ClinGen curate human genes only. The human ortholog's page carries the associations.

Ready in a moment

08 Variants

Classified variants of Ndufaf2

Clinical variants: not available for mouse. ClinVar holds human variants only.

Ready in a moment

09 Constraint

How much variation Ndufaf2 tolerates

Constraint: not available for mouse. gnomAD and Open Targets carry constraint for human genes only.

Ready in a moment

10 Orthologs

The same gene in mouse, rat and human

Reading the Alliance, NCBI, Ensembl Compara and RGD.Still reading. A first read of a gene can take a while; this page waits up to 145 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

Ready in a moment

11 MicroRNAs

MicroRNAs hosted within Ndufaf2

Reading Ensembl and miRBase.Still reading. A first read of a gene can take a while; this page waits up to 110 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

Ready in a moment

12 Long non-coding RNAs

Long non-coding RNAs at the Ndufaf2 locus

Reading Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 110 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

Ready in a moment

13 Literature

Papers that mention Ndufaf2

Reading Europe PMC.Still reading. A first read of a gene can take a while; this page waits up to 55 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

Ready in a moment

14 Silencing this gene

From Ndufaf2 to a sequence that silences it

The AUMsilence platform designs the sequences against the mouse transcripts on this page. Six decisions are yours before it does. What is written under each is AUM's guidance; the timings and the concentrations are in the usage guide below.

01
Choose the region
A knockdown oligonucleotide can sit in the 5' untranslated region, the coding sequence or the 3' untranslated region, and all three are used. The coding sequence and the 3' untranslated region are the usual first choices for an RNase H design; the 5' end near the start codon suits a steric block. The map above shows where each region sits on the isoforms it draws.
02
Cover the isoforms you mean
An exon every isoform carries silences the whole gene; an exon only some isoforms carry silences those and spares the rest. Decide which you want before a sequence is chosen, and check the reference transcript (MANE Select in human; RefSeq Select and Ensembl canonical in mouse and rat) is the one your cells express.
03
Think across species early
The orthologs panel says whether mouse and rat carry the same gene. Whether one oligonucleotide can serve two species is a sequence question, settled at design by matching the candidate against each transcript, not by the protein identity shown there.
04
Check expression in your model
A transcript that is not expressed in your cells cannot show knockdown. Confirm the gene is expressed in the cell type and condition you will use, from your own data or a reference atlas, before the order. The expression panel above gives GTEx's median per tissue for a human gene; for mouse and rat it says that no atlas is on this page yet.
05
Run the controls
A scramble control of the same chemistry, a positive control against a gene known to knock down in your cells, untreated cells, and a mock condition where a transfection reagent is used. Read knockdown at the RNA level first, then at the protein; the usage guide gives the timing and the concentrations to start from.
06
Pick the product
AUMsilence sdASO needs no transfection reagent and works in the cells that resist one. AUMsilence toASO is the transfection-optimised version of the same design, and AUMsiRNA is the siRNA route. The selection guide compares them.

Order for Ndufaf2 Bring your own sequence The usage guide Selection guide

For research use only. Not for use in diagnostic or therapeutic procedures.

Gene Mouse Mus musculus

Ndufaf2

Reading the record for Ndufaf2 from NCBI Gene and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 115 seconds for it, and its scripts then bring in the page, or a line saying what did not arrive.

The full name, the identifiers, the location and the notes from the sources arrive with the record. Nothing is filled in ahead of it.

Order door

The door to order for Ndufaf2 opens with the record, which decides which product it carries. The order page itself is open now.

Bring your own sequence

For research use only. Not for use in diagnostic or therapeutic procedures.

This page is still reading the record. Its scripts replace this shell with the record when it arrives; with scripts off, it stays at this shell however long you wait.

01 Summary and function

What Ndufaf2 does

Waiting for the record before reading NCBI Gene and UniProt.

02 Transcripts and isoforms

The RNA a design targets

Waiting for the record before reading NCBI Datasets and Ensembl.

03 Expression by tissue

Where Ndufaf2 is expressed

Waiting for the record before reading GTEx and the Human Protein Atlas.

04 Protein

The protein Ndufaf2 encodes

Waiting for the record before reading UniProt, InterPro, AlphaFold DB and PDBe.

05 Interactions

Proteins STRING associates with Ndufaf2

Waiting for the record before reading STRING.

06 Pathways

Where Ndufaf2 acts, as Reactome curates it

Waiting for the record before reading UniProt and Reactome.

07 Disease associations

Diseases linked to Ndufaf2

Waiting for the record before reading Open Targets and ClinGen.

08 Variants

Classified variants of Ndufaf2

Waiting for the record before reading ClinVar.

09 Constraint

How much variation Ndufaf2 tolerates

Waiting for the record before reading gnomAD and Open Targets.

10 Orthologs

The same gene in mouse, rat and human

Waiting for the record before reading the Alliance, NCBI, Ensembl Compara and RGD.

11 MicroRNAs

MicroRNAs hosted within Ndufaf2

Waiting for the record before reading Ensembl and miRBase.

12 Long non-coding RNAs

Long non-coding RNAs at the Ndufaf2 locus

Waiting for the record before reading Ensembl.

13 Literature

Papers that mention Ndufaf2

Waiting for the record before reading Europe PMC.

Accession	Name	Biotype	Labels	Exons	Spliced length	Protein	Sequence
NM_001127346.2matches ENSMUST00000163558.4	transcript variant 1	protein coding	RefSeq Select	4	646 nt	168 aaNP_001120818.1	Ready in a moment
NM_001360140.2matches ENSMUST00000561861.1	transcript variant 2	protein coding	none	5	744 nt	102 aaNP_001347069.1	Ready in a moment
XM_036158205.1	transcript variant X1	protein coding model	none	5	678 nt	168 aaXP_036014098.1	Ready in a moment
XM_030247443.2matches ENSMUST00000223734.3	Ready in a moment

Accession	Name	Biotype	Labels	Exons	Spliced length	Protein	Sequence
NM_001127346.2matches ENSMUST00000163558.4	transcript variant 1	protein coding	RefSeq Select	4	646 nt	168 aaNP_001120818.1	Ready in a moment
NM_001360140.2matches ENSMUST00000561861.1	transcript variant 2	protein coding	none	5	744 nt	102 aaNP_001347069.1	Ready in a moment
XM_036158205.1	transcript variant X1	protein coding model	none	5	678 nt	168 aaXP_036014098.1	Ready in a moment
XM_030247443.2matches ENSMUST00000223734.3	Ready in a moment

Ndufaf2

What Ndufaf2 does

The RNA a design targets

Where Ndufaf2 is expressed

The protein Ndufaf2 encodes

Proteins STRING associates with Ndufaf2

Where Ndufaf2 acts, as Reactome curates it

Diseases linked to Ndufaf2

Classified variants of Ndufaf2

How much variation Ndufaf2 tolerates

The same gene in mouse, rat and human

MicroRNAs hosted within Ndufaf2

Long non-coding RNAs at the Ndufaf2 locus

Papers that mention Ndufaf2

From Ndufaf2 to a sequence that silences it

Choose the region

Cover the isoforms you mean

Think across species early

Check expression in your model

Run the controls

Pick the product

Ndufaf2

What Ndufaf2 does

The RNA a design targets

Where Ndufaf2 is expressed

The protein Ndufaf2 encodes

Proteins STRING associates with Ndufaf2

Where Ndufaf2 acts, as Reactome curates it

Diseases linked to Ndufaf2

Classified variants of Ndufaf2

How much variation Ndufaf2 tolerates

The same gene in mouse, rat and human

MicroRNAs hosted within Ndufaf2

Long non-coding RNAs at the Ndufaf2 locus

Papers that mention Ndufaf2

Ndufaf2

What Ndufaf2 does

Gene summary

Protein function

The RNA a design targets

RefSeq 5 transcripts

A window on one transcript

Ensembl 0 transcripts

Where Ndufaf2 is expressed

The protein Ndufaf2 encodes

Proteins STRING associates with Ndufaf2

Where Ndufaf2 acts, as Reactome curates it

Diseases linked to Ndufaf2

Classified variants of Ndufaf2

How much variation Ndufaf2 tolerates

The same gene in mouse, rat and human

MicroRNAs hosted within Ndufaf2

Long non-coding RNAs at the Ndufaf2 locus

Papers that mention Ndufaf2

From Ndufaf2 to a sequence that silences it

Choose the region

Cover the isoforms you mean

Think across species early

Check expression in your model

Run the controls

Pick the product

Gene summary

Protein function

RefSeq 5 transcripts

A window on one transcript

Ensembl 0 transcripts