Reading the record for Nt5c3b from NCBI Gene and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 115 seconds for it, and its scripts then bring in the page, or a line saying what did not arrive.
The full name, the identifiers, the location and the notes from the sources arrive with the record. Nothing is filled in ahead of it.
Order door
The door to order for Nt5c3b opens with the record, which decides which product it carries. The order page itself is open now.
HGNC and MANE Select: not available for mouse. HGNC names human genes; MGI is the authority here, and MANE Select is defined for human transcripts only.
Location
Cytogenetic band 11 DNCBI: 11:100,313,143-100,332,634 on the minus strand, GRCm39 (GCF_000001635.27), sequence NC_000077.7, annotation GCF_000001635.27-RS_2024_02 of 2024-02-01Ensembl: 11:100,312,280-100,332,634 on the minus strand, GRCm39 (GCA_000001635.9), release 116Coordinates are one-based with both ends included, as each source reports them.
Also known as
Nt5c3l, 2610037D24Rik, C330027I04Rik
Gene identity, from NCBI Datasets · NCBI Datasets 18.38.0 · read · MGI:1915356Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
Silence this gene
The order page opens with Nt5c3b and mouse filled in. The sequences are designed against the transcripts below; the price is on that page.
Predicted to enable magnesium ion binding activity.
NCBI Gene summary
Ready in a moment
Reading NCBI Gene and UniProt.Still reading. A first read of a gene can take a while; this page waits up to 30 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
02 / Transcripts and isoforms
The RNA a design targets
Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
03 / Expression by tissue
Where Nt5c3b is expressed
Expression by tissue: not available for mouse. GTEx holds human tissues only, and the Human Protein Atlas is keyed on human genes.
Ready in a moment
Reading GTEx and the Human Protein Atlas.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
04 / Protein
The protein Nt5c3b encodes
Reading UniProt, InterPro, AlphaFold DB and PDBe.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
05 / Interactions
Proteins STRING associates with Nt5c3b
The 25 highest-scoring STRING partners at or above a combined score of 0.4, of up to 25 asked for; Nt5c1b, Nt5m, Nt5c1a lead.
STRING v12.0
Ready in a moment
06 / Pathways
Where Nt5c3b acts, as Reactome curates it
Reading UniProt and Reactome.Still reading. A first read of a gene can take a while; this page waits up to 60 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
07 / Disease associations
Diseases linked to Nt5c3b
Disease associations: not available for mouse. Open Targets and ClinGen curate human genes only. The human ortholog's page carries the associations.
Ready in a moment
Not available
Disease associations: not available for mouse. Open Targets and ClinGen curate human genes only. The human ortholog's page carries the associations.
08 / Variants
Classified variants of Nt5c3b
Clinical variants: not available for mouse. ClinVar holds human variants only.
Ready in a moment
Not available
Clinical variants: not available for mouse. ClinVar holds human variants only.
09 / Constraint
How much variation Nt5c3b tolerates
Constraint: not available for mouse. gnomAD and Open Targets carry constraint for human genes only.
Ready in a moment
Not available
Constraint: not available for mouse. gnomAD and Open Targets carry constraint for human genes only.
10 / Orthologs
The same gene in mouse, rat and human
Reading the Alliance, NCBI, Ensembl Compara and RGD.Still reading. A first read of a gene can take a while; this page waits up to 145 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
11 /MicroRNAs
MicroRNAs hosted within Nt5c3b
Reading Ensembl and miRBase.Still reading. A first read of a gene can take a while; this page waits up to 110 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
12 / Long non-coding RNAs
Long non-coding RNAs at the Nt5c3b locus
Reading Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 110 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
13 / Literature
Papers that mention Nt5c3b
90 PubMed-indexed papers mention Nt5c3b at Europe PMC, newest first. Europe PMC ignores letter case, so the count also covers the human symbol where it differs from this one only in case.
Europe PMC
Ready in a moment
14 / Silencing this gene
From Nt5c3b to a sequence that silences it
The AUMsilence™ platform designs the sequences against the mouse transcripts on this page. Six decisions are yours before it does. What is written under each is AUM's guidance; the timings and the concentrations are in the usage guide below.
01
Choose the region
A knockdown oligonucleotide can sit in the 5' untranslated region, the coding sequence or the 3' untranslated region, and all three are used. The coding sequence and the 3' untranslated region are the usual first choices for an RNase H design; the 5' end near the start codon suits a steric block. The map above shows where each region sits on the isoforms it draws.
02
Cover the isoforms you mean
An exon every isoform carries silences the whole gene; an exon only some isoforms carry silences those and spares the rest. Decide which you want before a sequence is chosen, and check the reference transcript (MANE Select in human; RefSeq Select and Ensembl canonical in mouse and rat) is the one your cells express.
03
Think across species early
The orthologs panel says whether mouse and rat carry the same gene. Whether one oligonucleotide can serve two species is a sequence question, settled at design by matching the candidate against each transcript, not by the protein identity shown there.
04
Check expression in your model
A transcript that is not expressed in your cells cannot show knockdown. Confirm the gene is expressed in the cell type and condition you will use, from your own data or a reference atlas, before the order. The expression panel above gives GTEx's median per tissue for a human gene; for mouse and rat it says that no atlas is on this page yet.
05
Run the controls
A scramble control of the same chemistry, a positive control against a gene known to knock down in your cells, untreated cells, and a mock condition where a transfection reagent is used. Read knockdown at the RNA level first, then at the protein; the usage guide gives the timing and the concentrations to start from.
06
Pick the product
AUMsilence sdASO needs no transfection reagent and works in the cells that resist one. AUMsilence toASO is the transfection-optimised version of the same design, and AUMsiRNA™ is the siRNA route. The selection guide compares them.
For research use only. Not for use in diagnostic or therapeutic procedures.
Gene summary
Predicted to enable magnesium ion binding activity. Predicted to be active in cytoplasm. Orthologous to human NT5C3B (5'-nucleotidase, cytosolic IIIB).
Provided by Alliance of Genome Resources, Jun 2026, through NCBI Gene. NCBI disclaimer
Specifically hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m(7)GMP may protect cells against undesired salvage of m(7)GMP and its incorporation into nucleic acids. Also has weak activity for CMP. UMP and purine nucleotides are poor substrates (By similarity)
NCBI Gene summary · NCBI Gene annotation RS_2024_02 · read · NCBI Gene 68106Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
UniProtKB function · 2026_03 · read · UniProt Q3UFY7UniProt data are available under the Creative Commons Attribution 4.0 licence.
Expression by tissue: not available for mouse. GTEx holds human tissues only, and the Human Protein Atlas is keyed on human genes.
Mouse tissue expression, from Bgee and Expression Atlas, joins this page in a later phase. Nothing from the human ortholog is shown in its place; its own page is a step away through the species switch above.
No annotated microRNA lies within Nt5c3b in Ensembl release 116, on GRCm39.
Ensembl · miRBase
1 long non-coding RNA gene overlaps Nt5c3b in Ensembl release 116, antisense: Gm74035.
0 curated, 1 inferred Reactome pathways for Q3UFY7 in mouse, v97.
Reactome
Human NT5C3B by 3 of 3 votes; rat Nt5c3b by 3 of 3 votes. RGD is not answering, so the ortholog list could not be shown. Try again later. Reference bdd2f7d4-2b9.
Alliance · NCBI · Ensembl Compara
300 residues, reviewed Q3UFY7; 11 entries from the member databases this page shows along the chain; mean pLDDT 92.75; no experimental structure at PDBe.
UniProt · InterPro · AlphaFold DB · PDBe
21 RefSeq and 44 Ensembl transcripts on GRCm39; RefSeq Select NM_001102650.2; Ensembl canonical ENSMUST00000092688.13.
NCBI Datasets · Ensembl
Placed on GRCm39 (GCF_000001635.27). MANE Select: not available for mouse. The reference here is the RefSeq Select transcript and the Ensembl canonical transcript, which need not be the same model.
RefSeq 21 transcripts
coding sequence, tall
untranslated region, thin
non-coding exon
intron, fixed width
Genomic strand: minus. Drawn 5' to 3', so exon 1 sits at the left here and at the highest coordinate on the chromosome.
NM_001102650.2NM_001102650.2RefSeq Select
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
NCBI Datasets, RefSeq transcripts · NCBI Datasets 18.38.0; GCF_000001635.27-RS_2024_02 · read · NCBI Gene 68106Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
Ensembl 44 transcripts
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
Lengths are spliced lengths, as each source states them. Exon ranks follow the strand: on a minus-strand gene exon 1 has the highest genomic coordinate. Reference assembly for mouse: GRCm39.
XM_036156927.1XM_036156927.1
XM_030246260.2XM_030246260.2
XM_006534046.5XM_006534046.5
NM_026561.5NM_026561.5
XM_030246258.1XM_030246258.1
XM_036156928.1XM_036156928.1
XM_030246261.2XM_030246261.2
NM_001362966.1NM_001362966.1
XM_036156929.1XM_036156929.1
XM_017314725.1XM_017314725.1
XM_030246259.1XM_030246259.1
NM_001362967.1NM_001362967.1
XM_017314723.1XM_017314723.1
XM_017314727.2XM_017314727.2
XM_036156926.1XM_036156926.1
XM_017314724.3XM_017314724.3
XM_017314726.1XM_017314726.1
XM_036156925.1XM_036156925.1
XM_017314728.1XM_017314728.1
NM_001362968.1NM_001362968.1
scale
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
