Reading the record for Mib2 from NCBI Gene and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 115 seconds for it, and its scripts then bring in the page, or a line saying what did not arrive.
The full name, the identifiers, the location and the notes from the sources arrive with the record. Nothing is filled in ahead of it.
Order door
The door to order for Mib2 opens with the record, which decides which product it carries. The order page itself is open now.
HGNC and MANE Select: not available for rat. HGNC names human genes; RGD is the authority here, and MANE Select is defined for human transcripts only.
Location
Cytogenetic band 5q36NCBI: 5:171,526,037-171,548,070 on the minus strand, GRCr8 (GCF_036323735.1), sequence NC_086023.1, annotation GCF_036323735.1-RS_2026_08 of 2026-08-17Ensembl: 5:171,526,037-171,541,910 on the minus strand, GRCr8 (GCA_036323735.1), release 116Coordinates are one-based with both ends included, as each source reports them.
Gene identity, from NCBI Datasets · NCBI Datasets 18.38.0 · read · RGD:1359469Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
Silence this gene
The order page opens with Mib2 and rat filled in. The sequences are designed against the transcripts below; the price is on that page.
Predicted to enable ubiquitin protein ligase activity.
NCBI Gene summary
Ready in a moment
Reading NCBI Gene and UniProt.Still reading. A first read of a gene can take a while; this page waits up to 30 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
02 / Transcripts and isoforms
The RNA a design targets
50 RefSeq and 2 Ensembl transcripts on GRCr8; RefSeq Select NM_001005901.2; Ensembl canonical ENSRNOT00000100849.2.
NCBI Datasets · Ensembl
Ready in a moment
Reading NCBI Datasets and Ensembl.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
03 / Expression by tissue
Where Mib2 is expressed
Expression by tissue: not available for rat. GTEx holds human tissues only, and the Human Protein Atlas is keyed on human genes.
Ready in a moment
Reading GTEx and the Human Protein Atlas.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.
04 / Protein
The protein Mib2 encodes
Reading UniProt, InterPro, AlphaFold DB and PDBe.Still reading. A first read of a gene can take a while; this page waits up to 80 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
05 / Interactions
Proteins STRING associates with Mib2
Reading STRING.Still reading. A first read of a gene can take a while; this page waits up to 50 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
06 / Pathways
Where Mib2 acts, as Reactome curates it
0 curated, 4 inferred Reactome pathways for Q68LP1 in rat, v97.
Reactome
Ready in a moment
07 / Disease associations
Diseases linked to Mib2
Disease associations: not available for rat. Open Targets and ClinGen curate human genes only. The human ortholog's page carries the associations.
Ready in a moment
Not available
Disease associations: not available for rat. Open Targets and ClinGen curate human genes only. The human ortholog's page carries the associations.
08 / Variants
Classified variants of Mib2
Clinical variants: not available for rat. ClinVar holds human variants only.
Ready in a moment
Not available
Clinical variants: not available for rat. ClinVar holds human variants only.
09 / Constraint
How much variation Mib2 tolerates
Constraint: not available for rat. gnomAD and Open Targets carry constraint for human genes only.
Ready in a moment
Not available
Constraint: not available for rat. gnomAD and Open Targets carry constraint for human genes only.
10 / Orthologs
The same gene in mouse, rat and human
Human MIB2 by 3 of 3 votes; mouse Mib2 by 3 of 3 votes. RGD is not answering, so the ortholog list could not be shown. Try again later. Reference 39aeaa5b-66b.
Alliance · NCBI · Ensembl Compara
Ready in a moment
11 /MicroRNAs
MicroRNAs hosted within Mib2
No annotated microRNA lies within Mib2 in Ensembl release 116, on GRCr8.
Ensembl
Ready in a moment
12 / Long non-coding RNAs
Long non-coding RNAs at the Mib2 locus
No annotated long non-coding RNA overlaps Mib2 in Ensembl release 116, on GRCr8.
Ensembl
Ready in a moment
13 / Literature
Papers that mention Mib2
Reading Europe PMC.Still reading. A first read of a gene can take a while; this page waits up to 55 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.
Ready in a moment
14 / Silencing this gene
From Mib2 to a sequence that silences it
The AUMsilence™ platform designs the sequences against the rat transcripts on this page. Six decisions are yours before it does. What is written under each is AUM's guidance; the timings and the concentrations are in the usage guide below.
01
Choose the region
A knockdown oligonucleotide can sit in the 5' untranslated region, the coding sequence or the 3' untranslated region, and all three are used. The coding sequence and the 3' untranslated region are the usual first choices for an RNase H design; the 5' end near the start codon suits a steric block. The map above shows where each region sits on the isoforms it draws.
02
Cover the isoforms you mean
An exon every isoform carries silences the whole gene; an exon only some isoforms carry silences those and spares the rest. Decide which you want before a sequence is chosen, and check the reference transcript (MANE Select in human; RefSeq Select and Ensembl canonical in mouse and rat) is the one your cells express.
03
Think across species early
The orthologs panel says whether mouse and rat carry the same gene. Whether one oligonucleotide can serve two species is a sequence question, settled at design by matching the candidate against each transcript, not by the protein identity shown there.
04
Check expression in your model
A transcript that is not expressed in your cells cannot show knockdown. Confirm the gene is expressed in the cell type and condition you will use, from your own data or a reference atlas, before the order. The expression panel above gives GTEx's median per tissue for a human gene; for mouse and rat it says that no atlas is on this page yet.
05
Run the controls
A scramble control of the same chemistry, a positive control against a gene known to knock down in your cells, untreated cells, and a mock condition where a transfection reagent is used. Read knockdown at the RNA level first, then at the protein; the usage guide gives the timing and the concentrations to start from.
06
Pick the product
AUMsilence sdASO needs no transfection reagent and works in the cells that resist one. AUMsilence toASO is the transfection-optimised version of the same design, and AUMsiRNA™ is the siRNA route. The selection guide compares them.
For research use only. Not for use in diagnostic or therapeutic procedures.
Gene summary
Predicted to enable ubiquitin protein ligase activity. Involved in regulation of postsynaptic neurotransmitter receptor internalization and regulation protein catabolic process at postsynapse. Is active in glutamatergic synapse and postsynapse. Orthologous to human MIB2 (MIB E3 ubiquitin protein ligase 2).
Provided by Alliance of Genome Resources, Jul 2025, through NCBI Gene. NCBI disclaimer
E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors
NCBI Gene summary · NCBI Gene annotation RS_2026_08 · read · NCBI Gene 474147Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
UniProtKB function · 2026_03 · read · UniProt Q68LP1UniProt data are available under the Creative Commons Attribution 4.0 licence.
Placed on GRCr8 (GCF_036323735.1). MANE Select: not available for rat. The reference here is the RefSeq Select transcript and the Ensembl canonical transcript, which need not be the same model.
RefSeq 50 transcripts
coding sequence, tall
untranslated region, thin
non-coding exon
intron, fixed width
Genomic strand: minus. Drawn 5' to 3', so exon 1 sits at the left here and at the highest coordinate on the chromosome.
NM_001005901.2NM_001005901.2RefSeq Select
XM_087618615.1XM_087618615.1
XM_087618649.1XM_087618649.1
XM_087618651.1XM_087618651.1
XM_087618650.1XM_087618650.1
XM_087618632.1XM_087618632.1
XM_087618614.1XM_087618614.1
XM_087618618.1XM_087618618.1
XM_063288166.2XM_063288166.2
XM_017593555.4XM_017593555.4
XM_087618619.1XM_087618619.1
XM_087618636.1XM_087618636.1
XM_087618634.1XM_087618634.1
XM_087618622.1XM_087618622.1
XM_087618629.1XM_087618629.1
XM_087618644.1XM_087618644.1
XM_087618637.1XM_087618637.1
XM_087618652.1XM_087618652.1
XM_087618645.1XM_087618645.1
XM_087618656.1XM_087618656.1
XM_087618616.1XM_087618616.1
XM_087618657.1XM_087618657.1
XM_087618621.1XM_087618621.1
XM_087618617.1XM_087618617.1
XM_017593553.4XM_017593553.4
XM_087618623.1XM_087618623.1
XM_087618620.1XM_087618620.1
XM_087618630.1XM_087618630.1
XM_087618627.1XM_087618627.1
XM_087618625.1XM_087618625.1
XM_087618633.1XM_087618633.1
XM_087618628.1XM_087618628.1
XM_087618658.1XM_087618658.1
XM_087618640.1XM_087618640.1
XM_087618624.1XM_087618624.1
XM_017593554.4XM_017593554.4
XM_087618631.1XM_087618631.1
XM_087618638.1XM_087618638.1
XM_087618635.1XM_087618635.1
XM_087618641.1XM_087618641.1
XM_087618647.1XM_087618647.1
XM_087618626.1XM_087618626.1
XM_087618646.1XM_087618646.1
XM_087618654.1XM_087618654.1
XM_087618639.1XM_087618639.1
XM_087618653.1XM_087618653.1
XM_087618643.1XM_087618643.1
XM_087618648.1XM_087618648.1
XM_087618655.1XM_087618655.1
XM_087618659.1XM_087618659.1
scale
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
Not listed, because the source places them on another assembly only: XM_087550347.1 (not placed on GRCr8); XM_087550348.1 (not placed on GRCr8); XM_087550349.1 (not placed on GRCr8); XM_087550351.1 (not placed on GRCr8); XM_087550352.1 (not placed on GRCr8); XM_087550353.1 (not placed on GRCr8); XM_087550354.1 (not placed on GRCr8); XM_087550355.1 (not placed on GRCr8); XM_087550356.1 (not placed on GRCr8); XM_087550357.1 (not placed on GRCr8); XM_087550358.1 (not placed on GRCr8); XM_087550359.1 (not placed on GRCr8); XM_087550360.1 (not placed on GRCr8); XM_087550362.1 (not placed on GRCr8); XM_087550363.1 (not placed on GRCr8); XM_087550364.1 (not placed on GRCr8); XM_087550365.1 (not placed on GRCr8); XM_087550366.1 (not placed on GRCr8); XM_087550367.1 (not placed on GRCr8); XM_087550368.1 (not placed on GRCr8); XM_087550369.1 (not placed on GRCr8); XM_087550370.1 (not placed on GRCr8); XM_087550371.1 (not placed on GRCr8); XM_087550373.1 (not placed on GRCr8); XM_087550374.1 (not placed on GRCr8); XM_087550375.1 (not placed on GRCr8); XM_087550376.1 (not placed on GRCr8); XM_087550377.1 (not placed on GRCr8); XM_087550378.1 (not placed on GRCr8); XM_087550379.1 (not placed on GRCr8); XM_087550380.1 (not placed on GRCr8); XM_087550381.1 (not placed on GRCr8); XM_087550382.1 (not placed on GRCr8); XM_087550383.1 (not placed on GRCr8); XM_087550384.1 (not placed on GRCr8); XM_087550385.1 (not placed on GRCr8); XM_087550386.1 (not placed on GRCr8); XM_087550387.1 (not placed on GRCr8); XM_087550388.1 (not placed on GRCr8); XM_087550389.1 (not placed on GRCr8); XM_087550390.1 (not placed on GRCr8); XM_087550391.1 (not placed on GRCr8); XM_087550392.1 (not placed on GRCr8); XM_087550393.1 (not placed on GRCr8); XM_087550394.1 (not placed on GRCr8); XM_087550395.1 (not placed on GRCr8); XM_087550396.1 (not placed on GRCr8); XM_087550397.1 (not placed on GRCr8); XM_087550399.1 (not placed on GRCr8).
NCBI Datasets, RefSeq transcripts · NCBI Datasets 18.38.0; GCF_036323735.1-RS_2026_08 · read · NCBI Gene 474147Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
Ensembl 2 transcripts
coding sequence, tall
untranslated region, thin
non-coding exon
intron, fixed width
Genomic strand: minus. Drawn 5' to 3', so exon 1 sits at the left here and at the highest coordinate on the chromosome.
Drawn 5' to 3' from each transcript's exons as placed on the reference assembly; exon 1 is the 5' exon on the transcript's own strand. Exon blocks are to scale with each other; introns are drawn at one fixed width whatever their length, so the map is not to scale along the chromosome. Numbers are exon ranks along the strand; a rank is omitted where the exon is too narrow to carry it.
A window on one transcript
One pixel of the map above stands for several bases, and a block too short to see is drawn wider than its own scale, so the map chooses a region and the sequence here chooses the window. Click an exon on a row of the map, or drag across a row; then set the exact start and end below.
These controls are ready in a moment.
No transcript is chosen.
Once a window is chosen this panel shows its length, its G and C count as a percentage of that length, the letters it is made of, the exons it falls in, whether it crosses a junction, and its antisense strand.
Lengths are spliced lengths, as each source states them. Exon ranks follow the strand: on a minus-strand gene exon 1 has the highest genomic coordinate. Reference assembly for rat: GRCr8.
Expression by tissue: not available for rat. GTEx holds human tissues only, and the Human Protein Atlas is keyed on human genes.
Rat tissue expression, from Bgee and Expression Atlas, joins this page in a later phase. Nothing from the human ortholog is shown in its place; its own page is a step away through the species switch above.
The 25 highest-scoring STRING partners at or above a combined score of 0.4, of up to 25 asked for; Uba52, Cyld, Traf2 lead.
STRING v12.0
Europe PMC did not answer in time, so the literature search could not be shown. Try again in a minute. Reference bc7af25c-672.
971 residues, reviewed Q68LP1; 28 entries from the member databases this page shows along the chain; mean pLDDT 83.31; no experimental structure at PDBe.
