MicroRNA Mouse Mus musculus

mmu-miR-200b-5p

The 5p arm of the hairpin mmu-mir-200b (MI0000243); the other arm is mmu-miR-200b-3p.

Sequence of mmu-miR-200b-5p: CAUCUUACUG GGCAGCAUUG GA

22 nt, as miRBase release 23 writes it, 5' to 3'; unchanged in miRBase release 23.

Rat: no vote names an ortholog of Mir200b: the Alliance, NCBI and Ensembl Compara answered and named none; RGD did not answer (upstream error, reference 8bc5277e-f01).

HumanMouseRat

Reading the ortholog votes for human and rat.

Gene placement: NCBI: 4:156,140,138-156,140,207 on the minus strand, GRCm39 (GCF_000001635.27), sequence NC_000070.7, annotation GCF_000001635.27-RS_2024_02Ensembl: 4:156,140,138-156,140,207 on the minus strand, GRCm39 (GCA_000001635.9), Ensembl release 116
Host gene: Lies within Gm13648, on the same strand; Lies within Gm69145, on the opposite strand; Lies within Ttll10, on the same strand (Ensembl release 116, GRCm39).
The gene is also known as: Mirn200b, mir-200b (names of the gene Mir200b, as NCBI Datasets 18.38.0 lists them)

miRBase placement: The mature: 4:156,140,178-156,140,199 on the minus strand, GRCm39 (GCA_000001635.9)The hairpin mmu-mir-200b: 4:156,140,138-156,140,207 on the minus strand, GRCm39 (GCA_000001635.9)Coordinates are one-based with both ends included, as each source reports them.

MicroRNA identity, from miRBase · miRBase v23 (2026-07-29), GRCm39 · read 2026-10-06 ET · miRBase MIMAT0004545miRBase release 23; Kozomara A, Birgaoanu M, Griffiths-Jones S. Nucleic Acids Research (2019).

Inhibit or mimic this microRNA

The order page opens with mmu-miR-200b-5p and mouse filled in; the price is on that page.

Order for mmu-miR-200b-5p

AUMantagomir sdASO (inhibitor) or AUMmimic sdASO (mimic)

Bring your own sequence The hairpin's gene page

For research use only. Not for use in diagnostic or therapeutic procedures.

Sequence and arms
The gene and its locus
In other species
Cross-references
Pathways
Targets
Literature
AUM BioTech's studies
Inhibit or mimic this microRNA

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01 Sequence and arms

The mature, its arm and its hairpin

mmu-miR-200b-5p is 22 nt, the 5p arm of the 70 nt hairpin mmu-mir-200b; the other arm is mmu-miR-200b-3p. Its sequence did not change in miRBase release 23.

miRBase

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What this tells you

miRBase's files name this mature, its accession, its sequence and the hairpin it derives from, and the GFF3 places both on the genome. Of the hairpins the GFF3's header says: “Note, these sequences do not represent the full primary transcript, rather a predicted stem-loop portion that includes the precursor miRNA.”

A sentence from RefSeq's summary on the gene, as NCBI Gene carries it: “The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products.” [RefSeq, Sep 2009].

The arm suffix is miRBase's naming. The README of release 23 says: “20514 mature sequences have had their names clarified, usually upon the addition of a 2nd mature product from the same locus. For example, hsa-miR-107 is renamed to hsa-miR-107-3p on the addition of hsa-miR-107-5p to the database.” The convention that mir names a hairpin and miR a mature is the annotation guidelines paper the README cites: Ambros V, Bartel B, Bartel DP, Burge CB, Carrington JC, Chen X, Dreyfuss G, Eddy SR, Griffiths-Jones S, Marshall M, Matzke M, Ruvkun G, Tuschl T. A uniform system for microRNA annotation. RNA 2003 9(3):277-279. PubMed 12592000, DOI 10.1261/rna.2183803. miRBase itself is cited as the credit line carries it: Kozomara A, Birgaoanu M, Griffiths-Jones S. miRBase: from microRNA sequences to function. Nucleic Acids Res 2019 47(D1):D155-D162. PubMed 30423142, DOI 10.1093/nar/gky1141.

The seed drawn on the mature is a cited definition, not a measurement. MirGeneDB's information page (read 2026-09-08) says: “Nucleotide positions 2 through 8 of the mature sequence (the "seed") are strongly conserved through evolution, as are positions 13-16 (the 3' complementary region) (13,20,21).” TargetScan's FAQ defines its site types by the same positions counted two ways: “7mer-m8: An exact match to positions 2-8 of the mature miRNA (the seed + position 8)” and “7mer-1A: An exact match to positions 2-7 of the mature miRNA” followed by an A. So TargetScan's seed proper is positions 2 to 7, and the span this page shades, 2 to 8, is MirGeneDB's.

The two arms are different microRNAs, so an antagomir or a mimic is made against one of them; the other arm has its own page, and the order door of this page carries this arm's name.

The mature sequence

Positions 1 to 22 of mmu-miR-200b-5p, 5' to 3', as MirGeneDB's definition counts them. The shaded positions 2 to 8 are the seed region, nucleotides 2 to 8, as MirGeneDB's information page defines it (read 2026-09-08); nothing else on the sequence is marked.

The records of mmu-miR-200b-5p as text
Mature name	mmu-miR-200b-5p
Accession	MIMAT0004545
Sequence, 5' to 3'	CAUCUUACUGGGCAGCAUUGGA
Length	22 nt, as miRBase release 23 writes it
Seed, positions 2 to 8	AUCUUAC
Changed in this release	no: the diff of miRBase release 23 carries no SEQUENCE line for MIMAT0004545
Placement	4:156,140,178-156,140,199 on the minus strand, GRCm39 (GCA_000001635.9)

The hairpin mmu-mir-200b MI0000243 70 nt

The hairpin mmu-mir-200b drawn as a line, 5' to 3': each mature at the positions the records give it, the bases between two matures labelled the loop, and the bases before and after them the flanks. No fold is drawn: the shape of the hairpin is not in any file this page reads.

The segments of mmu-mir-200b as text
Segment	Positions	Length	Bases, 5' to 3'
5' flank

The hairpin and the matures are written as RNA, with U, as miRBase's files write them; the genomic and RefSeq records of the same span are written as DNA, with T. miRBase's entry page for MIMAT0004545 carries an Evidence line that no downloadable file carries, so it is not on this page; read it there.

Mature and hairpin sequences and spans, from miRBase · miRBase v23 (2026-07-29), GRCm39 · read 2026-10-06 ET · miRBase MIMAT0004545miRBase release 23; Kozomara A, Birgaoanu M, Griffiths-Jones S. Nucleic Acids Research (2019).
Seed definition, from MirGeneDB · MirGeneDB information page, read 2026-09-08 · MirGeneDB information

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02 The gene and its locus

The gene of mmu-mir-200b and where it sits

Mir200b (MGI:2676875), 4 E2, within Gm13648 on the same strand and within Gm69145 on the opposite strand and within Ttll10 on the same strand. RefSeq NR_029587.1, 70 nt.

NCBI Gene · MGI · Ensembl

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What this tells you

The hairpin is a gene to the nomenclature authorities and to NCBI Gene and Ensembl: HGNC for human, MGI for mouse and RGD for rat name it, NCBI places it on the reference annotation and RefSeq holds its stem-loop record, and Ensembl places it and says what other gene its span lies inside. Each placement is on the assembly its source names, and the card never converts one into another.

A sentence from RefSeq's summary on the gene, as NCBI Gene carries it: “miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding.” [RefSeq, Sep 2009].

The gene page holds the locus's own records: the transcripts, the variants and the disease associations are the gene's, and this card links to it rather than repeating them as if they were this arm's.

The gene Mir200b of the hairpin mmu-mir-200b

Symbol

Mir200b microRNA 200b

Type

non-coding RNA ncRNA

MGI

MGI:2676875

NCBI Gene

387243

Ensembl

ENSMUSG00000065549.5

RefSeq records

NR_029587.1 70 nt, the length of the hairpin in miRBase release 23 non-coding (NON_CODING) Ensembl ENSMUST00000083615.5
Annotation GCF_000001635.27-RS_2024_02.

Cytogenetic band

4 E2

Also known as

Mirn200b, mir-200b

RefSeq's summary on the gene Mir200b, as NCBI Gene carries it, quoted as written.

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.
[provided by RefSeq, Sep 2009]

Placements, each on the assembly its source names

miRBase, the hairpin mmu-mir-200b: 4:156,140,138-156,140,207 on the minus strand, GRCm39 (GCA_000001635.9)
NCBI, the gene: 4:156,140,138-156,140,207 on the minus strand, GRCm39 (GCF_000001635.27), sequence NC_000070.7, annotation GCF_000001635.27-RS_2024_02
Ensembl, the gene: 4:156,140,138-156,140,207 on the minus strand, GRCm39 (GCA_000001635.9), Ensembl release 116

Coordinates are one-based with both ends included, as each source reports them.

The host gene of Mir200b, from Ensembl's overlap

Gm13648 ENSMUSG00000086549.3lncRNA

predicted gene 13648 (Ensembl credits MGI Symbol, Acc:MGI:3652033)

4:156,137,172-156,144,278 on the minus strand, GRCm39 (GCA_000001635.9); Mir200b lies within the span of Gm13648; sense, on the same strand as Gm13648.

Gm69145 ENSMUSG00000132474.1lncRNA

predicted gene, 69145 (Ensembl credits MGI Symbol, Acc:MGI:7821107)

4:156,138,983-156,141,455 on the plus strand, GRCm39 (GCA_000001635.9); Mir200b lies within the span of Gm69145; antisense, on the opposite strand to Gm69145.

Ttll10 ENSMUSG00000029074.16protein_coding

tubulin tyrosine ligase-like family, member 10 (Ensembl credits MGI Symbol, Acc:MGI:1921855)

4:156,119,294-156,143,871 on the minus strand, GRCm39 (GCA_000001635.9); Mir200b lies within the span of Ttll10; sense, on the same strand as Ttll10.

The Mir200b gene pageThe locus's own records, the transcripts, the variants and the disease associations, are the gene's and are on that page.

Gene record and placements, from MGI, NCBI Gene and Ensembl · miRBase v23 (2026-07-29); NCBI Datasets 18.38.0 · read 2026-10-06 ET · NCBI Gene 387243Data from NCBI, provided as is; NCBI's policies and disclaimers apply.
Hairpin and mature placements, from miRBase · miRBase v23 (2026-07-29), GRCm39 · read 2026-10-06 ET · miRBase MI0000243miRBase release 23; Kozomara A, Birgaoanu M, Griffiths-Jones S. Nucleic Acids Research (2019).
Host gene of Mir200b, from Ensembl's overlap · Ensembl 116 · read 2026-10-06 ET · Ensembl ENSMUSG00000086549Ensembl release 116, GRCm39 (GCA_000001635.9)
RefSeq records of Mir200b, from NCBI Datasets · NCBI Datasets 18.38.0; GCF_000001635.27-RS_2024_02 · read 2026-10-06 ET · NR_029587.1NCBI Datasets 18.38.0; annotation GCF_000001635.27-RS_2024_02 (public domain)

Reading NCBI Gene, the nomenclature authority and Ensembl.Still reading. A first read of a microRNA can take a while; this page waits up to 90 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.

03 In other species

The same arm in mouse, rat and human

Human hsa-miR-200b-5p (MIR200B, by the Alliance and Ensembl Compara); rat: no vote names an ortholog (the Alliance, NCBI and Ensembl Compara answered and named none; RGD did not answer (upstream error, reference 8bc5277e-f01)). The same-arm sequences share 22 of 22 compared positions with human.

Alliance · NCBI · Ensembl Compara · miRBase

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04 Cross-references

The sequence at RNAcentral and its member databases

Listed by 7 member databases at RNAcentral (URS000012A1DD) for mouse. RNAcentral's copy of the sequence is miRBase release 23's.

RNAcentral

Reading RNAcentral.Still reading. A first read of a microRNA can take a while; this page waits up to 60 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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05 Pathways

Where Reactome places mmu-miR-200b-5p

Reactome v97 maps no pathway to mmu-miR-200b-5p (MIMAT0004545) or to mmu-mir-200b (MI0000243) in mouse.

Reactome

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06 Targets

Where the target databases are

Predicted and validated targets are held by databases whose terms do not grant display on a company's website; 6 doors open them for mmu-miR-200b-5p.

Reading RNAcentral and the ortholog votes, for the doors.Still reading. A first read of a microRNA can take a while; this page waits up to 120 seconds for it, and its scripts then bring in this line, or a line saying what did not arrive.

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07 Literature

Papers that mention mmu-miR-200b-5p or its base name miR-200b

10,621 PubMed-indexed papers match the query for mmu-miR-200b-5p or its base name miR-200b at Europe PMC, 25 a page.

Europe PMC

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08 AUM BioTech's studies on this microRNA

AUM BioTech's published work on mmu-miR-200b-5p

No checked AUM BioTech record names mmu-miR-200b-5p in mouse.

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09 Inhibit or mimic this microRNA

Targeting mmu-miR-200b-5p

The order page opens with mmu-miR-200b-5p and mouse filled in, with AUMantagomir sdASO and AUMmimic sdASO side by side and the price on that page. Five decisions are yours before it does. What is written under each is AUM's guidance; the timings and the concentrations are in the usage guide below.

01
Choose the arm
The mature named on this page is one arm of its hairpin; the other arm, mmu-miR-200b-3p, is a different microRNA with its own page. An inhibitor or a mimic is made against one arm, and the order door of this page carries mmu-miR-200b-5p.
02
Inhibit or replace
An AUMantagomir sdASO inhibits the mature microRNA; an AUMmimic sdASO supplies one. Both are self-delivering. AUMantagomir toASO is the transfection-optimised version of AUMantagomir sdASO, a lower-cost option that needs a transfection reagent.
03
Check the species
The sequence is mouse's, as miRBase release 23 writes it. Where the orthologs card shows the same arm in another species with another sequence, a design for one species is not a design for the other; where the bases are the same, that is a sequence fact the card states, not a claim about the two.
04
Run the controls
A scramble control of the same chemistry, the positive control the order page offers for the product, untreated cells, and a mock condition wherever a transfection reagent is used. The order page lists the controls beside the product.
05
Read the result at the target level
Inhibiting or supplying a microRNA is read in what it acts on, not in the microRNA alone: the transcripts and the proteins your own experiment names as its targets. The usage guide gives the timing and the concentrations to start from; nothing on this page names a target.

Order for mmu-miR-200b-5p Bring your own sequence The usage guide

AUMantagomir sdASO (inhibitor) or AUMmimic sdASO (mimic)

For research use only. Not for use in diagnostic or therapeutic procedures.

mmu-miR-200b-5p

The mature, its arm and its hairpin

The mature sequence

The hairpin mmu-mir-200b MI0000243 70 nt

The gene of mmu-mir-200b and where it sits

The gene Mir200b of the hairpin mmu-mir-200b

Placements, each on the assembly its source names

The host gene of Mir200b, from Ensembl's overlap

The same arm in mouse, rat and human

The sequence at RNAcentral and its member databases

Where Reactome places mmu-miR-200b-5p

Where the target databases are

Papers that mention mmu-miR-200b-5p or its base name miR-200b

AUM BioTech's published work on mmu-miR-200b-5p

Targeting mmu-miR-200b-5p

Choose the arm

Inhibit or replace

Check the species

Run the controls

Read the result at the target level

The mature sequence

The hairpin mmu-mir-200b MI0000243 70 nt

The gene Mir200b of the hairpin mmu-mir-200b

Placements, each on the assembly its source names

The host gene of Mir200b, from Ensembl's overlap