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Configure your RNA-silencing products

Choose a product family, name the target or paste your sequence, then set purification and yield.

Self-delivering antisense, taken up by cells without a transfection reagent: AUMsilence, AUMantagomir, AUMlnc and AUMmimic sdASOs, designed to the targets you name or made from your own sequences.

Quotation request.

01 How do you want to start?

Name the targets and AUM designs, screens and makes the sequences.

02 Product
03 Species and microRNA

The mature name, without the species prefix.

04 Purification

Reverse-phase cartridge (RPC) or high-performance liquid chromatography (HPLC).

05 Delivered yield

10 nmol: enough for 2 wells of a 24-well plate at 10 μM, one treatment each.

How much to order?
Medium in the well

A titration at 5 μM, 10 μM and 20 μM in triplicate is 9 wells. The wells are spread over the series.

9 wells: 3 at each of 5 μM, 10 μM and 20 μM.

The cells

1 dose: 3 days is within one re-dosing interval.

You have 10 nmol selected. In 500 μL that is 2 treatments at 10 μM.

For 9 wells over 5 μM, 10 μM and 20 μM in one dose the experiment needs 52.5 nmol, or 60.38 nmol with a 15% margin. The 100 nmol yield covers it with 7 treatments left.

Also consider: 50 nmol, the yield below, covers 8 wells like these, not the whole experiment.

You have 10 nmol selected. In 500 μL that is 2 treatments at 10 μM. For 9 wells over 5 μM, 10 μM and 20 μM in one dose the experiment needs 52.5 nmol, or 60.38 nmol with a 15% margin. The 100 nmol yield covers it with 7 treatments left.

How much to order

One sequence per target.

06 Controls

A scramble control is recommended with every order; a labeled control shows uptake in your cells before the first experiment.

Positive control

10 nmol, RPC, one tube per control chosen. A microRNA absent from mammalian cells, so the effect is the delivered mimic alone.

Labeled scramble control

10 nmol, HPLC, $799. Fluorescent, to see uptake.

For research use only. Not for use in diagnostic or therapeutic procedures.