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Configure your RNA-silencing products

Choose a product family, name the target or paste your sequence, then set purification and yield.

Transfection-optimized antisense, delivered with your own transfection reagent: AUMsilence, AUMantagomir and AUMlnc toASOs, singly or as a pool of four sequences against one target.

Quotation request.

01 How do you want to start?

Name the targets and AUM designs, screens and makes the sequences.

02 Product
03 Species and target gene

The official symbol, an alias or a previous symbol.

04 Format
05 Purification

Reverse-phase cartridge (RPC) or high-performance liquid chromatography (HPLC).

06 Delivered yield

2 nmol: enough for 66 wells of a 24-well plate at 50 nM, one transfection each.

How much to order?
Volume in the well

The complete medium plus the two dilutions the transfection protocol adds to it.

The wells are spread over 50 nM and 100 nM, the working range.

6 wells: 3 at each of 50 nM and 100 nM.

The cells

1 dose: 3 days is within one re-dosing interval.

You have 2 nmol selected. In 600 μL, the complete medium plus the two dilutions, that is 66 transfections at 50 nM.

For 6 wells over 50 nM and 100 nM in one dose the experiment needs 0.27 nmol, or 0.31 nmol with a 15% margin. The 2 nmol yield you have selected covers it with 56 transfections left.

Also consider: 5 nmol, the yield above, covers 111 wells like these, the whole experiment included.

You have 2 nmol selected. In 600 μL, the complete medium plus the two dilutions, that is 66 transfections at 50 nM. For 6 wells over 50 nM and 100 nM in one dose the experiment needs 0.27 nmol, or 0.31 nmol with a 15% margin. The 2 nmol yield you have selected covers it with 56 transfections left.

How much to order

07 Number per target
08 Controls

A scramble control is recommended with every order; a labeled control shows uptake in your cells before the first experiment.

Positive control

2 nmol, RPC, one tube per control chosen. A housekeeping gene, to confirm uptake and knockdown.

Labeled scramble control

10 nmol, HPLC, $799. Fluorescent, to see uptake.

For research use only. Not for use in diagnostic or therapeutic procedures.