Pathway Human Homo sapiens

RNA Polymerase III Transcription

R-HSA-74158 in Reactome release 97: under Gene expression (Transcription), with 41 genes placed in it by the mapping files and 4 child pathways in the hierarchy.

The same number in the other species

Reactome writes the mouse and rat events it infers from a human pathway under the human number with the species token. A door opens only where that species' own list in this release holds the id: R-MMU-74158 (mouse), R-RNO-74158 (rat). Whether the event was inferred from this one is what the record says.

The record
The genes
Parents and children

Scripts are off

This page reads the pathway's record from its source while you read the rest; with scripts off the card stays at its loading line. Reload with scripts on to see it, or open the source's own page from the link in the card.

01The record

Reactome's own record of this pathway

What this tells you

The pathway list, the hierarchy and the genes on this page are read from the files Reactome publishes for Reactome release 97, built into this site on 2026-09-09: the pathway list, the hierarchy relationship file and the two gene mapping files. The record card is the one live read, Reactome's own record of this pathway.

Reactome's download page describes the mapping files: Mapping files link the source database identifier to the lowest level pathway diagram or subset of the pathway, all levels of the pathway hierarchy or database identifier to all reactions. [R13] The gene list here is the all-levels mapping of NCBI Gene ids, filled from the all-levels mapping of Ensembl ids where the NCBI file has no row for a gene; each row names which file it came from by the shape of its source id. Each row carries the evidence codes the file writes for it, as written and unranked, and both where the file writes both; no page of Reactome's documentation the survey read defines the codes, so this page does not expand them. A gene id the identity files do not name is kept as the source's own id with no door rather than turned into a symbol.

On citing what a search finds, Reactome says: It should be remembered that while we strive to contain the most current and accurate data, Reactome should not be used in citations where other primary sources of information are available. [R12] The record card prints the literature Reactome attaches to a curated pathway for that reason. The data are public domain: All data in the Reactome database and files derived from that data are licensed under the Creative Commons Public Domain Dedication (CC0). User may copy, modify, and distribute these data, even for commercial purposes, without asking for permission. Attribution is encouraged but not required. [R10] The pathway illustrations are licensed apart, under CC BY 4.0, and none is shown here.

A gene on this list is one Reactome places in this pathway in this release, and that is all the row says: it does not say the gene is expressed in a tissue or associated with a disease, which are the other two explorers' questions, read from other sources. Reactome's papers of record are [R01] [R02].

[R01] Ragueneau E, Gong C, Sinquin P, Sevilla C, Beavers D, Grentner A, et al. (2026). The Reactome Knowledgebase 2026. Nucleic Acids Research 54:D673-D681. PMID 41251150, doi 10.1093/nar/gkaf1223.
[R02] Milacic M, Beavers D, Conley P, Gong C, Gillespie M, Griss J, et al. (2024). The Reactome Pathway Knowledgebase 2024. Nucleic Acids Research 52:D672-D678. PMID 37941124, doi 10.1093/nar/gkad1025.
[R10] Reactome, reactome.org. License Agreement. https://reactome.org/license, read 2026-09-09.
[R12] Reactome, reactome.org. Citing us. https://reactome.org/cite, read 2026-09-09.
[R13] Reactome, reactome.org. Download. https://reactome.org/download-data, read 2026-09-09.

Stable id: R-HSA-74158.4
Class: Pathway
Species: Homo sapiens (human)
Inferred: no: a curated human pathway
Disease pathway: no
Released: 2004-07-06
Last updated: 2021-12-08
Events inside: 4, counted from the record

RNA polymerase III is one of three types of nuclear RNA polymerases present in eucaryotic cells. About 10% of the total transcription in dividing cells can be attributed to its activity. It synthesizes an eclectic collection of catalytic or structural RNA molecules, some of which are involved in protein synthesis, pre-mRNA splicing, tRNA processing, and the control of RNA polymerase II elongation, whereas some others have still unknown functions. Like other RNA polymerases, RNA polymerase III cannot recognize its target promoters directly. Instead it is recruited to specific promoter sequences through the help of transcription factors. There are three basic types of RNA polymerase III promoters, called types 1, 2, and 3(Geiduschek and Kassavetis, 1992). Although in vivo, RNA polymerase III may be recruited to these promoters as part of a large complex (holo RNA polymerase III) containing the polymerase and its initiation factors (Wang et al., 1997), in vitro the reaction can be divided into several steps. First, the promoter elements are recognized by DNA binding factors, which then recruit a factor known as TFIIIB. TFIIIB itself then directly contacts RNA polymerase III. In human cells but not in S. cerevisiae, there are at least two versions of TFIIIB. One contains TBP, Bdp1, and Brf1 (Brf1-TFIIIB), and the other TBP, Bdp1, and Brf2 (Brf2-TFIIIB) (Schramm et al., 2000; Teichmann et al., 2000).

Literature Reactome attaches

A stable complex of a novel transcription factor IIB- related factor, human TFIIIB50, and associated proteins mediate selective transcription by RNA polymerase III of genes with upstream promoter elementsProc Natl Acad Sci U S A, 2000, vol. 97, p. 14200-5PubMed 11121026
Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promotersGenes Dev, 2000, vol. 14, p. 2650-63PubMed 11040218
Identification of an autonomously initiating RNA polymerase III holoenzyme containing a novel factor that is selectively inactivated during protein synthesis inhibitionGenes Dev, 1997, vol. 11, p. 2371-82PubMed 9308965
Transcriptional Regulation1992, p. 247-280

Reactome's record lists 12 orthologous events, R-RNO-74158 (Rattus norvegicus), R-MMU-74158 (Mus musculus) among the species this site serves, and 10 in species this site opens no page for.

Reactome ContentService, this pathway's record · Reactome v97 · read 2026-09-30 ET · Reactome pathway browser"RNA Polymerase III Transcription". Reactome, release 97, https://reactome.org/PathwayBrowser/#/R-HSA-74158 with StableID: R-HSA-74158.4 (accessed 2026-09-30 ET). Reactome version 97 (CC0).

Reading this pathway's record from Reactome (the pathway record).Still reading. A first read of a pathway can take a while; this page waits up to 35 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.

02The genes

Genes Reactome places in this human pathway

The mapping files place 41 genes in this human pathway; showing 1 to 41, in pages of 100, sorted by symbol for reading. The order carries no ranking.

Genes Reactome places in this human pathway, page 1 of 1
Gene	Authority id	Mapping file id	Evidence codes
GeneBDP1	AuthorityHGNC:13652	Mapping file id55814 NCBI file	EvidenceIEA, TAS
GeneBRF1	AuthorityHGNC:11551	Mapping file id2972 NCBI file	EvidenceIEA, TAS
GeneBRF2	AuthorityHGNC:17298	Mapping file id55290 NCBI file	EvidenceIEA, TAS
GeneCRCP	AuthorityHGNC:17888	Mapping file id27297 NCBI file	EvidenceIEA, TAS
GeneGTF3A	AuthorityHGNC:4662	Mapping file id2971 NCBI file	EvidenceIEA, TAS
GeneGTF3C1	AuthorityHGNC:4664	Mapping file id2975 NCBI file	EvidenceIEA, TAS
GeneGTF3C2	AuthorityHGNC:4665	Mapping file id2976 NCBI file	EvidenceIEA, TAS
GeneGTF3C3	AuthorityHGNC:4666	Mapping file id9330 NCBI file	EvidenceIEA, TAS
GeneGTF3C4	AuthorityHGNC:4667	Mapping file id9329 NCBI file	EvidenceIEA, TAS
GeneGTF3C5	AuthorityHGNC:4668	Mapping file id9328 NCBI file	EvidenceIEA, TAS
GeneGTF3C6	AuthorityHGNC:20872	Mapping file id112495 NCBI file	EvidenceIEA, TAS
GeneNFIA	AuthorityHGNC:7784	Mapping file id4774 NCBI file	EvidenceIEA
GeneNFIB	AuthorityHGNC:7785	Mapping file id4781 NCBI file	EvidenceIEA
GeneNFIC	AuthorityHGNC:7786	Mapping file id4782 NCBI file	EvidenceIEA
GeneNFIX	AuthorityHGNC:7788	Mapping file id4784 NCBI file	EvidenceIEA
GenePOLR1C	AuthorityHGNC:20194	Mapping file id9533 NCBI file	EvidenceIEA, TAS
GenePOLR1D	AuthorityHGNC:20422	Mapping file id51082 NCBI file	EvidenceIEA, TAS
GenePOLR2E	AuthorityHGNC:9192	Mapping file id5434 NCBI file	EvidenceIEA, TAS
GenePOLR2F	AuthorityHGNC:9193	Mapping file id5435 NCBI file	EvidenceIEA, TAS
GenePOLR2H	AuthorityHGNC:9195	Mapping file id5437 NCBI file	EvidenceIEA, TAS
GenePOLR2K	AuthorityHGNC:9198	Mapping file id5440 NCBI file	EvidenceIEA, TAS
GenePOLR2L	AuthorityHGNC:9199	Mapping file id5441 NCBI file	EvidenceIEA, TAS
GenePOLR3A	AuthorityHGNC:30074	Mapping file id11128 NCBI file	EvidenceIEA, TAS
GenePOLR3B	AuthorityHGNC:30348	Mapping file id55703 NCBI file	EvidenceIEA, TAS
GenePOLR3C	AuthorityHGNC:30076	Mapping file id10623 NCBI file	EvidenceIEA, TAS
GenePOLR3D	AuthorityHGNC:1080	Mapping file id661 NCBI file	EvidenceIEA, TAS
GenePOLR3E	AuthorityHGNC:30347	Mapping file id55718 NCBI file	EvidenceIEA, TAS
GenePOLR3F	AuthorityHGNC:15763	Mapping file id10621 NCBI file	EvidenceIEA, TAS
GenePOLR3G	AuthorityHGNC:30075	Mapping file id10622 NCBI file	EvidenceIEA, TAS
GenePOLR3GL	AuthorityHGNC:28466	Mapping file id84265 NCBI file	EvidenceIEA, TAS
GenePOLR3H	AuthorityHGNC:30349	Mapping file id171568 NCBI file	EvidenceIEA, TAS
GenePOLR3K	AuthorityHGNC:14121	Mapping file id51728 NCBI file	EvidenceIEA, TAS
GenePOU2F1	AuthorityHGNC:9212	Mapping file id5451 NCBI file	EvidenceIEA, TAS
GeneSNAPC1	AuthorityHGNC:11134	Mapping file id6617 NCBI file	EvidenceIEA, TAS
GeneSNAPC2	AuthorityHGNC:11135	Mapping file id6618 NCBI file	EvidenceIEA, TAS
GeneSNAPC3	AuthorityHGNC:11136	Mapping file id6619 NCBI file	EvidenceIEA, TAS
GeneSNAPC4	AuthorityHGNC:11137	Mapping file id6621 NCBI file	EvidenceIEA, TAS
GeneSNAPC5	AuthorityHGNC:15484	Mapping file id10302 NCBI file	EvidenceIEA, TAS
GeneSSB	AuthorityHGNC:11316	Mapping file id6741 NCBI file	EvidenceIEA
GeneTBP	AuthorityHGNC:11588	Mapping file id6908 NCBI file	EvidenceIEA, TAS
GeneZNF143	AuthorityHGNC:12928	Mapping file id7702 NCBI file	EvidenceIEA, TAS

Evidence codes on this page: IEA, TAS, as the mapping file writes them; a row carrying two was written twice by the file, once under each. The mapping file id says which file placed the gene: an NCBI Gene id from NCBI2Reactome_All_Levels.txt, an Ensembl gene id from Ensembl2Reactome_All_Levels.txt. The two files can disagree about a code, so a row's codes are the one file's view. Measured by this site's build over this release on 2026-09-09: of the 138,908 human pathway-gene pairs both files place, 207 (0.149 per cent, over 47 genes) carry TAS in the Ensembl file where the NCBI rows carry IEA alone.

Reactome mapping files, the human rows for this pathway · Reactome release 97 · read 2026-09-09 · Reactome downloads"NCBI2Reactome_All_Levels.txt" and "Ensembl2Reactome_All_Levels.txt", Reactome, release 97, https://reactome.org/download-data/ (date of access 2026-09-09). Data CC0; attribution encouraged.

03The hierarchy

Parents and children in this release's hierarchy

Parents

Gene expression (Transcription)R-HSA-741601,545 genes

Children

Reactome's hierarchy is a graph rather than a tree: a pathway can sit under more than one parent, and the gene counts are each pathway's own placements at every level under it, so a parent's count is not the sum of its children's.

Reactome, the human pathway list and hierarchy relationship file · Reactome release 97 · read 2026-09-09 · Reactome downloads"ReactomePathways.txt" and "ReactomePathwaysRelation.txt", Reactome, release 97, https://reactome.org/download-data/ (date of access 2026-09-09). Data CC0; attribution encouraged.