Pathway Human Homo sapiens

Protein hydroxylation

R-HSA-9629569 in Reactome release 97: under Gamma carboxylation, hypusinylation, hydroxylation, and arylsulfatase activation, with 20 genes placed in it by the mapping files and 0 child pathways in the hierarchy.

The same number in the other species

Reactome writes the mouse and rat events it infers from a human pathway under the human number with the species token. A door opens only where that species' own list in this release holds the id: R-MMU-9629569 (mouse), R-RNO-9629569 (rat). Whether the event was inferred from this one is what the record says.

The record
The genes
Parents and children

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This page reads the pathway's record from its source while you read the rest; with scripts off the card stays at its loading line. Reload with scripts on to see it, or open the source's own page from the link in the card.

01The record

Reactome's own record of this pathway

What this tells you

The pathway list, the hierarchy and the genes on this page are read from the files Reactome publishes for Reactome release 97, built into this site on 2026-09-09: the pathway list, the hierarchy relationship file and the two gene mapping files. The record card is the one live read, Reactome's own record of this pathway.

Reactome's download page describes the mapping files: Mapping files link the source database identifier to the lowest level pathway diagram or subset of the pathway, all levels of the pathway hierarchy or database identifier to all reactions. [R13] The gene list here is the all-levels mapping of NCBI Gene ids, filled from the all-levels mapping of Ensembl ids where the NCBI file has no row for a gene; each row names which file it came from by the shape of its source id. Each row carries the evidence codes the file writes for it, as written and unranked, and both where the file writes both; no page of Reactome's documentation the survey read defines the codes, so this page does not expand them. A gene id the identity files do not name is kept as the source's own id with no door rather than turned into a symbol.

On citing what a search finds, Reactome says: It should be remembered that while we strive to contain the most current and accurate data, Reactome should not be used in citations where other primary sources of information are available. [R12] The record card prints the literature Reactome attaches to a curated pathway for that reason. The data are public domain: All data in the Reactome database and files derived from that data are licensed under the Creative Commons Public Domain Dedication (CC0). User may copy, modify, and distribute these data, even for commercial purposes, without asking for permission. Attribution is encouraged but not required. [R10] The pathway illustrations are licensed apart, under CC BY 4.0, and none is shown here.

A gene on this list is one Reactome places in this pathway in this release, and that is all the row says: it does not say the gene is expressed in a tissue or associated with a disease, which are the other two explorers' questions, read from other sources. Reactome's papers of record are [R01] [R02].

[R01] Ragueneau E, Gong C, Sinquin P, Sevilla C, Beavers D, Grentner A, et al. (2026). The Reactome Knowledgebase 2026. Nucleic Acids Research 54:D673-D681. PMID 41251150, doi 10.1093/nar/gkaf1223.
[R02] Milacic M, Beavers D, Conley P, Gong C, Gillespie M, Griss J, et al. (2024). The Reactome Pathway Knowledgebase 2024. Nucleic Acids Research 52:D672-D678. PMID 37941124, doi 10.1093/nar/gkad1025.
[R10] Reactome, reactome.org. License Agreement. https://reactome.org/license, read 2026-09-09.
[R12] Reactome, reactome.org. Citing us. https://reactome.org/cite, read 2026-09-09.
[R13] Reactome, reactome.org. Download. https://reactome.org/download-data, read 2026-09-09.

Stable id: R-HSA-9629569.1
Class: Pathway
Species: Homo sapiens (human)
Inferred: no: a curated human pathway
Disease pathway: no
Released: 2023-09-13
Events inside: 10, counted from the record

This pathway groups reactions mediated by 2-OG (2-oxoglutarate)-dependent oxygenases that target proline, lysine, asparagine, arginine, aspartate, and histidine residues of diverse proteins, with effects that potentially modulate transcription and translation (Herr & Hausinger 2018; Markolovic et al. 2015; Stoehr et al. 2016; Zurlo et al. 2016).

The roles of members of this enzyme family in collagen assembly are annotated separately in pathway R-HSA-1650814, "Collagen biosynthesis and modifying enzymes".

Literature Reactome attaches

Amazing Diversity in Biochemical Roles of Fe(II)/2-Oxoglutarate OxygenasesTrends Biochem. Sci., 2018, vol. 43, p. 517-532PubMed 29709390
Protein Hydroxylation Catalyzed by 2-Oxoglutarate-dependent OxygenasesJ. Biol. Chem., 2015, vol. 290, p. 20712-22PubMed 26152730
Prolyl hydroxylation regulates protein degradation, synthesis, and splicing in human induced pluripotent stem cell-derived cardiomyocytesCardiovasc. Res., 2016, vol. 110, p. 346-58PubMed 27095734
New Insights into Protein Hydroxylation and Its Important Role in Human DiseasesBiochim. Biophys. Acta, 2016, vol. 1866, p. 208-220PubMed 27663420

Reactome's record lists 14 orthologous events, R-RNO-9629569 (Rattus norvegicus), R-MMU-9629569 (Mus musculus) among the species this site serves, and 12 in species this site opens no page for.

Reactome ContentService, this pathway's record · Reactome v97 · read 2026-09-30 ET · Reactome pathway browser"Protein hydroxylation". Reactome, release 97, https://reactome.org/PathwayBrowser/#/R-HSA-9629569 with StableID: R-HSA-9629569.1 (accessed 2026-09-30 ET). Reactome version 97 (CC0).

Reading this pathway's record from Reactome (the pathway record).Still reading. A first read of a pathway can take a while; this page waits up to 35 seconds for it, and its scripts then bring in the panel, or a line saying what did not arrive.

02The genes

Genes Reactome places in this human pathway

The mapping files place 20 genes in this human pathway; showing 1 to 20, in pages of 100, sorted by symbol for reading. The order carries no ranking.

Genes Reactome places in this human pathway, page 1 of 1
Gene	Authority id	Mapping file id	Evidence codes
GeneASPH	AuthorityHGNC:757	Mapping file id444 NCBI file	EvidenceIEA
GeneDRG1	AuthorityHGNC:3029	Mapping file id4733 NCBI file	EvidenceTAS
GeneDRG2	AuthorityHGNC:3030	Mapping file id1819 NCBI file	EvidenceTAS
GeneETF1	AuthorityHGNC:3477	Mapping file id2107 NCBI file	EvidenceTAS
GeneF9	AuthorityHGNC:3551	Mapping file id2158 NCBI file	EvidenceIEA
GeneJMJD4	AuthorityHGNC:25724	Mapping file id65094 NCBI file	EvidenceTAS
GeneJMJD6	AuthorityHGNC:19355	Mapping file id23210 NCBI file	EvidenceTAS
GeneJMJD7	AuthorityHGNC:34397	Mapping file id100137047 NCBI file	EvidenceTAS
GeneKDM8	AuthorityHGNC:25840	Mapping file id79831 NCBI file	EvidenceTAS
GeneOGFOD1	AuthorityHGNC:25585	Mapping file id55239 NCBI file	EvidenceTAS
GeneRCCD1	AuthorityHGNC:30457	Mapping file id91433 NCBI file	EvidenceTAS
GeneRIOX1	AuthorityHGNC:20968	Mapping file id79697 NCBI file	EvidenceTAS
GeneRIOX2	AuthorityHGNC:19441	Mapping file id84864 NCBI file	EvidenceTAS
GeneRPL27A	AuthorityHGNC:10329	Mapping file id6157 NCBI file	EvidenceTAS
GeneRPL8	AuthorityHGNC:10368	Mapping file id6132 NCBI file	EvidenceTAS
GeneRPS23	AuthorityHGNC:10410	Mapping file id6228 NCBI file	EvidenceTAS
GeneRPS6	AuthorityHGNC:10429	Mapping file id6194 NCBI file	EvidenceTAS
GeneRWDD1	AuthorityHGNC:20993	Mapping file id51389 NCBI file	EvidenceTAS
GeneU2AF2	AuthorityHGNC:23156	Mapping file id11338 NCBI file	EvidenceTAS
GeneZC3H15	AuthorityHGNC:29528	Mapping file id55854 NCBI file	EvidenceTAS

Evidence codes on this page: IEA, TAS, as the mapping file writes them; a row carrying two was written twice by the file, once under each. The mapping file id says which file placed the gene: an NCBI Gene id from NCBI2Reactome_All_Levels.txt, an Ensembl gene id from Ensembl2Reactome_All_Levels.txt. The two files can disagree about a code, so a row's codes are the one file's view. Measured by this site's build over this release on 2026-09-09: of the 138,908 human pathway-gene pairs both files place, 207 (0.149 per cent, over 47 genes) carry TAS in the Ensembl file where the NCBI rows carry IEA alone.

Reactome mapping files, the human rows for this pathway · Reactome release 97 · read 2026-09-09 · Reactome downloads"NCBI2Reactome_All_Levels.txt" and "Ensembl2Reactome_All_Levels.txt", Reactome, release 97, https://reactome.org/download-data/ (date of access 2026-09-09). Data CC0; attribution encouraged.

03The hierarchy

Parents and children in this release's hierarchy

Parents

Gamma carboxylation, hypusinylation, hydroxylation, and arylsulfatase activationR-HSA-16384161 genes

Children

None: no pathway of this release's list names this one as a parent.

Reactome's hierarchy is a graph rather than a tree: a pathway can sit under more than one parent, and the gene counts are each pathway's own placements at every level under it, so a parent's count is not the sum of its children's.

Reactome, the human pathway list and hierarchy relationship file · Reactome release 97 · read 2026-09-09 · Reactome downloads"ReactomePathways.txt" and "ReactomePathwaysRelation.txt", Reactome, release 97, https://reactome.org/download-data/ (date of access 2026-09-09). Data CC0; attribution encouraged.